Expression patterns of genes encoding plasma membrane aquaporins during fruit development in cucumber ( Cucumis sativus L.)

2015 ◽  
Vol 96 ◽  
pp. 329-336 ◽  
Author(s):  
Jin Shi ◽  
Jinfang Wang ◽  
Ren Li ◽  
Dianbo Li ◽  
Fengfeng Xu ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cucumis Sativus ◽  
Fruit Development ◽  
Expression Patterns ◽  
Cucumis Sativus L ◽  
Genes Encoding

scholarly journals The influence of different moisture contents and nitrogen levels on the formation of empty cavities in cucumbers (Cucumis sativus L.)

2013 ◽  
Vol 35 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Krystyna Elkner
Keyword(s):  
Soil Moisture ◽  
Cucumis Sativus ◽  
Nitrogen Fertilization ◽  
Fruit Development ◽  
High Nitrogen ◽  
Mechanical Tension ◽  
Cucumis Sativus L ◽  
Nitrogen Levels ◽  
Moisture Contents ◽  
Empty Cavity

Empty cavities were found already in very young cucumber fruits with diameter 1.5 cm. As the fruit develops the empty cavities augment and the number of fruits showing this defect increases. Low soil moisture and high nitrogen fertilization favour the formation of empty cavities. Their origination and changes were traced with anatomical methods in the course of fruit development. As a most plausible cause of their origination the author considers the enlargement of only part of the cells of the suture between the two (or three) carples. Due to this uneven enlargement of neighbouring cells strong mechanical tension probably arises amoung them, leading to the formation of ruptures separating these cells, consequently causing the separation of the carpel edges. Besides that, some of the cells of the suture which have markedly enlarged, often burst which also contributes to the formation of an empty cavity and enlarges its dimensions.

scholarly journals Genome-Wide Characterization of GRAS Family and Their Potential Roles in Cold Tolerance of Cucumber (Cucumis sativus L.)

2020 ◽  
Vol 21 (11) ◽  
pp. 3857 ◽  
Author(s):  
Xiaohong Lu ◽  
Wenqian Liu ◽  
Chenggang Xiang ◽  
Xiaojun Li ◽  
Qing Wang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Gene Family ◽  
Cold Tolerance ◽  
Cucumis Sativus ◽  
Expression Patterns ◽  
Cold Treatment ◽  
Evolutionary Tree ◽  
Cucumis Sativus L ◽  
Gras Family ◽  
Biological Stress

Cucumber (Cucumis sativus L.) is one of the most important cucurbit vegetables but is often subjected to stress during cultivation. GRAS (gibberellic acid insensitive, repressor of GAI, and scarecrow) genes encode a family of transcriptional factors that regulate plant growth and development. In the model plant Arabidopsis thaliana, GRAS family genes function in formation of axillary meristem and root radial structure, phytohormone (gibberellin) signal transduction, light signal transduction and abiotic/biological stress. In this study, a gene family was comprehensively analyzed from the aspects of evolutionary tree, gene structure, chromosome location, evolutionary and expression pattern by means of bioinformatics; 37 GRAS gene family members have been screened from cucumber. We reconstructed an evolutionary tree based on multiple sequence alignment of the typical GRAS domain and conserved motif sequences with those of other species (A. thaliana and Solanum lycopersicum). Cucumber GRAS family was divided into 10 groups according to the classification of Arabidopsis and tomato genes. We conclude that tandem and segmental duplication have played important roles in the expansion and evolution of the cucumber GRAS (CsaGRAS) family. Expression patterns of CsaGRAS genes in different tissues and under cold treatment, combined with gene ontology annotation and interaction network analysis, revealed potentially different functions for CsaGRAS genes in response to cold tolerance, with members of the SHR, SCR and DELLA subfamilies likely playing important roles. In conclusion, this study provides valuable information and candidate genes for improving cucumber tolerance to cold stress.

Characterization of plasma membrane bound cation-stimulated ATPase isolated from the roots of Cucumis sativus

1988 ◽  
Vol 66 (7) ◽  
pp. 1470-1473 ◽  
Author(s):  
Abdul Razaque Memon
Keyword(s):  
Plasma Membrane ◽  
Cucumis Sativus ◽  
Direct Interaction ◽  
Monovalent Cation ◽  
Phase System ◽  
Plasma Membrane Atpase ◽  
Cucumis Sativus L ◽  
Membrane Bound ◽  
Cucumber Roots

A membrane fraction enriched with Mg2+- or Mn2+-dependent, monovalent cation stimulated ATPase was isolated from cucumber roots (Cucumis sativus L.) by an aqueous two-polymer phase system of Dextran T500 (6.5%, w/w) and polyethylene glycol (PEG) 3350 (6.5%, w/w) at pH 7.8. The ATPase activity associated with the upper PEG-rich fraction (plasma membrane) was characterized. The optimum pH for the activation by Mg2+ and Mn2+ was in the range 5.8–6.0. The activity was substrate specific for ATP. Kinetics with Mg2+ or Mn2+ followed a simple Michaelis–Menten relationship. The apparent Km for Mg2+ activation (0.60 mM) of the ATPase was about twice that of the apparent Km for Mn2+ (0.38 mM). ATPase was stimulated by monovalent cations and showed an order of cation preference of [Formula: see text]. Calcium inhibited the plasma membrane ATPase, apparently by a direct interaction with ATPase rather than by disrupting the MgATP2− complex.

scholarly journals Benzaldehyde Synthases Are Encoded by Cinnamoyl-CoA Reductase Genes in Cucumber (Cucumis sativus L.)

