scholarly journals The Mi-EFF1/Minc17998 effector interacts with the soybean GmHub6 protein to promote host plant parasitism by Meloidogyne incognita

2021 ◽  
Vol 114 ◽  
pp. 101630
Author(s):  
Reneida Aparecida Godinho Mendes ◽  
Marcos Fernando Basso ◽  
Bruno Paes de Melo ◽  
Thuanne Pires Ribeiro ◽  
Rayane Nunes Lima ◽  
...  
Nematology ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 187-199 ◽  
Author(s):  
Miguel Talavera-Rubia ◽  
Alejandro Pérez De Luque ◽  
Manuel López-Gómez ◽  
Soledad Verdejo-Lucas

The development of Meloidogyne incognita and M. javanica on zucchini ‘Amalthee’ was compared to characterise critical events in plant parasitism. Meloidogyne incognita was much less successful parasitising zucchini than M. javanica despite similarities in penetration rates and juvenile development. The increased frequency of undersized individuals, immature females and empty galls evidenced a failure in M. incognita development. Meloidogyne incognita induced larger feeding sites that contained more and larger giant cells than did M. javanica. Malformation of the M. incognita giant cells and abnormal growth of the surrounding tissues was observed at both 11 and 25 days post-inoculation. Critical events in parasitism differentiating the nematode isolates were the transition from fourth-stage juveniles to females, and the reduced fertility of the egg-laying females. Zucchini can be considered a source of resistance to M. incognita because it restricted nematode proliferation by supporting less fertile egg-laying females and producing fewer egg masses and total eggs.


2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Qianqian Shi ◽  
Zhenchuan Mao ◽  
Xi Zhang ◽  
Xiaoping Zhang ◽  
Yunsheng Wang ◽  
...  

2020 ◽  
Author(s):  
Reneida Aparecida Godinho Mendes ◽  
Marcos Fernando Basso ◽  
Bruno Paes de Melo ◽  
Rayane Nunes Lima ◽  
Janaina Fernandes de Araújo ◽  
...  

Abstract Background: Meloidogyne incognita is the most frequently reported species from the root-knot nematode (RKN) complex responsible for causing damage in several different crops worldwide. The interaction between M. incognita and host plants involves the secretions of molecular factors from the nematode, which mainly suppress the defense response and promote plant parasitism. On the other hand, several plant elements are associated with the immune defense system that opposes nematode infection.Results: In this study, the interaction of the Mi-EFF1/Minc17998 effector with the soybean GmHub6 (Glyma.17G099100; TCP14) protein was identified and characterized in vitro and in vivo. Data showed that the GmHub6 gene is upregulated by M. incognita infection in a nematode-resistant soybean cultivar (PI595099) compared to a susceptible cultivar (BRS133). Accordingly, the Arabidopsis thaliana AtHub6 mutant line (AT3G47620, orthologous gene of GmHub6 displayed normal vegetative development of the plant but was more susceptible to M. incognita. Thus, since the soybean and A. thaliana Hub6 proteins are TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factors involved in plant development and morphogenesis modulation, flowering time regulation, and the activation of the plant immune system, our data suggest that the interaction of Mi-EFF1/Minc17998 and Hub6 proteins is associated with an increase in plant susceptibility to nematode infection during parasitism. It is suggested that this interaction may prevent the nuclear localization or disturb the activity of GmHub6 as a typical transcription factor modulating the cell cycle of the plant, avoid the activation of the host’s defense response, and successfully promote parasitism.Conclusion: Our findings indicate the potential of the Mi-EFF1/Minc17998 effector for the development of biotechnological tools based on the approaches of RNA interference and GmHub6 gene overexpression for RKN control.


2020 ◽  
Author(s):  
Reneida Aparecida Godinho Mendes ◽  
Marcos Fernando Basso ◽  
Bruno Paes de Melo ◽  
Rayane Nunes Lima ◽  
Janaina Fernandes de Araújo ◽  
...  

