Varied interspecies interactions between anammox and denitrifying bacteria enhanced nitrogen removal in a single-stage simultaneous anammox and denitrification system

Abstract High energy costs, organic carbon availability, and space limitation are some of the barriers faced by wastewater treatment processes. This research investigates the impact of membrane aeration mode, scouring intensity, and loading rate in a single-stage total nitrogen removal process in a membrane aerated biofilm reactor (MABR). Under ammonia loading of 2.7 g N/m2.d, continuous process aeration led to 1.7 g NH4-N/m2.d and 0.8 g TN/m2.d removal, respectively. Conversely, intermittent (5/12 min on/off) aeration resulted in 35% less ammonia removal but 34% higher total nitrogen (TN) removal. The MABR under ammonia load of 1.6 g N/m2.d showed an enhanced effluent quality with an average of 2.5 mg/L effluent ammonia concentration. This finding highlights the nitrification potential of a flow-through MABR as a standalone treatment step without any downstream process. Also, slough-off, a common issue in the biofilm process and was hypothesized to reduce the removal efficiency, showed increased ammonia removal rates by 20%. The microbial analysis indicated the dominant AOB and NOB species as Nitrosomonas spp. and Nitrospira spp, respectively. Moreover, the relative abundance of denitrifying bacteria (40.5%) were found twice in intermittently-aerated MABR compared to the continuously-aerated one (20.5%). However, NOB and denitrifying bacteria relative abundances were comparable where continuous air was supplied.

Download Full-text

Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria

Applied Microbiology and Biotechnology ◽

10.1007/s00253-005-1910-9 ◽

2005 ◽

Vol 68 (1) ◽

pp. 124-130 ◽

Cited By ~ 8

Author(s):

Y. Aoi ◽

Y. Shiramasa ◽

E. Kakimoto ◽

S. Tsuneda ◽

A. Hirata ◽

...

Keyword(s):

Nitrogen Removal ◽

Denitrifying Bacteria ◽

Single Stage ◽

Composite Matrix ◽

Removal Process ◽

Autotrophic Nitrogen Removal

Download Full-text

Specific organic and nutrient loads in stabilized sludge from municipal treatment plants

Water Science & Technology ◽

10.2166/wst.1996.0342 ◽

1996 ◽

Vol 33 (12) ◽

pp. 243-250 ◽

Cited By ~ 1

Author(s):

O. Nowak ◽

A. Franz ◽

K. Svardal ◽

V. Müller

Keyword(s):

Activated Sludge ◽

Nitrogen Removal ◽

Single Stage ◽

Nutrient Loads ◽

Activated Sludge Process ◽

Waste Sludge ◽

Sludge Stabilization ◽

Nitrogen Loads ◽

Theoretical Considerations ◽

P Removal

By means of theoretical considerations and of statistical evaluations, specific organic and nitrogen loads in separately stabilized sludge have been found to be in the range of 16 to 20g VSS/PE/d and of 1.1 to 1.5 g N/PE/d respectively. About 0.6g P/PE/d are removed from the wastewater in activated sludge plants without chemical or enhanced biological P removal. By using the single-stage activated sludge process without primary sedimentation and without separate sludge stabilization, almost complete nitrogen removal can be achieved, but specific organic and nitrogen loads in the waste sludge are up to two times higher than in separately stabilized sludge.

Download Full-text

Excess nitrogen accumulation in activated sludge in sequencing batch reactor with a single-stage oxic process

Water Science & Technology ◽

10.2166/wst.2009.003 ◽

2009 ◽

Vol 59 (3) ◽

pp. 573-582 ◽

Cited By ~ 3

Author(s):

Xiao-ming Li ◽

Dong-bo Wang ◽

Qi Yang ◽

Wei Zheng ◽

Jian-bin Cao ◽

...

Keyword(s):

Activated Sludge ◽

Nitrogen Removal ◽

Sequencing Batch Reactor ◽

Ph Value ◽

Batch Reactor ◽

Nitrogen Loss ◽

Single Stage ◽

Synthetic Wastewater ◽

Nitrogen Accumulation ◽

Excess Nitrogen

It was occasionally found that a significant nitrogen loss in solution under neutral pH value in a sequencing batch reactor with a single-stage oxic process using synthetic wastewater, and then further studies were to verify the phenomenon of nitrogen loss and to investigate the pathway of nitrogen removal. The result showed that good performance of nitrogen removal was obtained in system. 0–7.28 mg L−1 ammonia, 0.08–0.38 mg L−1 nitrite and 0.94–2.12 mg L−1 nitrate were determined in effluent, respectively, when 29.85–35.65 mg L−1 ammonia was feeding as the sole nitrogen source in influent. Furthermore, a substantial nitrogen loss in solution (95% of nitrogen influent) coupled with a little gaseous nitrogen increase in off-gas (7% of nitrogen influent) was determined during a typical aerobic phase. In addition, about 322 mg nitrogen accumulation (84% of nitrogen influent) was detected in activated sludge. Based on nitrogen mass balance calculation, the unaccounted nitrogen fraction and the ratio of nitrogen accumulation in sludge/nitrogen loss in solution were 14.6 mg (3.7% of nitrogen influent) and 0.89, respectively. The facts indicated that the essential pathway of nitrogen loss in solution in this study was excess nitrogen accumulation in activated sludge.

Download Full-text

Characterization of Sludge from Single-Stage Nitrogen Removal Using Anammox and Partial Nitritation (SNAP)

Japanese Journal of Water Treatment Biology ◽

10.2521/jswtb.42.53 ◽

2006 ◽

Vol 42 (2) ◽

pp. 53-64 ◽

Cited By ~ 13

Author(s):

PHAM KHAC LIEU ◽

HAYATO HOMAN ◽

ATSUHIRO KUROGI ◽

YASUNORI KAWAGOSHI ◽

TAKAO FUJII ◽

...

Keyword(s):

Nitrogen Removal ◽

Single Stage ◽

Partial Nitritation

Download Full-text

Screening and Influencing Factors of Halophilic Denitrifying Bacteria

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1455 ◽

2012 ◽

Vol 550-553 ◽

pp. 1455-1459

Author(s):

Jing Tang ◽

Hong Ming E ◽

Jin Xiang Fu ◽

Jin Nan Chen ◽

Ming Fan

Keyword(s):

Nitrogen Removal ◽

Morphological Characteristics ◽

Development Trend ◽

Denitrifying Bacteria ◽

High Salt ◽

Research Progress ◽

Saline Wastewater ◽

Carbon And Nitrogen ◽

Petroleum Chemical ◽

Seafood Processing

With nitrite or nitrate nitrogen as electron acceptor in the high salt conditons, halophilic denitrifying bacteria can transfer nitrite or nitrate to nitrogen, thereby purifying the high-salt wastewater. Halophilic denitrifying bacteria play an important role in the carbon and nitrogen removal of saline wastewater, such as petroleum, chemical industry, seafood processing and seafood farming. This article dissussed halophilic denitrifying bacteria screening, the main types and the corresponding morphological characteristics, then we focused on the research progress of main factors of halophilic denitrifying bacteria’s growth and nitrogen removal. Finally put forward the current problems of the research and development trend of halophilic denitrifying bacteria.

Download Full-text