Evaluation of suitable reference genes for normalization of microRNA expression by real-time reverse transcription PCR analysis during longan somatic embryogenesis

2013 ◽  
Vol 66 ◽  
pp. 20-25 ◽  
Author(s):  
Yu Ling Lin ◽  
Zhong Xiong Lai
Keyword(s):  
Somatic Embryogenesis ◽  
Real Time ◽  
Reverse Transcription ◽  
Reference Genes ◽  
Microrna Expression ◽  
Pcr Analysis ◽  
Reverse Transcription Pcr ◽  
Suitable Reference

scholarly journals Screening Suitable Reference Genes for Normalization in Reverse Transcription Quantitative Real-Time PCR Analysis in Melon

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e87197 ◽  
Author(s):  
Qiusheng Kong ◽  
Jingxian Yuan ◽  
Penghui Niu ◽  
Junjun Xie ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Quantitative Real Time Pcr ◽  
Suitable Reference

scholarly journals Selection of reference genes for gene expression analysis in Liriodendron hybrids’ somatic embryogenesis and germinative tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tingting Li ◽  
Weigao Yuan ◽  
Shuai Qiu ◽  
Jisen Shi
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Reference Genes ◽  
Gene Selection ◽  
Elongation Factor ◽  
Reverse Transcription Pcr ◽  
Expression Of Genes ◽  
Suitable Reference ◽  
Functional Analyses ◽  
Elongation Factor 1

AbstractThe differential expression of genes is crucial for plant somatic embryogenesis (SE), and the accurate quantification of gene expression levels relies on choosing appropriate reference genes. To select the most suitable reference genes for SE studies, 10 commonly used reference genes were examined in synchronized somatic embryogenic and subsequent germinative cultures of Liriodendron hybrids by using quantitative real-time reverse transcription PCR. Four popular normalization algorithms: geNorm, NormFinder, Bestkeeper and Delta-Ct were used to select and validate the suitable reference genes. The results showed that elongation factor 1-gamma, histone H1 linker protein, glyceraldehyde-3-phosphate dehydrogenase and α-tubulin were suitable for SE tissues, while elongation factor 1-gamma and actin were best for the germinative organ tissues. Our work will benefit future studies of gene expression and functional analyses of SE in Liriodendron hybrids. It is also serves as a guide of reference gene selection in early embryonic gene expression analyses for other woody plant species.

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