scholarly journals Impact of Operational Parameters on Bacterial Community in a Full-Scale Municipal Wastewater Treatment Plant

2012 ◽  
Vol 61 (1) ◽  
pp. 41-49 ◽  
Author(s):  
AGNIESZKA CYDZIK-KWIATKOWSKA ◽  
MAGDALENA ZIELIŃSKA ◽  
IRENA WOJNOWSKA-BARYŁA

A bacterial community in activated sludge from a full-scale municipal wastewater treatment plant was monitored throughout the year with the use of FISH, RISA and DGGE techniques. In the investigated range of temperatures (11.9-21.6 degrees C), a rise in temperature resulted in a lower total bacteria richness, while organic load rate changes from 0.09 to 0.21 g COD x g TSS(-1) x d(-1) were positively correlated with the number of bands in RISA patterns. The most diverse pattern (29 different bands) was characteristic for the activated sludge sample collected at the end of January at wastewater temperature of 11.9 degrees C. The ammonia-oxidising bacteria community did not change during the study, and comprised of 4 different bacterial populations with one dominant species closely related to Nitrosospira sp. REGAU (GenBank accession number AY635572.1). The percentage of ammonia-oxidising bacteria in the activated sludge varied from 6.2 to 19.5% and depended on temperature (R = 0.61, p = 0:02) and organic load rate (R = -0.55, p = 0.04).

2019 ◽  
Vol 11 (3) ◽  
pp. 622 ◽  
Author(s):  
Isabella Pecorini ◽  
Francesco Baldi ◽  
Renato Iannelli

Four inocula collected from different operating facilities were tested in their hydrogenic performances by means of two biochemical hydrogen potential test set-ups using sucrose and food waste as substrates, with the aim of evaluating the influence of inoculum media in batch fermentative assays. The selected inocula were: activated sludge collected from the aerobic unit of a municipal wastewater treatment plant, digested sludge from an anaerobic reactor treating organic waste and cattle manure, digested sludge from an anaerobic reactor treating agroindustrial residues, and digested sludge from an anaerobic reactor of a municipal wastewater treatment plant. Test results, in terms of specific hydrogen production, hydrogen conversion efficiency, and volatile solids removal efficiency, were significantly dependent on the type of inoculum. Statistical analysis showed different results, indicating that findings were due to the different inocula used in the tests. In particular, assays performed with activated sludge showed the highest performances for both substrates and both experimental set-ups.


Membranes ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 421
Author(s):  
Dimitra C. Banti ◽  
Michail Tsangas ◽  
Petros Samaras ◽  
Antonis Zorpas

Membrane bioreactor (MBR) systems are connected to several advantages compared to the conventional activated sludge (CAS) units. This work aims to the examination of the life cycle environmental impact of an MBR against a CAS unit when treating municipal wastewater with similar influent loading (BOD = 400 mg/L) and giving similar high-quality effluent (BOD < 5 mg/L). The MBR unit contained a denitrification, an aeration and a membrane tank, whereas the CAS unit included an equalization, a denitrification, a nitrification, a sedimentation, a mixing, a flocculation tank and a drum filter. Several impact categories factors were calculated by implementing the Life Cycle Assessment (LCA) methodology, including acidification potential, eutrophication potential, global warming potential (GWP), ozone depletion potential and photochemical ozone creation potential of the plants throughout their life cycle. Real data from two wastewater treatment plants were used. The research focused on two parameters which constitute the main differences between the two treatment plants: The excess sludge removal life cycle contribution—where GWPMBR = 0.50 kg CO2-eq*FU−1 and GWPCAS = 2.67 kg CO2-eq*FU−1 without sludge removal—and the wastewater treatment plant life cycle contribution—where GWPMBR = 0.002 kg CO2-eq*FU−1 and GWPCAS = 0.14 kg CO2-eq*FU−1 without land area contribution. Finally, in all the examined cases the environmental superiority of the MBR process was found.


