Directed immobilization of CGTase: The effect of the enzyme orientation on the enzyme activity and its use in packed-bed reactor for continuous production of cyclodextrins

An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in atert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1 mL/min, temperature52.1∘C, and substrate molar ratio 1 : 4. The predicted and experimental values of molar conversion were83.31±2.07% and82.81±.98%, respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis.

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Effectiveness of Adjustable-Volume Packed-Bed Reactor with an Ion-Exchange Resin Catalyst for Continuous Production

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN ◽

10.1252/jcej.15we243 ◽

2016 ◽

Vol 49 (7) ◽

pp. 668-672 ◽

Cited By ~ 5

Author(s):

Kota Yamazaki ◽

Naomi Shibasaki-Kitakawa ◽

Kazunori Nakashima ◽

Toshikuni Yonemoto

Keyword(s):

Ion Exchange ◽

Exchange Resin ◽

Continuous Production ◽

Packed Bed ◽

Ion Exchange Resin ◽

Packed Bed Reactor ◽

Resin Catalyst

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Lipase Acrylic Resin Catalyzed Interesterification of Sewage Sludge in Micro Packed Bed Reactor: Box-Behnken Design

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.870.81 ◽

2020 ◽

Vol 870 ◽

pp. 81-96

Author(s):

Ali A. Jazie ◽

Riyam Imad Jaddan ◽

Mohamed F. Al-Dawody ◽

Suhad A. Abed

Keyword(s):

Enzyme Activity ◽

Sewage Sludge ◽

Ethyl Acetate ◽

Acrylic Resin ◽

Packed Bed ◽

Biodiesel Production ◽

Packed Bed Reactor ◽

Heating Process ◽

Optimum Conditions ◽

Box Behnken Design

Ethyl acetate as acyl accepter have been used for sewage sludge biodiesel production in micro packed bed reactor. Lipase acrylic resin enzyme activity was investigated in the interesterification reaction of converting the sewage sludge into a biodiesel product. The main parameters affecting the process was adjusted via the design of Box-Behnken and approach of response surface then the optimum conditions were as follow (reaction time=15 h, ethyl acetate/oil ratio=24:1, temperature=40 °C and enzyme quantity=18 wt%) providing 95.78 % biodiesel yield. The lipase acrylic resin was examined under the optimum conditions for the reusability. the flow effect and the heating process of the micro packed bed reactor was investigated. The product biodiesel physicochemical properties were evaluated according to the ASTM D-6751-2 and found acceptable comparable to the mineral diesel properties.

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Continuous production of xylooligosaccharides in a packed bed reactor with immobilized–stabilized biocatalysts of xylanase from Aspergillus versicolor

Journal of Molecular Catalysis B Enzymatic ◽

10.1016/j.molcatb.2013.09.017 ◽

2013 ◽

Vol 98 ◽

pp. 8-14 ◽

Cited By ~ 19

Author(s):

Caio C. Aragon ◽

Andrea F. Santos ◽

Ana I. Ruiz-Matute ◽

Nieves Corzo ◽

Jose M. Guisan ◽

...

Keyword(s):

Continuous Production ◽

Packed Bed ◽

Packed Bed Reactor ◽

Aspergillus Versicolor

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Continuous production of ellagic acid in a packed-bed reactor

Process Biochemistry ◽

10.1016/j.procbio.2014.06.005 ◽

2014 ◽

Vol 49 (10) ◽

pp. 1595-1600 ◽

Cited By ~ 9

Author(s):

Juan Buenrostro-Figueroa ◽

Sergio Huerta-Ochoa ◽

Arely Prado-Barragán ◽

Juan Ascacio-Valdés ◽

Leonardo Sepúlveda ◽

...

Keyword(s):

Ellagic Acid ◽

Continuous Production ◽

Packed Bed ◽

Packed Bed Reactor

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Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.681253 ◽

2021 ◽

Vol 9 ◽

Author(s):

Mi Na Choi ◽

Kyung-Chul Shin ◽

Dae Wook Kim ◽

Baek-Joong Kim ◽

Chang-Su Park ◽

...

Keyword(s):

Continuous Production ◽

Nucleoside Analogs ◽

Packed Bed ◽

Glucose Isomerase ◽

Industrial Applications ◽

Packed Bed Reactor ◽

Rare Sugar ◽

Potential Applications ◽

Rare Sugars ◽

Immobilized Glucose Isomerase

Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D-allose production were determined to be pH 8.0 and 60°C, with 500 g/L D-allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D-allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D-allose from D-allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D-allose.

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