2019 ◽  
Author(s):  
Baoxiu Liu ◽  
Guo Wei ◽  
Zhongyi Hu ◽  
Guodong Wang
Keyword(s):  
Cucumis Sativus ◽  
Expression Pattern ◽  
Lignin Biosynthesis ◽  
Specific Expression ◽  
Cucumis Sativus L ◽  
Genes Encoding ◽  
Long Time ◽  
Specific Expression Pattern ◽  
Coa Reductase

AbstractBenzaldedyde, commonly detected in plant VOC (volatile organic compounds) profiling, is derived from phenylalanine. However, the last enzymatic step for benzaldedyde formation, designated as benzaldehyde synthase, remains elusive for long time. Here, we demonstrated that cinnamoyl-CoA reductases are responsible for benzaldedyde production in cucumber (Cucumis sativus L.). Comprehensive tissue specificity of VOC profiling revealed that benzaldehyde was specifically accumulated in root and flower of cucumber plants. VOC-gene correlation analysis suggested that several CCRs are candidate genes for benzaldehyde production: CsaCCR7 had a root-specific expression pattern while CsaCCR9 and CsaCCR18 showed a flower-specific expression pattern. Enzymatic assay demonstrated that CsaCCR7, CsaCCR9 and CsaCCR18 convert benzoyl-CoA to benzaldehyde. Subcellular localization experiments revealed that CsaCCR7 and CsaCCR18 are localized in cytosol, while CsaCCR9 was localized in peroxisome. In contrast to the long-standing view that CCR enzymes are involved in lignin biosynthesis in plants, it is the first time here to add a new biochemical role of CCR as benzaldehyde synthase in plants.HighlightsBenzaldehyde is mainly produced in flower and root of cucumber plants.14 genes encoding CCR enzyme from cucumber are comprehensively analyzed.Three CsaCCRs, function as benzaldehyde synthases, utilize benzoyl-CoA as substrate to produce benzaldehyde in vitro.

Effect of Cephalexin on Sex Expression, Fruit Development and Yield of Cucumber (Cucumis sativus L.)

2002 ◽  
Vol 2 (10) ◽  
pp. 656-658
Author(s):  
M.A. Hossain . ◽  
M.R. Karim . ◽  
S. Begum . ◽  
M.A. Hossain . ◽  
M.A. Haque .
Keyword(s):  
Cucumis Sativus ◽  
Fruit Development ◽  
Sex Expression ◽  
Cucumis Sativus L

scholarly journals Comprehensive Characterization of Fruit Volatiles and Nutritional Quality of Three Cucumber (Cucumis sativus L.) Genotypes from Different Geographic Groups after Bagging Treatment

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 294 ◽  
Author(s):  
Nan Shan ◽  
Zengyu Gan ◽  
Jing Nie ◽  
Huan Liu ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Nutritional Quality ◽  
Expression Patterns ◽  
Relative Proportion ◽  
Soluble Sugars ◽  
Hydroperoxide Lyase ◽  
Cucumis Sativus L ◽  
Cucumber Fruit ◽  
Fruit Flavor

Bagging is widely practiced to produce high quality and unblemished fruit; however, little is currently known about the effect of bagging on flavor and nutritional quality of cucumber fruits. Here we determined the influence of bagging on fruit quality of cucumber (Cucumis sativus L.) using three genotypes from different geographic groups. Exocarp chlorophyll and carotenoid levels were significantly decreased by bagging, accompanied by color change. Ascorbate content in bagged fruits decreased to some extent, while contents of soluble sugars, starch, and cellulose were comparable with those of control fruits. Compositions related to fruit flavor quality could be enhanced largely through bagging treatment, with elevation of the relative proportion of C6 aldehyde, as well as (E,Z)-2,6-nonadienal/(E)-2-nonenal ratio, and linoleic/α-linolenic acid ratio. Lipoxygenase and hydroperoxide lyase, two key enzymes in the production of volatiles, displayed distinctive transcript expression patterns and trends in changes of enzymatic activity in the bagged fruits of different genotypes. Overall, this study assesses the information on changing characteristics of fruit volatile composition and nutritional quality among different cucumber genotypes after bagging treatment. Results of this study would contribute to providing reference for mechanism study and cultivation conditions to improve cucumber fruit flavor to a considerable degree.

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