2013 ◽  
Vol 20 (1) ◽  
pp. 199-208 ◽  
Author(s):  
Marketa Julinova ◽  
Jan Kupec ◽  
Roman Slavik ◽  
Maria Vaskova

Abstract A synthetic polymer, polyvinylpyrrolidone (PVP - E 1201) primarily finds applications in the pharmaceutical and food industries due to its resistance and zero toxicity to organisms. After ingestion, the substance passes through the organism unchanged. Consequently, it enters the systems of municipal wastewater treatment plants (WWTP) without decomposing biologically during the waste treatment process, nor does it attach (through sorption) to particles of activated sludge to any significant extent, therefore, it passes through the system of a WWTP, which may cause the substance to accumulate in the natural environment. For this reason the paper investigates the potential to initiate aerobic biodegradation of PVP in the presence of activated sludge from a municipal wastewater treatment plant. The following agents were selected as the initiators of the biodegradation process - co-substrates: acrylamide, N-acethylphenylalanine and 1-methyl-2-pyrrolidone, a substance with a similar structure to PVP monomer. The biodegradability of PVP in the presence of co-substrates was evaluated on the basis of biological oxygen demand (BOD) as determined via a MicroOxymax O2/CO2/CH4 respirometer. The total substrate concentration in the suspension equaled 400 mg·dm-3, with the ratio between PVP and the cosubstrate being 1:1, while the concentration of the dry activated sludge was 500 mg·dm-3. Even though there was no occurrence of a significant increase in the biodegradation of PVP alone in the presence of a co-substrate, acrylamide appeared to be the most effective type of co-substrate. Nevertheless, a recorded decrease in the slope of biodegradation curves over time may indicate that a process of primary decomposition was underway, which involves the production of metabolites that inhibit activated sludge microorganisms. The resulting products are not identified at this stage of experimentation.


2009 ◽  
Vol 59 (7) ◽  
pp. 1291-1297
Author(s):  
H. Poutiainen ◽  
S. Laitinen ◽  
P. Juntunen ◽  
H. Heinonen-Tanski

We describe a novel application for a microwave on-line sensor to measure the total solids (TS) load entering a municipal wastewater treatment plant (WWTP) from slaughterhouse sewage and some sanitary wastewaters. Measuring this kind of wastewater stream is very challenging, because it contains a high, but varying organic load with nitrogen, phosphorus and microorganisms. The reliability of the measured signal was studied by comparison with laboratory analyses and a correlation is presented of TS-value with other parameters that are typically followed in a wastewater treatment process. The results suggest that on-line microwave sensoring could be used to monitor total solids in wastewater influent. Our results show that the on-line microwave sensor and laboratory reference analyses give similar results with a good correlation between the two techniques. Furthermore, we demonstrate that the total solids values correlate well with conductivity, total nitrogen and BOD7 values but not with phosphorus, pH and temperature.


2014 ◽  
Vol 878 ◽  
pp. 702-707 ◽  
Author(s):  
Chang Liu ◽  
Ping Zeng ◽  
Yong Hui Song ◽  
Jian Guang Cheng ◽  
Chao Wei Zhu ◽  
...  

Three kinds of surplus activated sludge were pretreated by alkali and combination of alkali and ultrasonic. The changes of PO43-P, TP, SCOD, TOC, TS, VS before and after pretreatment were investigated. The results showed that phosphorus and organic matter could be released into supernatant both by alkali and ultrasonic. The surplus activated sludge from municipal wastewater treatment plant was easier to be broken than that from pharmaceutical wastewater treatment plant. The activated sludge from Qinghe wastewater treatment plant could be selected for further phosphorus recovery since the largest quantity of phosphorus release and the lowest organic matter release ratio among the three kinds of surplus activated sludge.


2013 ◽  
Vol 845 ◽  
pp. 538-542 ◽  
Author(s):  
Muhammad Sani Gaya ◽  
Norhaliza Abdul Wahab ◽  
Yahya M. Sam ◽  
Sharatul Izah Samsuddin

Activated sludge process is the most efficient technique used for municipal wastewater treatment plants. However, a pH value outside the limit of 6-9 could inhibit the activities of microorganisms responsible for treating the wastewater, and low pH value may cause damage to the treatment system. Therefore, prediction of pH value is essential for smooth and trouble-free operation of the process. This paper presents an adaptive neuro-fuzzy inference system (ANFIS) model for effluent pH quality prediction in the process. For comparison, artificial neural network is used. The model validation is achieved through use of full-scale data from the domestic wastewater treatment plant in Kuala Lumpur, Malaysia. Simulation results indicate that the ANFIS model predictions were highly accurate having the root mean square error (RMSE) of 0.18250, mean absolute percentage deviation (MAPD) of 9.482% and the correlation coefficient (R) of 0.72706. The proposed model is efficient and valuable tool for the activated sludge wastewater treatment process.


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