Expression of the pyrroline-5-carboxylate reductase (P5CR) gene from the wild grapevine Vitis yeshanensis promotes drought resistance in transgenic Arabidopsis

Author(s):  
Chengcheng Chen ◽  
Xiaoyue Cui ◽  
Pingying Zhang ◽  
Zheng Wang ◽  
Jianxia Zhang
PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168040 ◽  
Author(s):  
Jingling Yu ◽  
Lei Yang ◽  
Xiaobing Liu ◽  
Renjie Tang ◽  
Yuan Wang ◽  
...  

2020 ◽  
Vol 21 (7) ◽  
pp. 2624
Author(s):  
Yumeng Cai ◽  
Jing Yan ◽  
Wenrui Tu ◽  
Zhefang Deng ◽  
Wenjie Dong ◽  
...  

Sucrose is the predominant form of sugar transported from photosynthetic (source) to non-photosynthetic (sink) organs in higher plants relying on the transporting function of sucrose transporters (SUTs or SUCs). Many SUTs have been identified and characterized in both monocots and dicots. However, the function of sucrose transporters (SUTs or SUCs) from Vitis is not clear. As the world’s most planted grape species, Vitis vinifera owns three sucrose transport activity verified SUTs. In this study, we constructed three kinds of VvSUC (Vitis vinifera SUC)-overexpressing transgenic Arabidopsis. VvSUC-overexpressing transgenic Arabidopsis was cultured on sucrose-supplemented medium. VvSUC11- and VvSUC12-overexpressing lines had similar thrived growth phenotypes, whereas the size and number of leaves and roots from VvSUC27-overexpressing lines were reduced compared with that of WT. When plants were cultured in soil, all SUT transgenic seedlings produced more number of leaves and siliques, resulting in higher yield (38.6% for VvSUC12-transformants) than that of WT. Besides, VvSUC27-transformants and VvSUC11-transformants enhanced drought resistance in Arabidopsis, providing a promising target for crop improvement


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