Cloning and characterization of a novel rice gene family encoding putative dual-specificity protein kinases, involved in plant responses to abiotic and biotic stresses

Plant Science ◽  
2005 ◽  
Vol 169 (3) ◽  
pp. 470-477 ◽  
Author(s):  
Zhimin Gu ◽  
Jianfei Wang ◽  
Ji Huang ◽  
Hongsheng Zhang
Keyword(s):  
Gene Family ◽  
Protein Kinases ◽  
Plant Responses ◽  
Rice Gene ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Dual Specificity ◽  
Specificity Protein

Identification of Plant Protein Kinases in Response to Abiotic and Biotic Stresses Using SuperSAGE

2011 ◽  
Vol 12 (7) ◽  
pp. 643-656 ◽  
Author(s):  
Ederson Akio Kido ◽  
Pedranne Kelle de Araujo Barbosa ◽  
Jose Ribamar Costa Ferreira Neto ◽  
Valesca Pandolfi ◽  
Laureen Michelle Houllou-Kido ◽  
...  
Keyword(s):  
Protein Kinases ◽  
Plant Protein ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses

scholarly journals Characterization of Caenorhabditis elegans Homologs of the Down Syndrome Candidate Gene DYRK1A

Genetics ◽  
2003 ◽  
Vol 163 (2) ◽  
pp. 571-580 ◽  
Author(s):  
William B Raich ◽  
Celine Moorman ◽  
Clay O Lacefield ◽  
Jonah Lehrer ◽  
Dusan Bartsch ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Caenorhabditis Elegans ◽  
Trisomy 21 ◽  
Gene Dosage ◽  
Inducible Promoters ◽  
C Elegans ◽  
Dual Specificity ◽  
Specificity Protein

Abstract The pathology of trisomy 21/Down syndrome includes cognitive and memory deficits. Increased expression of the dual-specificity protein kinase DYRK1A kinase (DYRK1A) appears to play a significant role in the neuropathology of Down syndrome. To shed light on the cellular role of DYRK1A and related genes we identified three DYRK/minibrain-like genes in the genome sequence of Caenorhabditis elegans, termed mbk-1, mbk-2, and hpk-1. We found these genes to be widely expressed and to localize to distinct subcellular compartments. We isolated deletion alleles in all three genes and show that loss of mbk-1, the gene most closely related to DYRK1A, causes no obvious defects, while another gene, mbk-2, is essential for viability. The overexpression of DYRK1A in Down syndrome led us to examine the effects of overexpression of its C. elegans ortholog mbk-1. We found that animals containing additional copies of the mbk-1 gene display behavioral defects in chemotaxis toward volatile chemoattractants and that the extent of these defects correlates with mbk-1 gene dosage. Using tissue-specific and inducible promoters, we show that additional copies of mbk-1 can impair olfaction cell-autonomously in mature, fully differentiated neurons and that this impairment is reversible. Our results suggest that increased gene dosage of human DYRK1A in trisomy 21 may disrupt the function of fully differentiated neurons and that this disruption is reversible.

scholarly journals In Silico Prediction and Functional Characterization of Genes Related to Abiotic and Biotic Stresses in Chickpea (Cicer arietinum)

2018 ◽  
Vol 7 (1) ◽  
pp. 1-35
Author(s):  
Sukhdeep Kaur ◽  
Satendra Singh ◽  
Gitanjali Tandon ◽  
Sarika Jaiswal ◽  
Mir Asif Iquebal ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
In Silico ◽  
Functional Characterization ◽  
In Silico Prediction ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses

scholarly journals Unveiling the Novel Dual Specificity Protein Kinases inBacillus anthracis

2012 ◽  
Vol 287 (32) ◽  
pp. 26749-26763 ◽  
Author(s):  
Gunjan Arora ◽  
Andaleeb Sajid ◽  
Mary Diana Arulanandh ◽  
Anshika Singhal ◽  
Abid R. Mattoo ◽  
...  
Keyword(s):  
Protein Kinases ◽  
The Novel ◽  
Dual Specificity ◽  
Specificity Protein

scholarly journals A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Antoine Larrieu ◽  
Antony Champion ◽  
Jonathan Legrand ◽  
Julien Lavenus ◽  
David Mast ◽  
...  
Keyword(s):  
High Resolution ◽  
Temporal Distribution ◽  
Spatiotemporal Data ◽  
Plant Responses ◽  
Spatial And Temporal Distribution ◽  
In Planta ◽  
Dynamic Changes ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Response To Stress

Abstract Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

scholarly journals Molecular cloning and characterization of a novel dual-specificity protein phosphatase possibly involved in spermatogenesis

1999 ◽  
Vol 344 (3) ◽  
pp. 819 ◽  
Author(s):  
Koji NAKAMURA ◽  
Hiroshi SHIMA ◽  
Masahiko WATANABE ◽  
Tatsuji HANEJI ◽  
Kunimi KIKUCHI
Keyword(s):  
Molecular Cloning ◽  
Protein Phosphatase ◽  
Dual Specificity ◽  
Dual Specificity Protein Phosphatase ◽  
Specificity Protein

scholarly journals The dual-specificity CLK kinase induces neuronal differentiation of PC12 cells.

