Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

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Process optimization for deep eutectic solvent pretreatment and enzymatic hydrolysis of sugar cane bagasse for cellulosic ethanol fermentation

Renewable Energy ◽

10.1016/j.renene.2021.05.131 ◽

2021 ◽

Author(s):

Yao Liu ◽

Xiaojie Zheng ◽

Shunhui Tao ◽

Lei Hu ◽

Xiaodong Zhang ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Process Optimization ◽

Sugar Cane ◽

Ethanol Fermentation ◽

Cellulosic Ethanol ◽

Deep Eutectic Solvent ◽

Sugar Cane Bagasse ◽

Hydrolysis Of

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Efficient ethanol production from dried oil palm trunk treated by hydrothermolysis and subsequent enzymatic hydrolysis

Biotechnology for Biofuels ◽

10.1186/s13068-015-0263-6 ◽

2015 ◽

Vol 8 (1) ◽

Cited By ~ 12

Author(s):

In-Yong Eom ◽

Ju-Hyun Yu ◽

Chan-Duck Jung ◽

Kyung-Sik Hong

Keyword(s):

Enzymatic Hydrolysis ◽

Ethanol Production ◽

Oil Palm ◽

Oil Palm Trunk

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Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/206/1/012008 ◽

2017 ◽

Vol 206 ◽

pp. 012008

Author(s):

F S Hashim ◽

H W Yussof ◽

M A K M Zahari ◽

R A Rahman ◽

R M Illias

Keyword(s):

Enzymatic Hydrolysis ◽

Oil Palm ◽

Oil Palm Frond ◽

Hydrolysis Of ◽

Palm Frond

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Fermentative l-lactic acid production from pretreated whole slurry of oil palm trunk treated by hydrothermolysis and subsequent enzymatic hydrolysis

Bioresource Technology ◽

10.1016/j.biortech.2015.02.060 ◽

2015 ◽

Vol 185 ◽

pp. 143-149 ◽

Cited By ~ 23

Author(s):

In-Yong Eom ◽

Young-Hoon Oh ◽

Si Jae Park ◽

Seung-Hwan Lee ◽

Ju-Hyun Yu

Keyword(s):

Lactic Acid ◽

Enzymatic Hydrolysis ◽

Oil Palm ◽

Acid Production ◽

Lactic Acid Production ◽

Oil Palm Trunk ◽

Whole Slurry

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Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya

E-Journal Menara Perkebunan ◽

10.22302/ppbbi.jur.mp.v83i1.12 ◽

2016 ◽

Vol 83 (1) ◽

Author(s):

Vera BARLIANTI ◽

Deliana DAHNUM ◽

. MURYANTO ◽

Eka TRIWAHYUNI ◽

Yosi ARISTIAWAN ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Oil Palm ◽

Volume Ratio ◽

Enzyme Concentration ◽

Reducing Sugar ◽

Economic Feasibility ◽

First Order ◽

Empty Fruit Bunches ◽

Hydrolysis Process ◽

Hydrolysis Of

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi. Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung. Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu. Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The maximum sugar concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.

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