Effects of biofertilizer on soil microbial diversity and antibiotic resistance genes

2022 ◽  
pp. 153170
Author(s):  
Le-Yang Yang ◽  
Shu-Yi-Dan Zhou ◽  
Chen-Shuo Lin ◽  
Xin-Rong Huang ◽  
Roy Neilson ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Soil Microbial Diversity ◽  
Soil Microbial

Rare earth oxide nanoparticles promote soil microbial antibiotic resistance by selectively enriching antibiotic resistance genes

2019 ◽  
Vol 6 (2) ◽  
pp. 456-466 ◽  
Author(s):  
Lin Qi ◽  
Yuan Ge ◽  
Tian Xia ◽  
Ji-Zheng He ◽  
Congcong Shen ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Rare Earth ◽  
Microbial Communities ◽  
Resistance Genes ◽  
Soil Microbial Communities ◽  
Antibiotic Resistance Genes ◽  
Rare Earth Oxide ◽  
Oxide Nanoparticles ◽  
Soil Microbial

This study demonstrates that rare earth oxide nanoparticles can enhance soil microbial antibiotic resistance by inducing the enrichment and spread of antibiotic resistance genes in soil microbial communities.

Abundance, diversity and mobility potential of antibiotic resistance genes in pristine Tibetan Plateau soil as revealed by soil metagenomics

2020 ◽  
Vol 96 (10) ◽  
Author(s):  
Bo Li ◽  
Zeng Chen ◽  
Fan Zhang ◽  
Yongqin Liu ◽  
Tao Yan
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Soil Samples ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Metagenomic Sequencing ◽  
Clinical Issue ◽  
Abundance And Diversity

ABSTRACT Widespread occurrence of antibiotic resistance genes (ARGs) has become an important clinical issue. Studying ARGs in pristine soil environments can help to better understand the intrinsic soil resistome. In this study, 10 soil samples were collected from a high elevation and relatively pristine Tibetan area, and metagenomic sequencing and bioinformatic analyses were conducted to investigate the microbial diversity, the abundance and diversity of ARGs and the mobility potential of ARGs as indicated by different mobile genetic elements (MGEs). A total of 48 ARG types with a relative abundance of 0.05–0.28 copies of ARG/copy of 16S rRNA genes were detected in Tibetan soil samples. The observed ARGs were mainly associated with antibiotics that included glycopeptide and rifamycin; the most abundant ARGs were vanRO and vanSO. Low abundance of MGEs and potentially plasmid-related ARGs indicated a low horizontal gene transfer risk of ARGs in the pristine soil. Pearson correlation and redundancy analyses showed that temperature and total organic carbon were the major environmental factors controlling both microbial diversity and ARG abundance and diversity.

scholarly journals SOIL MICROBIAL DIVERSITY AND ANTIBIOTIC RESISTANCE IN NATURAL AND TRANSFORMED ECOSYSTEMS

2019 ◽  
Vol 9 (3) ◽  
pp. 581-590
Author(s):  
Lyudmyla Symochko ◽  
◽  
Vitaliy Symochko ◽  
Hosam E.A.F. Bayoumi Hamuda ◽  
Olena Demyanyuk ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Soil Microbial Diversity ◽  
Soil Microbial

scholarly journals Herbicide selection promotes antibiotic resistance in soil microbiomes

2021 ◽  
Author(s):  
Hanpeng Liao ◽  
Xi Li ◽  
Qiue Yang ◽  
Yudan Bai ◽  
Peng Cui ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soils ◽  
Soil Microbial Communities ◽  
Antibiotic Resistance Genes ◽  
Cell Membrane Permeability ◽  
Herbicide Application ◽  
Soil Microbial ◽  
Resistance Plasmids ◽  
Selection For

Abstract Herbicides are one of the most widely used chemicals in agriculture. While they are known to be harmful to non-target organisms, the effects of herbicides on the composition and functioning of soil microbial communities remain unclear. Here we show that application of three widely used herbicides—glyphosate, glufosinate and dicamba—increase the prevalence of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in soil microbiomes without clear changes in the abundance, diversity and composition of bacterial communities. Mechanistically, these results could be explained by a positive selection for more tolerant genotypes that acquired several mutations in previously well-characterized herbicide and antibiotic resistance genes. Moreover, herbicide exposure increased cell membrane permeability and conjugation frequency of multidrug resistance plasmids, promoting ARG movement between bacteria. A similar pattern was found in agricultural soils across eleven provinces in China, where herbicide application, and the levels of glyphosate residues in soils, were associated with increased ARG and MGE abundances relative to herbicide-free control sites. Together, our results show that herbicide application can enrich ARGs and MGEs by changing the genetic composition of soil microbiomes, potentially contributing to the global antimicrobial resistance problem in agricultural environments.

scholarly journals Prevalence and characterization of plasmid-mediated quinolone resistance in various aquatic sources

2021 ◽  
Author(s):  
Farhan Yusuf ◽  
Royce Ing
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Global Scale ◽  
Quinolone Resistance ◽  
Nutrient Loads

Ciprofloxacin is a fully synthetic antibiotic developed in 1987 and remains among the most widely used antibiotics worldwide Excessive usage has resulted in small quinolone concentrations found in aquatic sources Environments with subinhibitory antibiotic concentrations and high microbial diversity and nutrient loads (e g wastewater) foster the development of antibiotic resistance genes qnr genes conferring quinolone resistance were first found in 1998 and have since been observed on a global scale

scholarly journals Prevalence and characterization of plasmid-mediated quinolone resistance in various aquatic sources

