Evaluation of a milk ELISA as an alternative to a serum ELISA in the determination of the prevalence and incidence of brucellosis in dairy herds in Hubei Province, China

2020 ◽  
Vol 182 ◽  
pp. 105086
Author(s):  
Yu Wang ◽  
Ian D Robertson ◽  
Shuang Cheng ◽  
Yan Wang ◽  
Liyue Hou ◽  
...  
Keyword(s):  
Hubei Province ◽  
Dairy Herds ◽  
Milk Elisa ◽  
Serum Elisa

scholarly journals Comparative Evaluation of Different Test Combinations for Diagnosis ofMycobacterium aviumSubspeciesparatuberculosisInfecting Dairy Herds in India

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Rajni Garg ◽  
Prasanna Kumar Patil ◽  
Shoor Vir Singh ◽  
Shukriti Sharma ◽  
Ravi Kumar Gandham ◽  
...  
Keyword(s):  
Relative Sensitivity ◽  
Screening Tests ◽  
Confirmatory Test ◽  
Dairy Herds ◽  
Milk Samples ◽  
Milk Elisa ◽  
Cattle Herds ◽  
Lactating Dairy Cattle ◽  
Serum Elisa ◽  
Screening And Diagnosis

A total of 355 cows were sampled (serum,n=315; faeces,n=355; milk,n=209) from dairy farms located in the Punjab state of India. Faeces and serum/milk samples were screened by acid fast staining and “indigenous ELISA,” respectively. IS900PCR was used to screen faeces and milk samples. Bio-load of MAP in dairy cows was 36.9, 15.6, 16.3, and 14.4%, using microscopy, serum ELISA, milk ELISA and milk PCR, respectively. Estimated kappa values between different test combinations: serum and milk ELISA, faecal microscopy and faecal PCR, milk ELISA and milk PCR, faecal PCR and serum ELISA were 0.325, 0.241, 0.682, and 0.677, respectively. Estimation of the relative sensitivity and specificity of different tests in the present study indicated that “serum ELISA” and “milk ELISA” were good screening tests, add “milk PCR” was “confirmatory test” for MAP infection. Combination of milk ELISA with milk PCR may be adopted as a model strategy for screening and diagnosis of JD in lactating/dairy cattle herds in Indian conditions.

scholarly journals Determining the Prevalence and Seasonality of Fasciola hepatica in Pasture-based Dairy herds in Ireland using a Bulk Tank Milk ELISA

2015 ◽  
Vol 68 (1) ◽  
Author(s):  
Yris Bloemhoff ◽  
Andrew Forbes ◽  
Martin Danaher ◽  
Barbara Good ◽  
Eric Morgan ◽  
...  
Keyword(s):  
Fasciola Hepatica ◽  
Dairy Herds ◽  
Bulk Tank Milk ◽  
Milk Elisa ◽  
Bulk Tank

scholarly journals The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the CzechRepublic: a case report

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 549-552 ◽  
Author(s):  
L. Hurkova ◽  
D. Halova ◽  
D. Modry
Keyword(s):  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Elisa Test ◽  
Rapid Screening ◽  
Dairy Herds ◽  
Caninum Infection ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Bovine Abortion ◽  
Bulk Milk Elisa

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk milk ELISA could be a useful and inexpensive method for rapid screening of Neospora caninum infection in dairy herds in large areas.

Seasonal variation of Fasciola hepatica antibodies in dairy herds in Northern Ireland measured by bulk tank milk ELISA

2018 ◽  
Vol 117 (9) ◽  
pp. 2725-2733 ◽  
Author(s):  
Andrew W. Byrne ◽  
Jordon Graham ◽  
James McConville ◽  
Georgina Milne ◽  
Stanley McDowell ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Northern Ireland ◽  
Fasciola Hepatica ◽  
Dairy Herds ◽  
Bulk Tank Milk ◽  
Milk Elisa ◽  
Bulk Tank

scholarly journals Determination of presence of Tritrichomonas foetus in uterine lavages from cows with reproductive problems

2012 ◽  
Vol 21 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Lady Carolina González-Carmona ◽  
Milena Jineth Sánchez-Ladino ◽  
Rubiela Castañeda-Salazar ◽  
Adriana del Pilar Pulido-Villamarín ◽  
Humberto Guáqueta-Munar ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Basal Medium ◽  
Physiological Saline ◽  
Dark Field ◽  
Dairy Herds ◽  
Significant Finding ◽  
Tritrichomonas Foetus ◽  
Physiological Saline Solution ◽  
History Of

