Comparative proteome profiling of susceptible and resistant rice cultivars identified an arginase involved in rice defense against Xanthomonas oryzae pv. oryzae

The hrp gene cluster of Xanthomonas spp. contains genes for the assembly and function of a type III secretion system (TTSS). The hrpF genes reside in a region between hpaB and the right end of the hrp cluster. The region of the hrpF gene of Xanthomonas oryzae pv. oryzae is bounded by two IS elements and also contains a homolog of hpaF of X. campestris pv. vesicatoria and two newly identified genes, hpa3 and hpa4. A comparison of the hrp gene clusters of different species of Xanthomonas revealed that the hrpF region is a constant yet more variable peninsula of the hrp pathogenicity island. Mutations in hpaF, hpa3, and hpa4 had no effect on virulence, whereas hrpF mutants were severely reduced in virulence on susceptible rice cultivars. The hrpF genes from X. campestris pv. vesicatoria, X. campestris pv. campestris, and X. axonopodis pv. citri each were capable of restoring virulence to the hrpF mutant of X. oryzae pv. oryzae. Correspondingly, none of the Xanthomonas pathovars with hrpF from X. oryzae pv. oryzae elicited a hypersensitive reaction in their respective hosts. Therefore, no evidence was found for hrpF as a host-specialization factor. In contrast to the loss of Bs3-dependent reactions by hrpF mutants of X. campestris pv. vesicatoria, hrpF mutants of X. oryzae pv. oryzae with either avrXa10 or avrXa7 elicited hypersensitive reactions in rice cultivars with the corresponding R genes. A double hrpFxoo-hpa1 mutant also elicited an Xa10-dependent resistance reaction. Thus, loss of hrpF, hpa1, or both may reduce delivery or effectiveness of type III effectors. However, the mutations did not completely prevent the delivery of effectors from X. oryzae pv. oryzae into the host cells.

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A Widely Distributed Lineage of Xanthomonas oryzae pv. oryzae in India May Have Come from Native Wild Rice

Plant Disease ◽

10.1094/pdis.2000.84.4.465 ◽

2000 ◽

Vol 84 (4) ◽

pp. 465-469 ◽

Cited By ~ 6

Author(s):

J. Yashitola ◽

A. P. K. Reddy ◽

Ramesh V. Sonti

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Wild Rice ◽

Andhra Pradesh ◽

Fragment Length Polymorphism ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Rice Cultivars ◽

Cultivated Rice ◽

Serious Disease ◽

Restriction Fragment Length

Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. We have collected leaf blight-affected samples from wild rice (Oryza nivara) plants growing naturally at 22 locations in five revenue districts (Nalgonda, Ranga Reddy, Medak, Nizamabad, and Adilabad) in the Telangana Region of Andhra Pradesh, India. Pathotype analysis on a set of differential rice cultivars and DNA fingerprinting with two multilocus restriction fragment length polymorphism probes indicated that the X. oryzae pv. oryzae strains from the O. nivara plants belonged to a pathotype and lineage previously widely distributed among cultivated rice in India. This suggests that the lineage may be native to wild rice and may have been transferred subsequently to cultivated rice plants.

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Differential expression of Xoo-induced kinase 1 (XIK1), a Xanthomonas oryzae pv. oryzae responsive gene, in bacterial blight-susceptible and Xa21-mediated resistant indica rice cultivars

Agriculture and Natural Resources ◽

10.34044/j.anres.2019.53.4.02 ◽

2019 ◽

Keyword(s):

Differential Expression ◽

Bacterial Blight ◽

Indica Rice ◽

Xanthomonas Oryzae ◽

Rice Cultivars ◽

Responsive Gene

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Growth Complementation of hrpXo Mutants of Xanthomonas oryzae pv. oryzae by Virulent Strains in Rice Cultivars Resistant and Susceptible to the Parental Strain

Journal of General Plant Pathology ◽

10.1007/pl00012987 ◽

2001 ◽

Vol 67 (1) ◽

pp. 51-57 ◽

Cited By ~ 17

Author(s):

Seiji TSUGE ◽

Ayako FURUTANI ◽

Rie FUKUNAKA ◽

Yasuyuki KUBO ◽

Osamu HORINO

Keyword(s):

