scholarly journals Aerobic composting reduces antibiotic resistance genes in cattle manure and the resistome dissemination in agricultural soils

2018 ◽  
Vol 612 ◽  
pp. 1300-1310 ◽  
Author(s):  
Min Gou ◽  
Hang-Wei Hu ◽  
Yu-Jing Zhang ◽  
Jun-Tao Wang ◽  
Helen Hayden ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soils ◽  
Antibiotic Resistance Genes ◽  
Cattle Manure ◽  
Aerobic Composting

scholarly journals Functional metagenomic characterization of antibiotic resistance genes in agricultural soils from China

2014 ◽  
Vol 65 ◽  
pp. 9-15 ◽  
Author(s):  
Jian Qiang Su ◽  
Bei Wei ◽  
Chun Yan Xu ◽  
Min Qiao ◽  
Yong Guan Zhu
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soils ◽  
Antibiotic Resistance Genes

scholarly journals Resilience of agricultural soils to antibiotic resistance genes introduced by agricultural management practices

2021 ◽  
Vol 756 ◽  
pp. 143699
Author(s):  
Elena Radu ◽  
Markus Woegerbauer ◽  
Gerhard Rab ◽  
Matthias Oismüller ◽  
Peter Strauss ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Management Practices ◽  
Agricultural Soils ◽  
Antibiotic Resistance Genes ◽  
Agricultural Management

scholarly journals Impact of Long-Term Manure and Sewage Sludge Application to Soil as Organic Fertilizer on the Incidence of Pathogenic Microorganisms and Antibiotic Resistance Genes

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1423
Author(s):  
Hana Stiborova ◽  
Martina Kracmarova ◽  
Tereza Vesela ◽  
Marta Biesiekierska ◽  
Jindrich Cerny ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Sewage Sludge ◽  
Relative Abundance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Agricultural Soils ◽  
Organic Fertilizer ◽  
Antibiotic Resistance Genes ◽  
Thermophilic Conditions ◽  
Repeated Applications

The reuse of stabilized (under thermophilic conditions) sewage sludge and manure on agricultural soils is a common practice. The aim of this study was to evaluate the risks associated with their repeated applications on the spread of pathogenic bacteria and antibiotic resistance genes (ARGs) that encode resistance to tetracycline (tetA and tetW), sulphonamide (sul1 and sul2), erythromycin (ermB), vancomycin (vanA) and integron genetic element (intI1). The trial fields has been regularly fertilized every 3rd year since 1996 with manure (MF; 330 kg N/ha) and sewage sludge (SF; 330 kg N/ha and SF3; 990 kg N/ha). Unfertilized soil (CF) served as a control. Samples were collected at different time points: (i) right before fertilization (which was also 3 years after the last fertilization), (ii) 5 months after fertilization, and (iii) 11 months after fertilization. The relative abundance of amplicon sequence variants (ASVs) assigned to potentially pathogenic bacteria was low (0.3% and 0.25% in sludge and manure, respectively), and no association with the application of these fertilizers was found. On the other hand, our data indicate that an increased relative abundance of the ARGs sul1 and tetW was significantly associated with these fertilizer applications, and sul1 was increased in all treatments regardless of the time. It is suggested that sul1 should be monitored in organically fertilized soils to prevent its spread and possible further accumulation in crops.

scholarly journals Herbicide selection promotes antibiotic resistance in soil microbiomes

2021 ◽  
Author(s):  
Hanpeng Liao ◽  
Xi Li ◽  
Qiue Yang ◽  
Yudan Bai ◽  
Peng Cui ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soils ◽  
Soil Microbial Communities ◽  
Antibiotic Resistance Genes ◽  
Cell Membrane Permeability ◽  
Herbicide Application ◽  
Soil Microbial ◽  
Resistance Plasmids ◽  
Selection For

Abstract Herbicides are one of the most widely used chemicals in agriculture. While they are known to be harmful to non-target organisms, the effects of herbicides on the composition and functioning of soil microbial communities remain unclear. Here we show that application of three widely used herbicides—glyphosate, glufosinate and dicamba—increase the prevalence of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in soil microbiomes without clear changes in the abundance, diversity and composition of bacterial communities. Mechanistically, these results could be explained by a positive selection for more tolerant genotypes that acquired several mutations in previously well-characterized herbicide and antibiotic resistance genes. Moreover, herbicide exposure increased cell membrane permeability and conjugation frequency of multidrug resistance plasmids, promoting ARG movement between bacteria. A similar pattern was found in agricultural soils across eleven provinces in China, where herbicide application, and the levels of glyphosate residues in soils, were associated with increased ARG and MGE abundances relative to herbicide-free control sites. Together, our results show that herbicide application can enrich ARGs and MGEs by changing the genetic composition of soil microbiomes, potentially contributing to the global antimicrobial resistance problem in agricultural environments.

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