AbstractChironomid assemblages in thirty-three mountain lakes situated above tree line in the Slovakian part of the Tatra Mountains were studied during 2000–2002. Chironomid species/taxa, collected as pupal exuviae, were correlated with physical, chemical, and lake morphometry variables of 22 lakes. Two-way indicator species analysis (TWINSPAN) was used to classify the lakes into four distinct groups: higher situated alpine lakes, lower situated alpine lakes, subalpine lakes and acidified lakes. Presence/absence of eight taxa was identified as indicative for this classification. In discriminant function analysis, pH, dissolved organic carbon, altitude and lake area were the most significant variables reflecting differences among groups of lakes. This model of four variables allowed 77% success in the prediction of group membership. A multiple regression model with lake area, concentration of magnesium and total phosphorus accounted for 37% of the variance in taxa richness. Lakes with greater area contained more chironomid taxa than smaller ones. Lakes with higher alkalinity and higher trophic status tend to support more taxa. Canonical correspondence analysis (CCA) indicated that most variation in the composition of chironomid assemblages was related to pH and to altitude. The results can be used as reference data for long-term monitoring of the Tatra lakes, especially in connection with a recovery from acidification and global climatic change.