Role of bacterial cell surface structures in Escherichia coli biofilm formation

2005 ◽  
Vol 156 (5-6) ◽  
pp. 626-633 ◽  
Author(s):  
Rob Van Houdt ◽  
Chris W. Michiels
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Biofilm Formation ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures

scholarly journals Role of Cell Surface Structures in Biofilm Formation by <i>Escherichia coli</i>

2015 ◽  
Vol 06 (12) ◽  
pp. 1160-1165 ◽  
Author(s):  
Hafida Zahir ◽  
Hamadi Fatima ◽  
Lekchiri Souad ◽  
Mliji El Mostafa ◽  
Ellouali Mostafa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Biofilm Formation ◽  
Surface Structures ◽  
Cell Surface Structures

scholarly journals Phosphoethanolamine cellulose enhances curli-mediated adhesion of uropathogenicEscherichia colito bladder epithelial cells

2018 ◽  
Vol 115 (40) ◽  
pp. 10106-10111 ◽  
Author(s):  
Emily C. Hollenbeck ◽  
Alexandra Antonoplis ◽  
Chew Chai ◽  
Wiriya Thongsomboon ◽  
Gerald G. Fuller ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cell Monolayer ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures ◽  
Bladder Cell ◽  
Type 1 Pili ◽  
Tract Infections

UropathogenicEscherichia coli(UPEC) are the major causative agents of urinary tract infections, employing numerous molecular strategies to contribute to adhesion, colonization, and persistence in the bladder niche. Identifying strategies to prevent adhesion and colonization is a promising approach to inhibit bacterial pathogenesis and to help preserve the efficacy of available antibiotics. This approach requires an improved understanding of the molecular determinants of adhesion to the bladder urothelium. We designed experiments using a custom-built live cell monolayer rheometer (LCMR) to quantitatively measure individual and combined contributions of bacterial cell surface structures [type 1 pili, curli, and phosphoethanolamine (pEtN) cellulose] to bladder cell adhesion. Using the UPEC strain UTI89, isogenic mutants, and controlled conditions for the differential production of cell surface structures, we discovered that curli can promote stronger adhesive interactions with bladder cells than type 1 pili. Moreover, the coproduction of curli and pEtN cellulose enhanced adhesion. The LCMR enables the evaluation of adhesion under high-shear conditions to reveal this role for pEtN cellulose which escaped detection using conventional tissue culture adhesion assays. Together with complementary biochemical experiments, the results support a model wherein cellulose serves a mortar-like function to promote curli association with and around the bacterial cell surface, resulting in increased bacterial adhesion strength at the bladder cell surface.

Bacterial Cell Surface Structures in Yersinia enterocolitica

2012 ◽  
Vol 60 (3) ◽  
pp. 199-209 ◽  
Author(s):  
Nataniel Białas ◽  
Katarzyna Kasperkiewicz ◽  
Joanna Radziejewska-Lebrecht ◽  
Mikael Skurnik
Keyword(s):  
Cell Surface ◽  
Yersinia Enterocolitica ◽  
Bacterial Cell ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures

Functions of Bacterial Cell Surface Structures

BioScience ◽  
1985 ◽  
Vol 35 (3) ◽  
pp. 172-177 ◽  
Author(s):  
F. G. Ferris ◽  
T. J. Beveridge
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Structures ◽  
Bacterial Cell Surface ◽  
Cell Surface Structures
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