The aim of this study was to determine the presence of Tritrichomonasfoetus in two dairy herds on the Altiplano Cundiboyacense. Twenty-one low-volume uterine lavages from cows with a history of reproductive problems in two dairy herds located in the municipality of Sibaté (Cundinamarca) and Ventaquemada (Boyacá) were evaluated. In the first herd, 10 cows were sampled and in the second, 11 cows, based on three inclusion criteria. The uterine lavages were obtained through infusion of physiological saline solution into the uterine body. The samples were centrifuged and seeded in Tritrichomonas basal medium for 10-15 days at 37 ºC. The protozoa were evaluated on the day of sampling and 10 and 15 days after incubation by means of direct viewing under a dark-field microscope. Positive samples were stained with Wright and Lugol to identify the morphological characteristics. This study showed that T. foetus was present in 61.8% of the animals sampled. The determination that T. foetus was present in 61.8% of the samples analyzed is a significant finding given that in the herds evaluated, this agent had not previously been diagnosed.

scholarly journals Prevalence and etiological agents of subclinical mastitis at the end of lactation in nine dairy herds in North-East Poland

2016 ◽  
Vol 19 (1) ◽  
pp. 119-124 ◽  
Author(s):  
M. Sztachańska ◽  
W. Barański ◽  
T. Janowski ◽  
J. Pogorzelska ◽  
S. Zduńczyk
Keyword(s):  
Somatic Cell Count ◽  
Fungal Infections ◽  
Subclinical Mastitis ◽  
Dairy Herds ◽  
Bacteriological Examination ◽  
Coagulase Negative Staphylococci ◽  
Staph Aureus ◽  
North East ◽  
Etiological Agents

AbstractThe aim of this study was to evaluate the prevalence and etiological agents of subclinical mastitis at the end of lactation in nine dairy herds in North-East Poland. In total, 387 Polish HF were involved in the study. The diagnosis of mastitis was performed on the basis of clinical examination of the udder, macroscopic evaluation of milk, determination of somatic cell count and bacteriological examination of milk. Subclinical mastitis was found in an average of 36.7% (range from 21.0% to 53.1%) of cows and of 15.7% (range from 9.6% to 25.2%) of quarters. Coagulase negative staphylococci (CNS; 31.6% of quarters), Streptococcus (Str.) agalactiae (15.6% of quarters), Staphylococcus (Staph.) aureus (12.1% of quarters) and fungi (12.2% of quarters) were most frequently isolated from subclinical mastitis. Etiological agents of subclinical mastitis differed strongly between herds. The results of this study showed that the incidence of subclinical mastitis at the end of lactation in dairy herds in North-East Poland is high. CNS were the most frequently isolated from subclinical mastitis cases, however mastitis caused by the contagious pathogens Str. agalactiae and Staph. aureus is still a problem. The fungal infections of the mammary gland also play an important role.

scholarly journals Using a Herd Profile to Determine Age-Specific Prevalence of Bovine Leukemia Virus in Michigan Dairy Herds

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Ronald J. Erskine ◽  
Paul C. Bartlett ◽  
Todd M. Byrem ◽  
Chelsea L. Render ◽  
Catherine Febvay ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Malignant Neoplasm ◽  
Dairy Herd ◽  
Dairy Herds ◽  
Management Plans ◽  
Milk Elisa ◽  
The Mean ◽  
Bovine Leukosis ◽  
Simple Average

Enzootic bovine leukosis is a contagious disease of cattle caused by the retrovirus, bovine leukemia virus (BLV) and is the most common cause of malignant neoplasm in cattle. In order to facilitate surveillance of this disease in dairy herds, we developed a method to combine ELISA of milk collected during routine production testing with a prescribed sampling of cows that is independent of the proportion of cows within each lactation. In 113 Michigan dairy herds, milk samples from ten cows in each of the 1st, 2nd, 3rd, and ≥4th lactations were analyzed for anti-Bovine Leukemia Virus (BLV) antibodies by milk ELISA. For each herd, a BLV herd profile (BHP) was calculated as the simple average of the percent of BLV-positive cows within each of the four lactation groups. The mean BHP for all herds was 32.8%, with means of 18.5, 28.8, 39.2, and 44.8% of 1st, 2nd, 3rd, and ≥4th lactation animals infected, respectively. In eight herds, we determined the correlation between the BHP, and true herd prevalence by testing the entire lactating herd (r=0.988,  P<0.0001). The BHP allows discrimination of lactation-specific BLV prevalence within a dairy herd, to help identify risk factors and management plans that may be important in transmission of BLV.

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