Efficient municipal wastewater treatment by oxidation ditch process at low temperature: Bacterial community structure in activated sludge

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9-21.6 degrees C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD x g TSS(-1) x d(-1) were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9 degrees C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0:02) and organic load rate (R = -0.55, p = 0.04).

Download Full-text

Analysis of Bacterial Community Structure of Activated Sludge from Wastewater Treatment Plants in Winter

BioMed Research International ◽

10.1155/2018/8278970 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 15

Author(s):

Shuang Xu ◽

Junqin Yao ◽

Meihaguli Ainiwaer ◽

Ying Hong ◽

Yanjiang Zhang

Keyword(s):

Wastewater Treatment ◽

Community Structure ◽

Bacterial Community ◽

Activated Sludge ◽

Bacterial Community Structure ◽

Redundancy Analysis ◽

Wastewater Treatment Plants ◽

Effluent Treatment ◽

Autonomous Region ◽

Sludge Bulking

Activated sludge bulking is easily caused in winter, resulting in adverse effects on effluent treatment and management of wastewater treatment plants. In this study, activated sludge samples were collected from different wastewater treatment plants in the northern Xinjiang Uygur Autonomous Region of China in winter. The bacterial community compositions and diversities of activated sludge were analyzed to identify the bacteria that cause bulking of activated sludge. The sequencing generated 30087–55170 effective reads representing 36 phyla, 293 families, and 579 genera in all samples. The dominant phyla present in all activated sludge were Proteobacteria (26.7–48.9%), Bacteroidetes (19.3–37.3%), Chloroflexi (2.9–17.1%), and Acidobacteria (1.5–13.8%). Fifty-five genera including unclassified_f_Comamonadaceae, norank_f_Saprospiraceae, Flavobacterium, norank_f_Hydrogenophilaceae, Dokdonella, Terrimonas, norank_f_Anaerolineaceae, Tetrasphaera, Simplicispira, norank_c_Ardenticatenia, and Nitrospira existed in all samples, accounting for 60.6–82.7% of total effective sequences in each sample. The relative abundances of Saprospiraceae, Flavobacterium, and Tetrasphaera with the respective averages of 12.0%, 8.3%, and 5.2% in bulking sludge samples were higher than those in normal samples. Filamentous Saprospiraceae, Flavobacterium, and Tetrasphaera multiplied were the main cause for the sludge bulking. Redundancy analysis (RDA) indicated that influent BOD5, DO, water temperature, and influent ammonia had a distinct effect on bacterial community structures.

Download Full-text

Comparison of Nitrate and Nitrite Denitrification Rates of Activated Sludge Samples Collected from Municipal Wastewater Treatment Plants and Analysis of its Denitrifier Community Structure

Journal of Japan Society on Water Environment ◽

10.2965/jswe.30.585 ◽

2007 ◽

Vol 30 (10) ◽

pp. 585-588

Author(s):

Tadashi NITTAMI ◽

Hiroaki OOTAKE ◽

Kazuho NAKAMURA ◽

Kanji MATSUMOTO

Keyword(s):

Wastewater Treatment ◽

Community Structure ◽

Activated Sludge ◽

Municipal Wastewater ◽

Wastewater Treatment Plants ◽

Municipal Wastewater Treatment ◽

Municipal Wastewater Treatment Plants ◽

Nitrate And Nitrite

Download Full-text

Community Composition and Function of Bacteria in Activated Sludge of Municipal Wastewater Treatment Plants

Water ◽

10.3390/w13060852 ◽

2021 ◽

Vol 13 (6) ◽

pp. 852 ◽

Cited By ~ 1

Author(s):

Ning Xie ◽

Liping Zhong ◽

Liao Ouyang ◽

Wang Xu ◽

Qinghuai Zeng ◽

...

Keyword(s):

Wastewater Treatment ◽

Community Structure ◽

Activated Sludge ◽

Municipal Wastewater ◽

Wastewater Treatment Plants ◽

16S Rrna Genes ◽

Rrna Genes ◽

Municipal Wastewater Treatment ◽

Significant Difference ◽

Municipal Wastewater Treatment Plants

Municipal wastewater treatment plants (WWTPs) use functional microorganisms in activated sludge (AS) to reduce the environmental threat posed by wastewater. In this study, Illumina NovaSeq sequencing of 16S rRNA genes was performed to explore the microbial communities of AS at different stages of the two WWTP projects in Shenzhen, China. Results showed that Proteobacteria, Bacteroidetes, Acidobacteria, Firmicutes, and Nitrospirae were the dominant phyla in all the samples, with Proteobacteria being the most abundant and reaching a maximum proportion of 59.63%. There was no significant difference in biodiversity between the two water plants, but Stage 1 and Stage 2 were significantly different. The Mantel test indicated that nitrate, total nitrogen (TN), chemical oxygen demand (COD), and nutrients were essential factors affecting the bacterial community structure. FAPROTAX analysis emphasized that the leading functional gene families include nitrification, aerobic nitrite oxidation, human pathogens, and phototrophy. This study reveals changes in the community structure of AS in different treatment units of Banxuegang WWTP, which can help engineers to optimize the wastewater treatment process.

Download Full-text