Abstract Background: Meloidogyne incognita is the most frequently reported species from the root-knot nematode (RKN) complex responsible for causing damage in several different crops worldwide. The interaction between M. incognita and host plants involves the secretions of molecular factors from the nematode, which mainly suppress the defense response and promote plant parasitism. On the other hand, several plant elements are associated with the immune defense system that opposes nematode infection. Results: In this study, the interaction of the Mi-EFF1/Minc17998 effector with the soybean GmHub6 (Glyma.17G099100; TCP14) protein was identified and characterized in vivo and in planta. Data showed that the GmHub6 gene is upregulated by M. incognita infection in a nematode-resistant soybean cultivar (PI595099) compared to a susceptible cultivar (BRS133). Accordingly, the Arabidopsis thaliana AtHub6 mutant line (AT3G47620, orthologous gene of GmHub6 displayed normal vegetative development of the plant but was more susceptible to M. incognita. Thus, since the soybean and A. thaliana Hub6 proteins are TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factors involved in plant development and morphogenesis modulation, flowering time regulation, and the activation of the plant immune system, our data suggest that the interaction of Mi-EFF1/Minc17998 and Hub6 proteins is associated with an increase in plant susceptibility to nematode infection during parasitism. It is suggested that this interaction may prevent the nuclear localization or disturb the activity of GmHub6 as a typical transcription factor modulating the cell cycle of the plant, avoid the activation of the host’s defense response, and successfully promote parasitism. Conclusion: Our findings indicate the potential of the Mi-EFF1/Minc17998 effector for the development of biotechnological tools based on the approaches of RNA interference and GmHub6 gene overexpression for RKN control.


Author(s):  
C. Sankar ◽  
K. Soorianathasundaram ◽  
N. Kumar ◽  
G. Karunakaran ◽  
M. Sivakumar

The reaction of twenty four new synthetic banana hybrids were selected and evaluated for resistance to M. incognita under artificially inoculated pot conditions. Known susceptible (Grand Naine) and resistant (Yankambi KM5) banana varieties were included as reference cultivars. Responses of banana hybrids to M. incognita infection was assessed by the nematodes population in roots, number of galled roots, percentage of galled roots and root gall index on a scale of 1-5, where 0 = 0 galls and 5≥100 galls. The result showed that seven banana hybrids, H 904, H 911, H  921, H 924, H 926, H 943 and H 952 were found to be tolerance to M. incognita and the remaining were rated as moderate susceptible and susceptible. The total phenols, peroxidase, polyphenol oxidase, phenylalanine ammonia lyase chemical and enzyme contains in roots were higher in tolerance genotypes viz-a-vis susceptible ones.


Fruits ◽  
2009 ◽  
Vol 64 (5) ◽  
pp. 295-303 ◽  
Author(s):  
Hang Ye ◽  
Wen-jun Wang ◽  
Guo-jie Liu ◽  
Li-xin Zhu ◽  
Ke-gong Jia

ENTOMON ◽  
2020 ◽  
Vol 45 (3) ◽  
pp. 237-238
Author(s):  
J. Nayanathara ◽  
R. Narayana
Keyword(s):  
New Host ◽  

Anthene lycaenina lycaenina (R. Felder, 1868) is reported on mango for the first time.


2019 ◽  
Vol 16 (1) ◽  
pp. 12-15
Author(s):  
M.D. Zerova ◽  
A. Al-Sendi ◽  
V.N. Fursov ◽  
H. Adeli-Manesh ◽  
S.E. Sadeghi ◽  
...  

The new species, Bruchophagus ayadi sp.n., is reared from seed pods of Melilotus officinalis (L.) Desr. (Fabaceae) in Iran (Lorestan). The new species is close to B. platypterus (Walk.), but differs by roundish abdomen and very gibbous, almost globular (in lateral view) mesosoma. These species can be also differentiated by some biological features. The host plant of B. platypterus is Lotus corniculatus L., whereas the new species is reared from Melilotus officinalis (L.) Desr. Holotype of Bruchophagus ayadi sp.n. is deposited in the collection of I.I. Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine (Kyiv).


Sign in / Sign up

Export Citation Format

Share Document