Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice

2022 ◽  
Vol 810 ◽  
pp. 152247
Author(s):  
Guo-Xiang Zhou ◽  
Wei-Bo Liu ◽  
Li-Min Dai ◽  
Hua-Long Zhu ◽  
Yong-Wei Xiong ◽  
...  
Keyword(s):  
Mitochondrial Stress ◽  
Blood Testis Barrier

Heat Stress and Pulsed Unfocused Ultrasound: The Viability of these Physical Approaches for Drug Delivery into Testicular Seminiferous Tubules

2020 ◽  
Vol 17 (5) ◽  
pp. 438-446 ◽  
Author(s):  
Yuanyuan Li ◽  
Mohammad Ishraq Zafar ◽  
Xiaotong Wang ◽  
Xiaofang Ding ◽  
Honggang Li
Keyword(s):  
Drug Delivery ◽  
Heat Stress ◽  
Targeted Drug Delivery ◽  
Therapeutic Agents ◽  
Seminiferous Tubules ◽  
Adult Mice ◽  
Targeted Drug ◽  
To Receive ◽  
Unfocused Ultrasound ◽  
Blood Testis Barrier

Aim: To investigate the application of Scrotal Heat Stress (SHS) and Pulsed Unfocused Ultrasound (PuFUS) to explore Blood-Testis Barrier (BTB) permeability in adult mice. Background: The BTB provides a stable microenvironment and a unique immune barrier for spermatogenesis. Meanwhile, it blocks macromolecular substances access, including therapeutic agents and antibodies, thereby it decreases the therapeutic or immunocontraception effects. Objectives: To determine the viability of these physical approaches in delivering macromolecular substances into seminiferous tubules. Material & Methods: Mice were subjected to receive single SHS intervention at 39°C, 41°C, or 43°C for 30 min. Whereas, mice received the PuFUS intervention at 1.75w/cm2, 1.25w/cm2, and 2.5w/cm2 for 2 min, 5 min, and 10 min, respectively. The Biotin and macromolecular substances (IgG, IgM, and exosomes) were separately injected into the testicular interstitium at different times following SHS or PuFUS interventions, to observe their penetration through BTB into seminiferous tubules. Results: As detected by Biotin tracer, the BTB opening started from day-2 following the SHS and lasted for more than three days, whereas the BTB opening started from 1.5h following PuFUS and lasted up to 24h. Apparent penetration of IgG, IgM, and exosomes into seminiferous tubules was observed after five days of the SHS at 43°C, but none at 39°C, or any conditions tested with PuFUS. Conclusion: The current results indicate that SHS at 43°C comparatively has the potential for delivering macromolecular substances into seminiferous tubules, whereas the PuFUS could be a novel, quick, and mild approach to open the BTB. These strategies might be useful for targeted drug delivery into testicular seminiferous tubules. However, further studies are warranted to validate our findings.

Semen Cuscutae-Fructus Lycii improves spermatogenic dysfunction by repairing the blood-testis barrier in rats according to in silico and in vitro methods

2021 ◽  
pp. 114022
Author(s):  
Yutian Zhu ◽  
Lei Dong ◽  
Jingshang Wang ◽  
Qiuning Liu ◽  
Hongxuan Tong ◽  
...  
Keyword(s):  
In Silico ◽  
In Vitro Methods ◽  
Fructus Lycii ◽  
Blood Testis Barrier

scholarly journals Regulation of blood-testis barrier dynamics by TGF- 3 is a Cdc42-dependent protein trafficking event

2010 ◽  
Vol 107 (25) ◽  
pp. 11399-11404 ◽  
Author(s):  
E. W. P. Wong ◽  
D. D. Mruk ◽  
W. M. Lee ◽  
C. Y. Cheng
Keyword(s):  
Protein Trafficking ◽  
Dependent Protein ◽  
Blood Testis Barrier
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