232 Effects of Yarrowia Lipolytica Supplementation on Growth Performance and Apparent Ileal Digestibility of Diets Fed to Nursery Pigs

The objective was to evaluate Yarrowia lipolytica (YL) as a lipid supplement fed to nursery pigs for the growth performance and apparent ileal digestibility (AID). Twenty-four pigs weaned at 21 d of age (12 barrows and 12 gilts with initial BW at 7.2 ± 0.6 kg) were allotted to 3 dietary treatments (n = 8) based on the randomized complete block design with sex and BW as blocks. The main effect was the dietary supplementation of YL (0, 1.5, and 3.0%, replacing poultry fat) with nutrients meeting or exceeding the requirements by NRC (2012) and fed to pigs for 21 d based on 2 phases. Feed intake and BW were recorded at d 0, 10, and 21 to calculate ADG, ADFI, and G:F. Fecal scores were recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect ileal digesta for AID of nutrients in diets. Data were analyzed using Proc Mixed of SAS. There were no differences in growth performance among treatments. Whereas, pigs fed 0 and 1.5% YL had higher (P < 0.05) AID of DM (55.0 and 52.2% to 40.2%, respectively) and GE (62.1 and 60.6% to 49.8%, respectively) than pigs fed 3% YL. There were no differences in AID of DM and GE between the pigs fed 0% and 1.5% YL. In conclusion, 1.5% Yarrowia lipolytica could be supplemented in nursery pig diets reducing 0.8% of poultry fat without affecting growth performance and AID of DM and GE.

Use of agro-industrial residues for lipase production by Candida viswanathii in a solid-state cultivation system

Lipases are an important group of enzymes that catalyze the hydrolysis of triacylglycerol and there are many industrial applications. The aim of this work was to produce the lipase by the yeast Candida viswanathii using solid state culture with agro-industrial wastes (barley bagasse, corn husk, corncob, soybean seed coat and soybean husk). The biomass pretreatment methods were evaluated, as well as the media supplementation with nitrogen and mixing substrates. Also, the efficiency of olive oil and poultry fat was evaluated on the induction of lipase production, followed by the scale-up from 20 g to 100 g. The enzyme activities in the cultures without pretreatement were higher when soybean seed coat supplemented with both olive oil (7.06 U/gss) and poultry fat (8.40 U/gss) were used. However, the pretreated substrates did not demonstrate a satisfying induction of lipolytic activity. From the nitrogen sources, yeast extract showed an increase of approximately twice the original production with both olive oil (18.12 U/gss) and poultry fat (15.98 U/gss) supplementation. On the scale-up step, the results demonstrated that, for the 20 g culture, the best lipase production was observed on the 7th day (33.52 U/gss), while for the 100 g culture the highest lipase activity was after 5 days (17.88 U/gss). The cultivation of ground soybean skin without pretreatment supplemented with yeast extract as a source of nitrogen, with fresh barley bagasse and poultry fat was the best combination.

Soybean Oil Replacement by Poultry Fat in Broiler Diets: Performance, Nutrient Digestibility, Plasma Lipid Profile and Muscle Fatty Acids Content

