Physiological change and screening of differentially expressed genes of wild Chinese Vitis yeshanensis and American Vitis riparia in response to drought stress

Abstract Phosphorus stress and drought stress are common abiotic stresses. In this study, two winter wheat “Xindong20” and “Xindong23” were solution cultured and then treated with drought stress under conventional phosphorus level (CP: 1.0 mmol/L) and low phosphorus level (LP: 0.05 mmol /L), respectively. The results showed that with the increase of drought stress, the LP application was more conducive to the growth of root tips, length, forks, surfarea and root vitality of wheat. Under the LP treatment, the total phosphorus content of root at rewatered 3d was increased by 94.2% in Xindong20 wheat and decreased by 48.9% in Xindong23 wheat, compared with their respective samples at drought 0d. The LP treatment increased the percentage content of K and decreased the P and Ca percentage content. However, under CP treatment, the percentage content of Zn after rewatered 3 days were increased, compared with drought 7d. Based on the GeneChip analysis of root samples from drought 7d, the microarray results showed that 4577 and 202 differentially expressed genes were detected from Xindong20 and Xindong23, respectively. Among them, 89.9% of differentially expressed genes were involved in organelles and vesicles in Xindong20, and 69.8% were involved in genes encoding root anatomical structure, respiratory chain, electron transport chain, ion transport and enzyme activity in Xindong23. Therefore, the supply of low phosphorus has more effects on the drought tolerance of wheat, and the wheat with different drought tolerance has different regulatory genes. The higher drought-tolerant wheat has more genes up-regulation in response to drought stress.

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Inferring the genetic responses to acute drought stress across an ecological gradient

BMC Genomics ◽

10.1186/s12864-021-08178-w ◽

2022 ◽

Vol 23 (1) ◽

Author(s):

Jessica K. Devitt ◽

Albert Chung ◽

John J. Schenk

Keyword(s):

Drought Stress ◽

Differentially Expressed Genes ◽

Target Genes ◽

De Novo ◽

Water Movement ◽

Model Systems ◽

Differentially Expressed ◽

Model Organisms ◽

Ecological Gradient ◽

Genetic Responses

Abstract Background How do xerophytic species thrive in environments that experience extreme annual drought? Although critical to the survival of many species, the genetic responses to drought stress in many non-model organisms has yet to be explored. We investigated this question in Mentzelia section Bartonia (Loasaceae), which occurs throughout western North America, including arid lands. To better understand the genetic responses to drought stress among species that occur in different habitats, the gene expression levels of three species from Mentzelia were compared across a precipitation gradient. Two de novo reference transcriptomes were generated and annotated. Leaf and root tissues were collected from control and drought shocked plants and compared to one another for differential expression. A target-gene approach was also implemented to better understand how drought-related genes from model and crop species function in non-model systems. Results When comparing the drought-shock treatment plants to their respective control plants, we identified 165 differentially expressed clusters across all three species. Differentially expressed genes including those associated with water movement, photosynthesis, and delayed senescence. The transcriptome profiling approach was coupled with a target genes approach that measured expression of 90 genes associated with drought tolerance in model organisms. Comparing differentially expressed genes with a ≥ 2 log-fold value between species and tissue types showed significant differences in drought response. In pairwise comparisons, species that occurred in drier environments differentially expressed greater genes in leaves when drought shocked than those from wetter environments, but expression in the roots mostly produced opposite results. Conclusions Arid-adapted species mount greater genetic responses compared to the mesophytic species, which has likely evolved in response to consistent annual drought exposure across generations. Drought responses also depended on organ type. Xerophytes, for example, mounted a larger response in leaves to downregulate photosynthesis and senescence, while mobilizing carbon and regulating water in the roots. The complexity of drought responses in Mentzelia suggest that whole organism responses need to be considered when studying drought and, in particular, the physiological mechanisms in which plants regulate water, carbon, cell death, metabolism, and secondary metabolites.

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Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage

Journal of Experimental Botany ◽

10.1093/jxb/erp194 ◽

2009 ◽

Vol 60 (12) ◽

pp. 3531-3544 ◽

Cited By ~ 233

Author(s):

Peiguo Guo ◽

Michael Baum ◽

Stefania Grando ◽

Salvatore Ceccarelli ◽

Guihua Bai ◽

...

Keyword(s):

Drought Stress ◽

Differentially Expressed Genes ◽

Reproductive Stage ◽

Differentially Expressed ◽

Drought Tolerant ◽

Barley Genotypes

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Identification of differentially expressed genes in Alternanthera philoxeroides under drought stress using suppression subtractive hybridization

Russian Journal of Plant Physiology ◽

10.1134/s1021443715010094 ◽

2015 ◽

Vol 62 (1) ◽

pp. 93-100 ◽

Cited By ~ 2

Author(s):

D. Jia ◽

B. Zhang ◽

P. P. Zhang ◽

J. Y. Zhang ◽

Y. H. Liu ◽

...

Keyword(s):

Drought Stress ◽

Differentially Expressed Genes ◽

Suppression Subtractive Hybridization ◽

Subtractive Hybridization ◽

Differentially Expressed ◽

Alternanthera Philoxeroides

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The Landscape of the Genomic Distribution and the Expression of the F-Box Genes Unveil Genome Plasticity in Hexaploid Wheat during Grain Development and in Response to Heat and Drought Stress

International Journal of Molecular Sciences ◽

10.3390/ijms22063111 ◽

2021 ◽

Vol 22 (6) ◽

pp. 3111

Author(s):

Claire Guérin ◽

Saïd Mouzeyar ◽

Jane Roche

Keyword(s):

Drought Stress ◽

Differentially Expressed Genes ◽

Gene Families ◽

Wheat Genome ◽

26S Proteasome ◽

Differentially Expressed ◽

Small Scale ◽

Plant Responses ◽

Genomic Distribution ◽

Complex Events

FBX proteins are subunits of the SCF complex (Skp1–cullin–FBX) belonging to the E3 ligase family, which is involved in the ubiquitin–proteasome 26S (UPS) pathway responsible for the post-translational protein turnover. By targeting, in a selective manner, key regulatory proteins for ubiquitination and 26S proteasome degradation, FBX proteins play a major role in plant responses to diverse developmental and stress conditions. Although studies on the genomic organization of the FBX gene family in various species have been reported, knowledge related to bread wheat (Triticum aestivum) is scarce and needs to be broadened. Using the latest assembly of the wheat genome, we identified 3670 TaFBX genes distributed non-homogeneously within the three subgenomes (A, B and D) and between the 21 chromosomes, establishing it as one of the richest gene families among plant species. Based on the presence of the five different chromosomal regions previously identified, the present study focused on the genomic distribution of the TaFBX family and the identification of differentially expressed genes during the embryogenesis stages and in response to heat and drought stress. Most of the time, when comparing the expected number of genes (taking into account the formal gene distribution on the entire wheat genome), the TaFBX family harbors a different pattern at the various stratum of observation (subgenome, chromosome, chromosomal regions). We report here that the local gene expansion of the TaFBX family must be the consequence of multiple and complex events, including tandem and small-scale duplications. Regarding the differentially expressed TaFBX genes, while the majority of the genes are localized in the distal chromosomal regions (R1 and R3), differentially expressed genes are more present in the interstitial regions (R2a and R2b) than expected, which could be an indication of the preservation of major genes in those specific chromosomal regions.

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