Mast cells and Kurloff cells - Their detection throughout the oestrous cycle in normal guinea pig ovaries and in guinea pigs with cystic rete ovarii

2021 ◽  
Vol 136 ◽  
pp. 512-518
Author(s):  
Pavla Hamouzova ◽  
Petr Cizek ◽  
Vladimir Jekl ◽  
Karolina Gozdziewska-Harlajczuk ◽  
Joanna Kleckowska-Nawrot
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Oestrous Cycle

PRODUCTION OF PROSTAGLANDINS BY THE GUINEA-PIG UTERUS

1972 ◽  
Vol 54 (1) ◽  
pp. 147-159 ◽  
Author(s):  
N. L. POYSER
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Oestrous Cycle ◽  
Prostaglandin E ◽  
Prostaglandin F

SUMMARY The production of prostaglandins by the uterus and the resting levels of prostaglandins in the uterus on selected days of the oestrous cycle were determined in guinea-pigs. Prostaglandin F2α was detectable in the guinea-pig uterus in small amounts on days 13, 14 and 15 of the cycle. Prostaglandin E2 was present in even smaller amounts on days 14 and 15. The homogenized guinea-pig uterus had the ability to biosynthesize prostaglandins, from endogenous precursors, during incubation on every day of the cycle studied. Four to six times more prostaglandin F2α than E2 was produced on any one day with the amounts of prostaglandins formed increasing towards the end of the oestrous cycle. Indomethacin inhibited the biosynthesis of prostaglandins by the guinea-pig uterus. The implications of these findings are discussed.

HYSTERECTOMY OF THE NEWBORN GUINEA-PIG, SUBSEQUENT EFFECTS ON THE OESTROUS CYCLE AND LIFE SPAN OF THE CORPORA LUTEA

1975 ◽  
Vol 66 (2) ◽  
pp. 233-237 ◽  
Author(s):  
W. BUÑO ◽  
E. CARLEVARO ◽  
L. RIBONI ◽  
H. D'ALBORA ◽  
L. DE LOS REYES ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Life Span ◽  
Guinea Pigs ◽  
Adult Life ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Long Cycles

SUMMARY Longer oestrous cycles result from neonatal hysterectomy than from hysterectomy in adult life. Section and cauterization of the utero-vaginal union also prolonged the vaginal closure period up to an average of 55 days. The destruction of the mesometrium did not lengthen the oestrous cycle. Uterine autografts in hysterectomized newborn guinea-pigs did not prevent the long cycles.

THE EFFECT OF AUTOTRANSPLANTATION OF THE OVARIES TO THE KIDNEYS OR UTERUS ON THE OESTROUS CYCLE OF THE GUINEA-PIG

1968 ◽  
Vol 41 (1) ◽  
pp. 95-103 ◽  
Author(s):  
K. P. BLAND ◽  
B. T. DONOVAN
Keyword(s):  
Guinea Pig ◽  
Functional State ◽  
Guinea Pigs ◽  
Follicular Development ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Primordial Follicles ◽  
Local Nature

SUMMARY Autotransplantation of the ovaries of guinea-pigs to either the uterus or the kidneys caused the degeneration of all luteal and follicular tissue with the exception of the primordial follicles situated in the periphery of the graft. Follicular development then took place and oestrus and ovulation occurred 10–11 days after transplantation. The corpora lutea formed at this ovulation were maintained in a functional state for more than 35 days when the ovaries were transferred to the kidneys but when ovarian grafts were made to the uterus a series of shortened vaginal cycles was observed. These results substantiate the local nature of the luteolytic abilities of the uterus in this species and imply the existence of a uterine luteolytic substance.