Continuous genetic improvements of commercial broiler strains has led to the necessity of using fats in their rations to fulfill a large portion of the energetic requirements. Several fat sources have been introduced in poultry nutrition, such as rendering poultry fat (PF) an available and cheap lipid source compared to conventional sources such as soybean oil (SO). The present study investigated the effect of partial or full replacement of SO by PF on performance, nutrient digestibility, blood lipids, and fatty acids (FAs) content of pectoral muscle. Four hundred and eighty one-day-old male Ross-308 chicks were distributed into four experimental groups (12 replicates each): the first group (control) was fed a diet formulated with soybean oil as a fat source while the second to fourth groups (PF25, PF50, and PF100) were fed diets formulated with 25, 50 and 100% of PF as a fat source instead of SO. Results revealed no synergistic effect between SO and PF in any of the studied parameters. Replacing SO by PF did not alter birds’ growth, carcass characteristics, and plasma indices of birds. Abdominal fat% was increased (p < 0.01) in PF50 and PF100. Dry matter digestibility was improved (p < 0.05) in PF50 and PF100, while crude fat and protein digestibility was not affected. Contents of palmitic and docosahexaenoic acids in the pectoral muscle of PF50 and PF100 were reduced (p < 0.01) while concentrations of oleic and linolenic acids, total unsaturated FAs, and polyunsaturated FAs/Saturated FAs ratio were elevated (p < 0.05) in the same groups. Liver thiobarbituric acid reactive substances (TBARS) and muscle vitamin E contents were not altered. The dietary addition of PF greatly improved economic parameters. In conclusion, PF can be used as a lipid source in broiler diets to produce inexpensive meat while maintaining its growth performance.

Effects of moisture and temperature on Salmonella survivability in beef tallow, white grease and chicken rendered fat

High moisture levels introduced to fats after the rendering process can lead to Salmonella presence and growth. Limited research on strategies to eliminate pathogens in these environments are available. Rendered fat characteristics, such as water activity and fatty acids composition, may contribute to Salmonella survivability. The purpose of this research was to evaluate the effects of moisture levels (0%, 0.5%, 1%, and 3%), storage temperatures (48˚C and 76˚C), and fat characteristics on the growth and survival of Salmonella in beef tallow, white grease and poultry fat samples. Samples were inoculated with a high (~10⁸ CFU/mL) and a low (~10⁵ CFU/mL) Salmonella cocktail (S. Sentfenberg, S. Newport, S. Thompson and S. Infantis). Samples were stored for up to 5 days at 48 and 76 ºC. Remaining population was evaluated daily with and without enrichment step. Death rates were calculated using Weibull model for each temperature and moisture level. Only temperature had an effect (P &lt; 0.05) on Salmonella inactivation, while no effect between moisture and/or inoculum level were observed. When all products were challenged at 76 ˚C, counts were below detectable limits after 6 hours. At 48˚C a progressive decline in Salmonella population was observed within 3 days for both beef tallow and white grease when high inoculum was used for the challenge study. Salmonella was below detectable limit within 4 days for both fat types when a low inoculum was instead applied. This research identified the effect of moisture and temperature in rendered fat samples contaminated with Salmonella and underlined the need to use time-moisture-temperature data to minimize microbial growth during transportation and storage.

Energetic values of animal by-products for broiler chickens of different ages

The objective of this study was to determine the chemical composition and apparent metabolizable energy (AME), AME corrected for nitrogen balance (AMEn) and its respective metabolizable coefficients of animal byproducts for broiler chickens with different ages. Meat and bone meal, poultry by-product meal, tilapia processing residue and poultry fat were evaluated. A total of 760 male broiler chickens were used and evaluated and the phases: pre-starter (1 to 8 d of age); starter (11 to18 d); grower 1 (21 to 28 d); grower 2 (31 to 38 d), and finisher (41 to 48 d). Total excreta collection method was performed in five metabolism assays. The experimental design was completely randomized, and data were submitted to analysis of variance, posteriorly, the four feeds were compared by Tukey test and a regression analysis was performed with broiler chickens age. The significance was considered at 5% probability. The values of AME and AMEn were higher in older birds for all ingredients. The lesser CAME and CAMEn were obtained for meat and bone meal, for the regression analysis poultry by-product meal, tilapia processing residue and poultry fat had an increased linear effect with birds age and there was no adjust for meat and bone meal for regression analysis.

