scholarly journals Major histocompatibility complex B variability in Korean native chicken breeds

2020 ◽  
Vol 99 (10) ◽  
pp. 4704-4713 ◽  
Author(s):  
Prabuddha Manjula ◽  
Janet E. Fulton ◽  
Dongwon Seo ◽  
Jun Heon Lee
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Native Chicken ◽  
Chicken Breeds ◽  
Korean Native Chicken

Genetic diversity analysis of the major histocompatibility complex (MHC) region in Indian native chicken breeds and pureline chickensusing the LEI0258 microsatellite marker

2017 ◽  
Author(s):  
T R Kannak ◽  
M. R. Reddy ◽  
K. S. Raja ravindra ◽  
R. N. Chatterjee
Keyword(s):  
Major Histocompatibility Complex ◽  
Microsatellite Marker ◽  
Major Histocompatibility ◽  
Breeding Programs ◽  
Histocompatibility Complex ◽  
Native Chicken ◽  
Chicken Breeds ◽  
Genetic Resource Conservation ◽  
Chicken Major Histocompatibility Complex ◽  
Two Populations

The chicken major histocompatibility complex (MHC) plays an important role in immune response and is strongly associated with resistance and susceptibility to many diseases. The microsatellite marker LEI0258 is physically located within MHC, between the BG and BF regions and can identify serologically well-defined MHC haplotypes. In the present study, a total of 150 samples from pureline chicken breeds including Indian native chicken breeds Aseel (20), Kadaknath (45), White Leghorn (IWI-20; IWK-23), Dahlem red (DR-20), Broiler (PB1-22) was investigated by using LEI0258 microsatellite marker. Overall 22 differently sized alleles (182-552 bp) were identified in all the five lines. Our results indicate that Indian native chicken breeds Aseel (Na=10) and Kadaknath (Na=11) harbored more alleles compared to those of pureline populations (Na=5-6). Analysis of Hardy-Weinberg’s equilibrium (HWE) revealed that two populations IWI and Kadaknath deviated from the HWE (P less than 0.001). LEI0258 microsatellite based MHC haplotyping would be useful in selecting birds for breeding programs and also in genetic resource conservation.

scholarly journals Genetic diversity at major histocompatibility complex and its effect on production and immune traits in indigenous chicken breeds of India

2020 ◽  
Vol 63 (1) ◽  
pp. 173-182
Author(s):  
Santosh Haunshi ◽  
Divya Devara ◽  
Kannaki Ramasamy ◽  
Rajkumar Ullengala ◽  
Rudra Nath Chatterjee
Keyword(s):  
Genetic Diversity ◽  
Major Histocompatibility Complex ◽  
Egg Production ◽  
Major Histocompatibility ◽  
Indigenous Chicken ◽  
Histocompatibility Complex ◽  
Antibody Titres ◽  
Chicken Breeds ◽  
Immune Traits ◽  
Mhc Alleles

Abstract. The genetic diversity at major histocompatibility complex (MHC) in indigenous chicken breeds of India (Ghagus and Nicobari) in comparison with the White Leghorn (WLH) breed was investigated by genotyping the MHC-linked LEI0258 marker. Altogether 38 alleles and 96 genotypes were observed among three breeds. The observed and effective alleles were highest in Ghagus (23, 8.3) followed by Nicobari (14, 3.2) and WLH (10 and 2.2) breeds. The size of alleles ranged from 193 to 489 bp in Ghagus, 193 to 552 bp in Nicobari and 241 to 565 bp in the WLH breed. The number of private alleles was also highest in Ghagus (18) followed by Nicobari (8) and WLH (5) breeds. The most frequent allele was 261 bp in WLH (66 %), 343 bp in Nicobari (50.4 %) and 309 bp in the Ghagus (28.15 %) breed. Observed and expected heterozygosities were highest in Ghagus (0.83, 0.88) followed by Nicobari (0.58, 0.68) and WLH (0.53, 0.54). The genetic distance (Nei) between Ghagus and Nicobari breeds (2.24) was higher as compared to that of Ghagus and WLH (1.23) and that between Nicobari and WLH breeds (0.89). Association analysis revealed significant influence of MHC alleles on body weight, egg production in Ghagus and WLH breeds and antibody titres to Newcastle disease vaccine in the Nicobari breed.

Relationship of Major Histocompatibility Complex Class II Genes to Inhibitor Antibody Formation in Hemophilia A

1990 ◽  
Vol 64 (04) ◽  
pp. 564-568 ◽  
Author(s):  
Lloyd E Lippert ◽  
Lyman Mc A Fisher ◽  
Lawrence B Schook
Keyword(s):  
Major Histocompatibility Complex ◽  
Factor Viii ◽  
Antibody Formation ◽  
Rflp Analysis ◽  
Class Ii ◽  
Major Histocompatibility ◽  
Odds Ratios ◽  
Inhibitory Antibody ◽  
Histocompatibility Complex ◽  
Hla Dr

SummaryApproximately 14% of transfused hemophiliacs develop an anti-factor VIII inhibitory antibody which specifically neutralizes factor VIII procoagulant activity. In this study an association of the major histocompatibility complex (MHC) with inhibitor antibody formation was evaluated by restriction fragment length polymorphism (RFLP) analysis using BamHI, EcoRI, HindII, PstI, PvuII and TaqI digested genomic DNA probed with DP beta, DQ alpha, DQ beta and DR beta class II MHC gene probes. The RFLP patterns for 16 non-inhibitor and 11 inhibitor hemophiliac patients were analyzed. These 24 enzyme:probe combinations generated 231 fragments. Fifteen (15) fragments associated with the inhibitor phenotype; odds ratios ranged from 5.1 to 45 and lower bounds of 95% confidence intervals were > 1.000 for all 15 fragments. Five (5) fragments associated with non-inhibitors, with odds ratios ranging from 6.4 to 51.7. This report establishes a MHC related genetic basis for the inhibitor phenotype. No statistically significant differences in the distribution of serologically defined HLA-DR phenotypes were observed between the inhibitor and non-inhibitor groups.

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