Ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes

2006 ◽  
Vol 157 (4) ◽  
pp. 376-385 ◽  
Author(s):  
Spyridon Ntougias ◽  
Georgios I. Zervakis ◽  
Constantinos Ehaliotis ◽  
Nektarios Kavroulakis ◽  
Kalliope K. Papadopoulou
2012 ◽  
pp. 347-354 ◽  
Author(s):  
R. García-de-la-Fuente ◽  
D. Mendoza-Hernández ◽  
F. Fornes ◽  
R.M. Belda ◽  
J. Girbent ◽  
...  

2006 ◽  
Vol 41 (3) ◽  
pp. 616-623 ◽  
Author(s):  
M.L. Cayuela ◽  
M.A. Sánchez-Monedero ◽  
A. Roig

Author(s):  
Rocío Reina ◽  
Mercedes García-Sánchez ◽  
Christiane Liers ◽  
Inmaculada García-Romera ◽  
Elisabet Aranda

2010 ◽  
Vol 76 (19) ◽  
pp. 6564-6571 ◽  
Author(s):  
Marta Goberna ◽  
Maria Gadermaier ◽  
Carlos García ◽  
Bernhard Wett ◽  
Heribert Insam

ABSTRACT The acclimatization of methanogens to two-phase olive mill wastes (TPOMW) was investigated in pilot fermenters started up with cattle excreta (37°C) and after changing their feed to excreta plus TPOMW (37°C or 55°C) or TPOMW alone (37°C) until a steady state was reached (28 days). Methanogenic diversity was screened using a phylogenetic microarray (AnaeroChip), and positive targets were quantified by real-time PCR. Results revealed high phylogenetic richness, with representatives of three out of the four taxonomic orders found in digesters. Methanosarcina dominated in the starting excreta (>96% of total 16S rRNA gene copies; over 45 times more abundant than any other methanogen) at high acetate (0.21 g liter−1) and ammonia N concentrations (1.3 g liter−1). Codigestion at 37°C induced a 6-fold increase of Methanosarcina numbers, correlated with CH4 production (r Pearson = 0.94; P = 0.02). At 55°C, the rise in temperature and H2 partial pressure induced a burst of Methanobacterium, Methanoculleus, Methanothermobacter, and a group of uncultured archaea. The digestion of excreta alone resulted in low but constant biogas production despite certain oscillations in the methanogenic biomass. Unsuccessful digestion of TPOMW alone was attributed to high Cu levels inducing inhibition of methanogenic activity. In conclusion, the versatile Methanosarcina immediately adapted to the shift from excreta to excreta plus TPOMW and was responsible for the stimulated CH4 production at 37°C. Higher temperatures (55°C) fostered methanogenic diversity by promoting some H2 scavengers while yielding the highest CH4 production. Further testing is needed to find out whether there is a link between increased methanogenic diversity and reactor productivity.


Chemosphere ◽  
2010 ◽  
Vol 81 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Miguel A. Sánchez-Monedero ◽  
Nuria Serramiá ◽  
Concepción García-Ortiz Civantos ◽  
Antonia Fernández-Hernández ◽  
Asunción Roig

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5790
Author(s):  
M. Mercè Solé ◽  
Laia Pons ◽  
Mireia Conde ◽  
Carmen Gaidau ◽  
Anna Bacardit

Olive mill wastes represent an important environmental problem. Their high phenol, lipid, and organic acid concentrations turn them into phytotoxic materials. Specifically, wet olive pomace (WOP) is the waste generated in the two-phase continuous extraction process. WOP is a paste with around 60% water. The total volume of WOP generated is around 0.25 L/kg of olives processed. Its current waste management practices result in environmental problems as soil contamination, underground seepage, water-bodies pollution, and foul odor emissions. Some valorization alternatives include composting, biological treatments, direct combustion for energy production, or direct land application. The leather industry is making great efforts to apply cleaner processes while substituting chemical products for natural products. In this way, different alternatives are being studied, such as the use of zeolites, triazine derivatives, grape seed extract, olive leaf extract, etc. In this work, the use of wet olive pomace is presented as a possible alternative to conventional vegetable tannins (mimosa, quebracho, chestnut, etc.). Although different projects and studies have been developed for the valorization of olive mill wastes, there is completely a new approach to the WOP application for tanning purposes. This study shows that WOP has a significant number of polyphenolic substances, so it has a great potential to be used as a tanning agent. Specifically, this study has been able to determine that, of the polyphenols present in WOP, 39.6% correspond to tannins that are capable of tanning the skin. Additionally, it contains 14.3% non-tannins, that is, molecules that by themselves do not have the capacity to tan the leather but promote the tanning mechanism and improve the properties of the tanned leather.


2010 ◽  
Vol 30 (10) ◽  
pp. 1939-1947 ◽  
Author(s):  
N. Serramiá ◽  
M.A. Sánchez-Monedero ◽  
A. Fernández-Hernández ◽  
C. García-Ortiz Civantos ◽  
A. Roig

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