Smoking and red blood cell phospholipid membrane fatty acids

Abstract Objectives The purpose of this study was to use a newly validated method for measuring 21 cis-fatty acids in red blood cell (RBC) membranes to investigate race-ethnic and gender differences in saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA). In addition, two long-chain PUFA, namely, eicosapentaenoic (EPA) and docosahexaenoic acid (DHA), were summed to provide an Omega-3 Index for each participant. This index is considered a cardiovascular risk factor. Methods Units (n = 120) of whole blood in EDTA were purchased from BioIVT (Westbury, NY). The demographic make-up of the set was 60:60 men: women. Of the 120 blood donors, 37% were black, 29% were white, and 34% were Hispanic. Average ages were 44 y (black), 48 y (white), and 43 y (Hispanic). Upon arrival, units were washed, treated with 1% BHT, and packed RBC were frozen until time of analysis. Hydrolysis of esters, derivitization with pentafluorobenzyl bromide, gas chromatography, and mass spectrometric detection were carried out to measure the 21 most abundant cis-fatty acid concentrations in RBC, which were converted to weight percentages of total. For the Omega-3 Index, the percentage of totals calculated for EPA and DHA were summed. Results There were few small (≤1%) race-ethnic differences and no gender differences in the proportions of fatty acids as SFA, MUFA, or PUFA; overall these averaged (SD) 44% (1%), 16% (1%), and 40% (1%), respectively. The Omega-3 Index averaged (SD) 3.5% (1%). One blood donor reached the suggested goal of ≥ 8%, which is associated with low risk for cardiac events. Conclusions This small study was undertaken in advance of the U.S. nationally representative survey, NHANES 2019–2020, in which RBC membrane fatty acids will be measured in the same laboratory. It will be interesting to learn whether NHANES will confirm the mostly null demographic findings in RBC fatty acid percentages and the low average Omega-3 Index. Funding Sources None.

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A comparative validation of a child food frequency questionnaire using red blood cell membrane fatty acids

European Journal of Clinical Nutrition ◽

10.1038/ejcn.2012.26 ◽

2012 ◽

Vol 66 (7) ◽

pp. 825-829 ◽

Cited By ~ 38

Author(s):

T Burrows ◽

B Berthon ◽

M L Garg ◽

C E Collins

Keyword(s):

Fatty Acids ◽

Cell Membrane ◽

Blood Cell ◽

Red Blood Cell ◽

Food Frequency Questionnaire ◽

Food Frequency ◽

Red Blood Cell Membrane ◽

Membrane Fatty Acids ◽

Comparative Validation

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The association between red blood cell membrane fatty acids, oxidative stress and inflammatory response after coronary angioplasty

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2013.08.056 ◽

2013 ◽

Vol 65 ◽

pp. S40

Author(s):

V. Muzakova ◽

T. Cermak ◽

J. Matejka ◽

J. Skalicky ◽

X. Stramova ◽

...

Keyword(s):

Oxidative Stress ◽

Fatty Acids ◽

Cell Membrane ◽

Inflammatory Response ◽

Blood Cell ◽

Red Blood Cell ◽

Coronary Angioplasty ◽

Red Blood Cell Membrane ◽

Membrane Fatty Acids

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PSXI-32 Effects of algae DHA on fatty acid profile of plasma red blood cell membrane and fecal microbiota of adult cats

Journal of Animal Science ◽

10.1093/jas/skaa278.569 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 319-319

Author(s):

Carrie James ◽

Sandra L Rodriguez-Zas ◽

Maria R C de Godoy

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Blood Cell ◽

Fish Oil ◽

Red Blood Cell ◽

Fecal Microbiota ◽

Fatty Acid Profiles ◽

Male Adult ◽

Poultry Fat ◽

Adult Cats

Abstract There is evidence that algae can be a sustainable alternative of omega-3 polyunsaturated fatty acids (w-3 PUFA; DHA and EPA) in the diets of felines, but more information is needed to determine bioavailability of algal w-3 PUFAs in felines. Therefore, the objective of this study was to determine the effects of dietary supplementation of algae DHA on plasma and red blood cell (RBC) membrane fatty acid profiles and fecal microbiota of adult cats. A complete randomized design was utilized with thirty female and male adult cats (mean age: 1.8 ± 0.03 yr, mean BW: 4.5 ± 0.8 kg) which were fed an assigned diet for 90 d. Three diets were formulated with poultry fat alone or inclusion of 2% fish oil or 2% algae DHA meal. Blood samples were collected after fasting on 0, 30, 60 and 90 d to be analyzed for plasma and red blood cell fatty acid profiles. A fresh fecal sample was collected within 15 min of defecation from each cat to be analyzed for fecal microbiota. Illumina 16S rRNA sequencing from V4 region was completed using MiSeq and analyzed using QIIME 2. Plasma and RBC fatty acid concentrations at baseline were similar among all cats and treatment groups. However, dietary treatment had a significant effect on the concentrations of several fatty acids in plasma and RBC over time. Plasma and RBC concentrations of DHA were greater (P < 0.05) for cats fed the algal DHA diet compared to the control and fish oil diets. Conversely, plasma and RBC concentrations of EPA did not differ among treatments when analyzed as a change from baseline. Beta- and alpha-diversity did not differ among treatments, indicating that 2% fish oil or algal-DHA meal does alter fecal microbiota of cats in contrast with cats fed a poultry fat-based diet.

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