1994 ◽  
Vol 14 (10) ◽  
pp. 6954-6961 ◽  
Author(s):  
M P Myers ◽  
M B Murphy ◽  
G Landreth
Keyword(s):  
Signal Transduction ◽  
Growth Factor ◽  
Protein Kinases ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Morphological Differentiation ◽  
Signal Transduction Pathways ◽  
Dual Specificity ◽  
Free Media ◽  
Specificity Protein

CLK is a dual-specificity protein kinase capable of phosphorylating serine, threonine, and tyrosine residues. We have investigated the action of CLK by establishing stable PC12 cell lines capable of inducibly expressing CLK. Expression of CLK in stably transfected PC12 cells mimicked a number of nerve growth factor (NGF)-dependent events, including the morphological differentiation of these cells and the elaboration of neurites. Moreover, CLK expression enhanced the rate of NGF-mediated neurite outgrowth of these cells, indicating that CLK expression and NGF treatment activate similar signal transduction pathways. CLK expression, unlike NGF, was not able to promote PC12 cell survival in serum-free media, demonstrating that CLK only partially recapitulated the actions of NGF on these cells and that the biochemical pathways necessary for morphological differentiation can be stimulated without also stimulating those necessary for survival. Induction of CLK expression also resulted in the selective activation of protein kinases that are components of growth factor-stimulated signal transduction cascades, including ERK1, ERK2, pp90RSK, and S6PKII. Induction of CLK expression, however, did not stimulate pp70S6K or Fos kinase, two NGF-sensitive protein kinases. These data indicate that CLK action mediates the morphological differentiation of these cells through its capacity to independently stimulate signal transduction pathways normally employed by NGF.

scholarly journals Expression, purification and characterization of recombinant mitogen-activated protein kinase kinases

1994 ◽  
Vol 303 (1) ◽  
pp. 105-112 ◽  
Author(s):  
P Dent ◽  
Y H Chow ◽  
J Wu ◽  
D K Morrison ◽  
R Jove ◽  
...  
Keyword(s):  
Peptide Mapping ◽  
Mitogen Activated Protein Kinase ◽  
Recombinant Viruses ◽  
Dual Specificity ◽  
Protein Map ◽  
Mitogen Activated Protein ◽  
Michaelis Constants ◽  
High Level ◽  
Specificity Protein

Mitogen-activated protein (MAP) kinase kinases (MKKs) are dual-specificity protein kinases which activate p42mapk and p44mapk by phosphorylation of regulatory tyrosine and threonine residues. cDNAs for two isotypes of MKK, MKK1 and MKK2, have been isolated from several species. Here we describe construction of recombinant baculoviruses for high-level expression of histidine-tagged rat MKK1 and MKK2, and procedures for production of nearly homogeneous MKK1 and MKK2 fusion proteins, in both inactive and active forms. Co-infection of Sf9 cells with either MKK1 or MKK2 virus together with recombinant viruses for Raf-1, pp60src (Y527F) and c-Ha-Ras resulted in activations of 250-fold and 150-fold for MKK1 and MKK2 respectively. Specific activities towards kinase-defective p42mapk were of the order of several hundred nanomoles of phosphate transferred/min per mg of MKK protein. The Michaelis constants for both enzymes were approx. 1 microM. Preparations of activated MKK were apparently free of Raf-1 as assessed by Western blotting. Raf-1 phosphorylated MKK1 on one major tryptic phosphopeptide, the phosphorylation of which increased with time. This phosphopeptide contained only phosphoserine and possessed neutral overall charge at pH 1.9 on two-dimensional peptide mapping. Phosphorylation of MKK1 by Raf-1 correlated with activation and reached a plateau of approximately 2 mol/mol.

scholarly journals Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L.

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247170
Author(s):  
Md. Soyib Hasan ◽  
Vishal Singh ◽  
Shiful Islam ◽  
Md. Sifatul Islam ◽  
Raju Ahsan ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Expression Profiling ◽  
Developmental Stages ◽  
Functional Characterization ◽  
Drought Treatment ◽  
Specific Expression ◽  
Abiotic And Biotic Stresses ◽  
Biotic Stresses ◽  
Genome Wide

Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well as plant development and growth. The present study aimed to a comprehensive genome-wide identification and functional characterization of GST family in one of the economically important legume plants—Medicago truncatula. Here, we have identified a total of ninety-two putative MtGST genes that code for 120 proteins. All these members were classified into twelve classes based on their phylogenetic relationship and the presence of structural conserved domain/motif. Among them, 7 MtGST gene pairs were identified to have segmental duplication. Expression profiling of MtGST transcripts revealed their high level of organ/tissue-specific expression in most of the developmental stages and anatomical tissues. The transcripts of MtGSTU5, MtGSTU8, MtGSTU17, MtGSTU46, and MtGSTU47 showed significant up-regulation in response to various abiotic and biotic stresses. Moreover, transcripts of MtGSTU8, MtGSTU14, MtGSTU28, MtGSTU30, MtGSTU34, MtGSTU46 and MtGSTF8 were found to be highly upregulated in response to drought treatment for 24h and 48h. Among the highly stress-responsive MtGST members, MtGSTU17 showed strong affinity towards its conventional substrates reduced glutathione (GSH) and 1‐chloro‐2,4‐dinitrobenzene (CDNB) with the lowest binding energy of—5.7 kcal/mol and -6.5 kcal/mol, respectively. Furthermore, the substrate-binding site residues of MtGSTU17 were found to be highly conserved. These findings will facilitate the further functional and evolutionary characterization of GST genes in Medicago.

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