THE EFFECT OF HYPOPHYSIAL STALK-SECTION ON THE CORPUS LUTEUM OF THE GUINEA-PIG

1971 ◽  
Vol 50 (4) ◽  
pp. 625-635 ◽  
Author(s):  
DOREEN V. ILLINGWORTH ◽  
J. S. PERRY
Keyword(s):  
Growth Rate ◽  
Guinea Pig ◽  
Corpus Luteum ◽  
Guinea Pigs ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Normal Cycle ◽  
Luteal Regression ◽  
Normal Regression ◽  
And Function

SUMMARY The effects of hypophysial stalk-section on the growth and function of the corpus luteum of the non-pregnant guinea-pig have been compared with the effects of hypophysectomy (as previously described) and with the effects of prolactin administered to hypophysectomized animals. Stalk-section soon after ovulation did not impair the growth of the corpora lutea nor their ability to secrete progesterone. Stalk-section before day 9 of the oestrous cycle prevented the normal regression of the corpora lutea; they continued to grow and 3 weeks after ovulation were as large as those of pregnant animals, or of non-pregnant hysterectomized guinea-pigs. The corpora lutea regressed irregularly during the following 2 weeks. When performed on, or later than day 9, stalk-section did not prevent luteal regression at the normal time. Administration of prolactin (10 i.u./day) to hypophysectomized guinea-pigs restored the growth-rate of the corpora lutea, which reached sizes comparable to those of the normal cycle, and those of stalk-sectioned animals, by 10 days after ovulation. Our results indicate that prolactin can have substantial luteotrophic activity in the guinea-pig.

OESTROGEN AND PROGESTERONE AND THE FUNCTION OF THE CORPORA LUTEA IN THE GUINEA-PIG

1970 ◽  
Vol 47 (2) ◽  
pp. 225-230 ◽  
Author(s):  
K. P. BLAND ◽  
B. T. DONOVAN
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Single Injection ◽  
Glass Beads ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Luteal Regression ◽  
Oestradiol Benzoate

SUMMARY Regression of the corpora lutea in the guinea-pig could be accelerated by treatment with 10 μg. oestradiol benzoate daily over days 3–11 of the oestrous cycle. A single injection of 10 μg. oestradiol benzoate on day 3 was also effective. The luteolytic effect of oestrogen was abolished by hysterectomy, indicating that the uterus is involved in the mediation of the response. Progesterone (5 mg. daily over days 3–11) enhanced the luteal regression brought about by the presence of two glass beads in one horn of the uterus, but did not affect luteal size in otherwise intact females. Treatment of guinea-pigs with 5 mg. progesterone daily over days 2–5 or 4–7 failed to alter the length of the oestrous cycle.

scholarly journals DISTRIBUTION AND QUANTITATIVE CHANGES OF MAST CELLS IN GUINEA PIGS LUNG IN OVALBUMIN-INDUCED ALLERGIC INFLAMMATION

2021 ◽  
Vol 7 (1) ◽  
pp. 87-92
Author(s):  
S. S. Popko ◽  
V. M. Yevtushenko
Keyword(s):  
Mast Cells ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Early Period ◽  
Allergic Inflammation ◽  
Cell Types ◽  
Maximum Increase ◽  
Local Immunity ◽  
Quantitative Changes ◽  
Experimental Group

Background. One of the most important cells in local immunity in lung are mast cells. They are involved in both innate and adaptive immune responses to inhaled allergens. The question of distribution of these both cell types in guinea pig lung in case of experimental allergic inflammation in most aspects remains open. Objective. The aim of this research is to study the distribution and quantitative changes of mast cells in lung of guinea pigs in ovalbumin-induced allergic inflammation. Methods. the lungs of 48 male guinea pigs have been studied using histological, morphometric and statistical methods in cases of experimental ovalbumin-induced allergic inflammation. The total number of mucosa related must cells and perivascular mast cells in guinea pig lungs were counted. Results. It has been established that mucosa related mast cells are normally more abundant in guinea pigs lung than perivascular ones. Maximum increase in a number of mucosa related mast cells was revealed in the early period of allergic inflammation, as evidenced by maximum increase coefficient of 1.4 in the 1st experimental group, compare to the control (P*/**<0.05). However, maximum increase in number of perivascular mast cells in 5 times was found during the late period of allergic inflammation in the 4th experimental group (P*/**<0.05). Conclusion. Experimental sensitization and challenge with ovalbumin leads to statistically significant increase in average number of both types of mast cells but predominantly the latter ones. It has been proved that cells dynamics is multidirectional.