2021 ◽  
Author(s):  
Farhan Yusuf ◽  
Royce Ing
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Global Scale ◽  
Quinolone Resistance ◽  
Nutrient Loads

Ciprofloxacin is a fully synthetic antibiotic developed in 1987 and remains among the most widely used antibiotics worldwide Excessive usage has resulted in small quinolone concentrations found in aquatic sources Environments with subinhibitory antibiotic concentrations and high microbial diversity and nutrient loads (e g wastewater) foster the development of antibiotic resistance genes qnr genes conferring quinolone resistance were first found in 1998 and have since been observed on a global scale

scholarly journals Is sewage sludge a valuable fertilizer? A soil microbiome and resistome study under field conditions

2021 ◽  
Author(s):  
Anna Markowicz ◽  
Kinga Bondarczuk ◽  
Aleksandra Wiekiera ◽  
Sławomir Sułowicz
Keyword(s):  
Antibiotic Resistance ◽  
Sewage Sludge ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Nutrient Content ◽  
Metal Resistance ◽  
Land Application ◽  
Soil Microbiome ◽  
Soil Microbial ◽  
Cu Content

Abstract Purpose Sewage sludge land application is strongly recommended to improve soil quality and fertility despite the presence of pollutants, pathogens and antibiotic resistance genes. This study aimed to assess the fertilization value of low and recommended by law sewage sludge dose (15 t ha−1). Materials and methods In a 540-day field study, the effect of sewage sludge on the soil physicochemical and microbial parameters, emphasising antibiotic and metal resistance spread, was investigated. Results In contrast to expectations, sewage sludge did not improve the organic matter, nutrient content and microbial activity in the soil; therefore, the fertilization effect was not achieved. Moreover, an increase in the bioavailable Cd, Ni and Cu content was observed. Canonical correspondence analysis revealed that these increases mainly explain the changes in the soil microbial community. Sixteen resistance genes and four integron classes were detected in both the total DNA and on plasmids isolated from sewage sludge. Obtained plasmids confer β-lactam resistance or extreme resistance to tetracycline (> 256 μg mL−1). Two antibiotic resistance genes (blaNPS-2, tetA) were transferred into the fertilized soil and detected up to 6 months after the fertilization. Conclusion Our results provide evidence that the regulated dose of sewage sludge, even when characterized by low total metal content, may affect soil microbial microbiome and resistome. Therefore, these findings provide critical data that have public health implications, which may raise concerns about the suitability of applying sewage sludge to the soil even at the low regulated dose.

scholarly journals Loss of soil microbial diversity exacerbates spread of antibiotic resistance

2019 ◽  
Vol 1 (1-2) ◽  
pp. 3-13 ◽  
Author(s):  
Qing-Lin Chen ◽  
Xin-Li An ◽  
Bang-Xiao Zheng ◽  
Michael Gillings ◽  
Josep Peñuelas ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Microbial Diversity ◽  
Soil Microbial Diversity ◽  
Soil Microbial

scholarly journals Raw wastewater irrigation for urban agriculture in Africa increases the diversity of transferable antibiotic resistances genes in soil, including those encoding ESBLs

2019 ◽  
Author(s):  
B. P. Bougnom ◽  
S. Thiele-Bruhn ◽  
V. Ricci ◽  
C. Zongo ◽  
L.J.V Piddock
Keyword(s):  
Antibiotic Resistance ◽  
Urban Agriculture ◽  
Resistance Genes ◽  
Bacterial Resistance ◽  
Antibiotic Resistance Genes ◽  
Wastewater Irrigation ◽  
Antibiotic Residues ◽  
Soil Microbial Diversity ◽  
The Impact ◽  
Raw Wastewater

AbstractA metagenomic study was conducted to investigate the impact of raw wastewater use for irrigation in urban agriculture on the development of bacterial resistance in soil. Soil samples were collected in two African countries, from three different cities (each with irrigated and non-irrigated plots). Basic physical and chemical analysis were conducted, and the presence of selected antibiotic residues was assessed. Microbial DNA was extracted, quantified and sequenced. Microbial population structure and function, presence of horizontally transferable antibiotic resistance genes andEnterobacteriaceaeplasmids replicons were analysed using bioinformatics. The relative prevalence ofProteobacteriaandBacteroidetesand sequence reads coding for microbial adaptation and growth were higher in irrigated fields; 33 and 26 transferable ARGs were found in irrigated and non-irrigated fields sequence reads, respectively. Extended spectrum β-lactam genes identified in irrigated fields includedblaCARB-3,blaOXA-347,blaOXA-5 andblaRm3. Concentration of sulfamethoxazole, ciprofloxacin and enrofloxacin in soils influenced the selection of antibiotic resistance genes encoding resistance against amphenicol, β-lactams, and tetracyclines. TenEnterobacteriaceaeplasmid amplicon groups were identified in the fields, five were common to both, two (IncW and IncP1) and three (IncY, IncFIB and IncFIA) were found in irrigated and non-irrigated fields, respectively.In conclusion, wastewater irrigation affected both soil microbial diversity and functions. Irrigated fields have more diverse transferable antibiotic resistance genes, including ESBL genes that encode resistance to β-lactams antibiotics, except cephamycins and carbapenems. Even more, critical concentrations of antibiotic residues select for multiple and cross resistance. The findings from African cities show that wastewater irrigation in urban agriculture presents a serious public health risk for farmworkers and consumers by spread of bacterial resistance.

Sign in / Sign up

Export Citation Format

Share Document