Parental Strain ◽

Xanthomonas Oryzae ◽

Rice Cultivars ◽

Virulent Strains

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Ketahanan Berbagai Kultivar Padi Lokal terhadap Penyakit Hawar Daun Bakteri

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.12.3.89 ◽

2016 ◽

Vol 12 (3) ◽

pp. 89

Author(s):

Andi Khaeruni ◽

Erwin Najamuddin ◽

Teguh Wijayanto ◽

Syair Syair

Keyword(s):

Control Strategies ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Rice Cultivars ◽

Rice Varieties ◽

Resistant Varieties ◽

Rice Disease ◽

Generative Phase ◽

Blight Disease ◽

Screen House

Bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae is an important rice disease in Indonesia, including in South East Sulawesi. The use of resistant varieties is one of the effective and environmentally friendly control strategies to suppress the disease. This study aimed to determine the level of resistance of some local rice varieties of South and Southeast Sulawesi against Xanthomonas oryzae pv. oryzae pathotypes IV, VIII and X. The study was conducted in a screen house involving 3 bacterial pathotypes and 11 local rice cultivars. Shearing method was used for inoculation of bacteria to leaf on vegetative and generative phases. Disease incidences were measured 3 weeks after inoculation, and the data was further used to determine the level of resistance of the tested rice cultivars. The results showed that incubation period of the disease was longer on Kelaca cultivar compared to other cultivars. On vegetative phase, this cultivar showed moderate resistant to pathotypes IV and X, and highly resistant to patotype VIII, whereas on the generative phase it showed moderate resistant to pathotypes IV and VII, and highly resistant to pathotype X. Therefore, Kelaca cultivar can be recommended for endemic areas of leaf blight in South and Southeast Sulawesi

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Gene Analysis of Resistance to Bacterial Blight, Xanthomonas oryzae pv, oryzae in Korean Six Rice Cultivars

Research in Plant Disease ◽

10.5423/rpd.2004.10.1.073 ◽

2004 ◽

Vol 10 (1) ◽

pp. 73-77 ◽

Cited By ~ 1

Author(s):

Jin-Ah Ryuk ◽

Chun-Hwan Choi ◽

Hee-Kyoung Kang ◽

Jae-Eul Choi

Keyword(s):

Bacterial Blight ◽

Xanthomonas Oryzae ◽

Gene Analysis ◽

Rice Cultivars

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Genetic Analysis and Molecular Identification of Virulence in Xanthomonas oryzae pv. oryzae Isolates

ISRN Molecular Biology ◽

10.1155/2013/160157 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Amos Onasanya ◽

R. O. Onasanya ◽

Abiodun A. Ojo ◽

B. O. Adewale

Keyword(s):

Genetic Analysis ◽

Molecular Typing ◽

Genetic Information ◽

Genomic Dna ◽

Durable Resistance ◽

West African ◽

Xanthomonas Oryzae ◽

Rice Cultivars ◽

African Countries ◽

Rice Ecologies

Bacterial leaf blight (BLB) of rice is a very destructive disease worldwide and is caused by Xanthomonas oryzae pv. oryzae (Xoo). The aim of the present study was to examine if the Xoo virulence pathotypes obtained using phenotypic pathotyping could be confirmed using molecular approach. After screening of 60 Operon primers with genomic DNA of two Xoo isolates (virulent pathotype, Vr, and mildly virulent pathotype, MVr), 12 Operon primers that gave reproducible and useful genetic information were selected and used to analyze 50 Xoo isolates from 7 West African countries. Genetic analysis revealed two major Xoo virulence genotypes (Mta and Mtb) with Mta having two subgroups (Mta1 and Mta2). Mta1 (Vr1) subgroup genotype has occurrence in six countries and Mta2 (Vr2) in three countries while Mtb genotype characterized mildly virulence (MVr) Xoo isolates present in five countries. The study revealed possible linkage and correlation between phenotypic pathotyping and molecular typing of Xoo virulence. Xoo virulence genotypes were known to exist within country and there was evidence of Xoo pathogen migration between countries. Durable resistance rice cultivars would need to overcome both Mta and Mtb Xoo virulence genotypes in order to survive after their deployment into different rice ecologies in West Africa.

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