SOME OBSERVATIONS ON THE EXCRETION OF 17-KETOSTEROIDS AND 17-KETOGENIC STEROIDS BY GUINEA-PIGS

1960 ◽  
Vol 20 (1) ◽  
pp. 24-35 ◽  
Author(s):  
R. V. BROOKS ◽  
BARBARA E. CLAYTON ◽  
JOYCE E. HAMMANT
Keyword(s):  
Guinea Pig ◽  
Urinary Excretion ◽  
Guinea Pigs ◽  
Oestrous Cycle ◽  
Pregnant Mother ◽  
Cyclical Change ◽  
Urinary Steroids ◽  
Adrenalectomized Animal ◽  
Consistent Trend

SUMMARY 1. 17-Ketosteroids (KS) and 17-ketogenic steroids (KG) may be determined in guinea-pig urine by the method of Norymberski, Stubbs & West [1953]. Studies on the fractionation of urinary steroids showed the method was applicable to the urine of pregnant guinea-pigs. 2. So little material in the faeces of guinea-pigs behaved as KS that excretion by this route may be ignored. 3. Daily vaginal lavages shortened the oestrous cycle in the guinea-pig and abolished the peak of KS excretion seen at oestrus in normal cycles. No consistent trend in the excretion of KG was seen during normal oestrous cycles. No cyclical change in KS excretion was seen after ovariectomy. 4. During pregnancy in the guinea-pig, there was a rise in the excretion of KG and KS. The increase was not abolished by ovariectomy. The rise did not occur in one adrenalectomized animal which went to term, though it continued to excrete significant amounts of KS. The contribution of the foetal adrenals to the excretion of steroids by the pregnant mother was probably small. 5. Non-specific adrenal stimulation caused a rise in the urinary excretion of KS and KG. 6. Fractionation of the KS released from the urine of an intact non-pregnant guinea-pig by a mild hydrolytic procedure gave five major compounds, one in the 11-deoxy and four in the 11-oxy fraction. All five compounds were excreted in increased amounts by a pregnant intact and by a pregnant adrenalectomized guinea-pig.

ALTERATION IN THE OESTROUS CYCLE LENGTH AFTER PITUITARY HOMOTRANSPLANTATION IN THE GUINEA-PIG

1970 ◽  
Vol 48 (2) ◽  
pp. 277-283
Author(s):  
R. M. DAS ◽  
G. K. BENSON
Keyword(s):  
Guinea Pig ◽  
Adult Female ◽  
Guinea Pigs ◽  
Cycle Length ◽  
Oestrous Cycle ◽  
Kidney Capsule ◽  
Graft Tissue

SUMMARY Pituitaries from adult or young guinea-pigs were implanted under the kidney capsule of intact adult female guinea-pigs between the 3rd and 5th days of the oestrous cycle. The oestrous cycles in the recipients of adult pituitaries were mostly normal in length. Seven out of nine animals engrafted with pituitaries from their own litters had a prolonged dioestrus, ranging from 8 to 16 days longer than normal, at the end of which oestrus and ovulation occurred. When the grafts contained pituitaries from one male and one female pup the cycle extension was more pronounced than when they were composed of pituitaries from two or three female pups. The occurrence of prolonged dioestrus accompanied by a delayed ovulation in the graft-bearing animals is suggested to be associated with the production of luteotrophic hormone by the graft tissue, possibly deriving from basophil cells.

PRODUCTION OF PROSTAGLANDINS BY THE EARLY PREGNANT GUINEA-PIG UTERUS IN VITRO

1974 ◽  
Vol 61 (2) ◽  
pp. 265-271 ◽  
Author(s):  
FELICITY M. MAULE WALKER ◽  
N. L. POYSER
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Significant Difference

SUMMARY The amounts of prostaglandins F2α (PGF2α) and E2 (PGE2) produced by homogenized, incubated, pregnant and non-pregnant guinea-pig uteri were compared. The sizes of the corpora lutea from these animals were considered in relation to the amounts of prostaglandins produced by their uteri. The amount of PGF2α produced by the uteri from day-15 bilaterally pregnant guinea-pigs was significantly less than that produced by uteri from guinea-pigs on day 15 of the oestrous cycle. Corpora lutea from the pregnant animals were maintained but those from the day 15 non-pregnant animals had regressed. There was no significant difference between the results obtained for PGE2. Results from day 15 unilaterally pregnant animals and day 25 bilaterally pregnant animals are also presented and their implications discussed. The findings reported here may well explain how, in the guinea-pig, the conceptus is able to neutralize the luteolytic effects of the uterus.

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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