ASSESSMENT OF SAFETY AND FAT QUALITY OF BIRDS ‘CARCASES DURING THEIR PRODUCTION AND STORAGE ACCORDING TO DEVELOPED METHODS

The urgency of the work is the need to establish criteria for assessing the safety and quality of poultry meat at facilities for its production and storage, due to the development of new express and improved common methods of controlling the safety and quality of poultry meat for risks (biological, chemical, physical) their life cycle. Studies have assessed the safety and quality of poultry carcasses using new and improved methods for determining the acid and peroxide levels of fat compared to conventional methods for determining the degree of freshness of poultry meat. Organoleptic evaluation of fresh poultry meat was established – for storage in a refrigerated chamber at a temperature of 0−4 °С for 5 days; doubtful degree of freshness – birds for storage in a refrigerator at a temperature of 0−4 °C for 6–7 days; stale – for storage in a refrigerator at a temperature of 0−4 °C for more than 7 days on the appearance of the carcass, color, odor on the surface of the carcass and near the bones, the state of the thoracic cavity, subcutaneous and internal adipose tissue, muscle consistency, broth for cooking samples. There is a high probability of acid number of poultry fat of moderate freshness – 1.76±0.12 mg of NaOH (p≤0.001) and stale fat – 2.83±0.07 mg of NaOH (p≤0.001) compared to the acid number of fresh fat poultry (0.72±0.04 mg NaOH), as well as indicators of volatile fatty acids (VFA) in poultry meat of moderate degree of freshness – 6.62±0.43 mg KOH (p≤0.001) and stale fat – 11.05±0.37 mg KOH (p≤0.001) compared to the content of VFA of fresh poultry meat (2.61±0.24 mg KOH). Studies have established a high reliability of determining the indicators of volatile fatty acids in poultry meat – in 98.2–99.7 % and the results of studies on the indicators of the microscopic method for determining the number of bacteria in poultry meat – in 98.5–99.8 % compared to the acid number as determined by the developed method. The reliability of the indicators for determining the acid number of poultry fat was according to the developed method was 99.9 %. Therefore, the developed method for determining the acid number of poultry fat can be used to determine the quality of poultry meat, because the quality of meat depends on the quality of fat: the acid number of fresh poultry fat – up to 1.0 mg of NaOH; questionable degree of freshness – from 1.1 to 2.5 mg of NaOH; stale – more than 2.5 mg of NaOH. There is a high probability of peroxide content of poultry fat of the appropriate degree of freshness – 0.029 ± 0.002% J (p≤0.001) and stale fat – 0.063±0.003 % J (p≤0.001) compared to the peroxide value of fresh poultry fat (0.010±0.0007 % J), as well as indicators of volatile fatty acids (LFA) in poultry meat of moderate degree of freshness – 6.40±0.48 mg KOH (p≤0.001) and stale fat – 10.43±0.23 mg KOH (p≤0,001) compared to the VFA content of fresh poultry meat (2.58±0.23 mg KOH). The results showed that more reliable data compared to the results of studies for the determination of volatile fatty acids in poultry meat – 98.5–99.7 % and the results of studies on the indicators of the microscopic method for determining the number of bacteria in poultry meat – 99.0–99.6 % were obtained using the developed improved method. The reliability of the indicators for determining the peroxide value of poultry fat according to the developed improved method was 99.9 %. Therefore, the developed improved method for determining the peroxide value of poultry fat can be used to determine the quality of poultry meat, because the quality of meat depends on the quality of fat: peroxide value of fresh poultry fat – up to 0.010 % iodine of questionable degree of freshness – from 0.010 to 0.040 % iodine; stale – more than 0.040% iodine. The developed methods for determining the safety and quality of poultry carcass fat can be used by state veterinary inspectors as simple test methods to carry out appropriate state risk-based control of poultry meat production and storage facilities.

