scholarly journals Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase

2021 ◽  
pp. 103885
Author(s):  
Katarina Novović ◽  
Milka Malešević ◽  
Lazar Gardijan ◽  
Milan Kojić ◽  
Branko Jovčić
2008 ◽  
Vol 74 (15) ◽  
pp. 4847-4852 ◽  
Author(s):  
Anastasia Matthies ◽  
Thomas Clavel ◽  
Michael Gütschow ◽  
Wolfram Engst ◽  
Dirk Haller ◽  
...  

ABSTRACT The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.


2010 ◽  
Vol 8 (1) ◽  
pp. 51 ◽  
Author(s):  
Niurka Meneses ◽  
Guillermo Mendoza-Hernández ◽  
Sergio Encarnación

PROTEOMICS ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 1800116 ◽  
Author(s):  
Micaela Cerletti ◽  
María Ines Giménez ◽  
Christian Tröetschel ◽  
Celeste D’ Alessandro ◽  
Ansgar Poetsch ◽  
...  

1979 ◽  
Vol 42 (11) ◽  
pp. 848-851 ◽  
Author(s):  
Y. PARK ◽  
E. M. MIKOLAJCIK

Growth and alpha toxin production by a strain of Clostridium perfringens was determined in Thioglycollate medium, beef broth with ground beef, and beef broth with ground beef and soy protein. Incubation temperatures ranged from 15 to 50 C. In Thioglycollate medium, maximum alpha toxin production occurred at 35 C and was 40 times greater than that observed at 45 C. However, generation time and maximum population were approximately the same at 35 and 45 C. At 15 C, a two log cycle reduction in viable counts occurred within 6 h. Irrespective of incubation temperature, alpha toxin levels in Thioglycollate medium declined as the incubation period was extended beyond the stationary growth phase. In the beef broth with ground beef system which was studied at 35 C only, the organism grew slower and produced less toxin than in Thioglycollate medium. The amount of alpha toxin detected was influenced to a greater extent by the incubation time and temperature, the holding time beyond the stationary growth phase, and the growth medium than by the population level of C. perfringens.


RNA Biology ◽  
2016 ◽  
Vol 13 (4) ◽  
pp. 427-440 ◽  
Author(s):  
Efthimia Lioliou ◽  
Pierre Fechter ◽  
Isabelle Caldelari ◽  
Brian C. Jester ◽  
Sarah Dubrac ◽  
...  

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 198 ◽  
Author(s):  
Manoj K. Singh ◽  
Pradeep K. Rai ◽  
Anuradha Rai ◽  
Surendra Singh ◽  
Jay Shankar Singh

The production of poly-β-hydroxybutyrate (PHB) under varying environmental conditions (pH, temperature and carbon sources) was examined in the cyanobacterium Scytonema geitleri Bharadwaja isolated from the roof-top of a building. The S. geitleri produced PHB and the production of PHB was linear with the growth of cyanobacterium. The maximum PHB production (7.12% of dry cell weight) was recorded when the cells of S. geitleri were at their stationary growth phase. The production of PHB was optimum at pH 8.5 and 30 °C, and acetate (30 mM) was the preferred carbon source.


2007 ◽  
Vol 104 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Takeko Kodama ◽  
Keiji Endo ◽  
Kazuhisa Sawada ◽  
Katsutoshi Ara ◽  
Katsuya Ozaki ◽  
...  

2017 ◽  
Vol 10 (6) ◽  
Author(s):  
Hadi Koohsari ◽  
Ezzat Allah Ghaemi ◽  
Noor Amir Mozaffari ◽  
Abdolvahhab Moradi ◽  
Maryam Sadegh-Sheshpoli ◽  
...  

1998 ◽  
Vol 61 (12) ◽  
pp. 1657-1660 ◽  
Author(s):  
E. PALOU ◽  
A. LÓPEZ-MALO ◽  
G. V. BARBOSA-CÁNOVAS ◽  
J. WELTI-CHANES ◽  
P. M. DAVIDSON ◽  
...  

The effects of the come-up time at selected pressures (50 to 689 MPa) on Saccharomyces cerevisiae and Zygosaccharomyces bailii viability were evaluated at 21°C. For Z. bailii the effects of the water activity (aw) of the suspension media and the stage of the growth cycle were also investigated. Pressure come-up times exerted an important effect on the yeast survival fraction, decreasing counts as pressure increased. An increased sensitivity to pressure treatments was observed with yeast cells from the exponential growth phase. Lethality increased as aw of the suspension media increased. For an aw of 0.98 and cells from the stationary growth phase, pressure treatments at less than 200 MPa had no effect on Z. bailii viability; however, no survivors (<10 CFU/ml) were observed in treatments applied only for the time needed to reach pressures greater than 517 MPa. Yeast survivor curves showed an excellent fit (r > 0.996) when described by a phenomenological model based on the Fermi equation, S(P) = 1/|1 + exp[(P − Pc)/k]|, where S(P) is the survival fraction, P is the pressure, Pc is a critical pressure corresponding to 50% survival, and k is a constant representing the steepness of the curve.


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