PSXI-32 Effects of algae DHA on fatty acid profile of plasma red blood cell membrane and fecal microbiota of adult cats

There is evidence that algae can be a sustainable alternative of omega-3 polyunsaturated fatty acids (w-3 PUFA; DHA and EPA) in the diets of felines, but more information is needed to determine bioavailability of algal w-3 PUFAs in felines. Therefore, the objective of this study was to determine the effects of dietary supplementation of algae DHA on plasma and red blood cell (RBC) membrane fatty acid profiles and fecal microbiota of adult cats. A complete randomized design was utilized with thirty female and male adult cats (mean age: 1.8 ± 0.03 yr, mean BW: 4.5 ± 0.8 kg) which were fed an assigned diet for 90 d. Three diets were formulated with poultry fat alone or inclusion of 2% fish oil or 2% algae DHA meal. Blood samples were collected after fasting on 0, 30, 60 and 90 d to be analyzed for plasma and red blood cell fatty acid profiles. A fresh fecal sample was collected within 15 min of defecation from each cat to be analyzed for fecal microbiota. Illumina 16S rRNA sequencing from V4 region was completed using MiSeq and analyzed using QIIME 2. Plasma and RBC fatty acid concentrations at baseline were similar among all cats and treatment groups. However, dietary treatment had a significant effect on the concentrations of several fatty acids in plasma and RBC over time. Plasma and RBC concentrations of DHA were greater (P &lt; 0.05) for cats fed the algal DHA diet compared to the control and fish oil diets. Conversely, plasma and RBC concentrations of EPA did not differ among treatments when analyzed as a change from baseline. Beta- and alpha-diversity did not differ among treatments, indicating that 2% fish oil or algal-DHA meal does alter fecal microbiota of cats in contrast with cats fed a poultry fat-based diet.

DHA from microalgae Schizochytrium spp. (Thraustochytriaceae) modifies the inflammatory response and gonadal lipid profile in domestic cats

The present study aimed to evaluate the inflammatory response, oxidative status and fatty acid deposition in reproductive tissues of cats supplemented with the dried microalgae Schizochytrium spp. (Thraustochytriaceae) as a DHA source. Thirty-seven cats (males, n 21; females, n 16; 11·5 (sd 0·5) months of age) were divided by sex into five groups. Treatment diets contained algae biomass at 4·0, 8·0, 12·0 or 16·0 g/kg replacing poultry fat (n-6 source). Cats were fed the respective diet for 62 d and neutered on day 58. Blood samples were collected at the beginning of the experiment (day 1), before neutering (day 58) and 4 d after surgery (day 62) for analysis of inflammation and oxidative markers. Acute-phase protein levels were altered (P < 0·01) in the postoperative period, without any treatment effect (P > 0·05). PGE2 concentrations after surgery were reduced linearly (R2 0·8706; P = 0·002) with microalgal inclusion. Blood platelet count was reduced (P = 0·001) after the surgery regardless treatment, but it was higher in the DHA group compared with control (P < 0·001). The DHA deposition (testicles, R2 0·846; ovaries, R2 0·869) and the n-6:n-3 ratio (testicles, R2 0·859; ovaries, R2 0·955) in gonads had a pattern which fitted a quadratic model. DHA from Schizochytrium spp. modifies PGE2 response after the surgery in cats. The physiological roles of the DHA in the reproduction of cats were not investigated, but its gonadal deposition after supplementation was observed.

Animal Fats in Rabbit Feeding – A Review

The purpose of this article is to overview the history of feeding rabbits with different types of animal fats, and to discuss their effects on rabbit performance and quality of their products. Other aspects of the inclusion of various animal fats in rabbit diets are also described. This article is based on the analysis of relevant scientific literature and presents animal fats fed to rabbits, such as beef tallow, butter, pork lard, poultry fat, fish oil, krill oil, oil extracted from insect larvae, mixtures of various animal fats, and mixtures of animal and vegetable fats. The reported papers describe the effect of fats on growth performance, lactation, rearing performance, meat quality, and health status of rabbits. It is notable that in many cases, various animal fats were often an integral part of numerous diets or were included in control diets. The presented information demonstrates that animal fat can be fed to rabbits at 2–4% of the diet without negative effects on reproductive performance, growth performance and quality of meat obtained. Rabbits were used as model animals in many studies in which fat was added to balance the diets and to increase their energy value, especially when investigating various cardiovascular and obesity-related diseases.