Supercritical carbon dioxide anti-solvent process for purification of micronized propolis particulates and associated anti-cancer activity

Medicinal herb Christia vespertilionis oil has been claimed to possess anti-cancer and anti-plasmodial properties and show interest in food and pharmaceutical industries. Being an important alternative medicine plant, solubility data of Christia vespertilionis oil is demanded in order to understand the separation process and is crucial for designing purposes. In this work, extraction and determination of the oil’s solubility were carried out using a green technique of supercritical carbon dioxide at a temperature range of 40 to 60℃ and 276 to 414 bar of pressure. The results demonstrated that the highest solubility was obtained at the highest temperature and pressure of 60℃ and 414 bar, respectively. For solubility prediction, experimental data were modelled using four empirical density-based models: Chrastil, del Valle and Aguilera, Adachi and Lu, and Sparks et al. models. In general, all the models were able to predict the solubility of Christia vespertilionis oil in supercritical carbon dioxide. The best fitting showed that Adachi and Lu model gave the best correlation with the lowest %AARD value of 1.61%.

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Experimental and thermodynamic modeling decitabine anti cancer drug solubility in supercritical carbon dioxide

Scientific Reports ◽

10.1038/s41598-020-80399-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Mahboubeh Pishnamazi ◽

Samyar Zabihi ◽

Sahar Jamshidian ◽

Fatemeh Borousan ◽

Ali Zeinolabedini Hezave ◽

...

Keyword(s):

Carbon Dioxide ◽

Supercritical Carbon Dioxide ◽

Solubility Data ◽

Effect Of Temperature ◽

Cancer Drug ◽

Direct Relation ◽

Anti Cancer ◽

Temperature And Pressure ◽

The Cross ◽

Supercritical Carbon

AbstractDesign and development of efficient processes for continuous manufacturing of solid dosage oral formulations is of crucial importance for pharmaceutical industry in order to implement the Quality-by-Design paradigm. Supercritical solvent-based manufacturing can be utilized in pharmaceutical processing owing to its inherent operational advantages. However, in order to evaluate the possibility of supercritical processing for a particular medicine, solubility measurement needs to be carried out prior to process design. The current work reports a systematic solubility analysis on decitabine as an anti-cancer medicine. The solvent is supercritical carbon dioxide at different conditions (temperatures and pressures), while gravimetric technique is used to obtain the solubility data for decitabine. The results indicated that the solubility of decitabine varies between 2.84 × 10–05 and 1.07 × 10–03 mol fraction depending on the temperature and pressure. In the experiments, temperature and pressure varied between 308–338 K and 12–40 MPa, respectively. The solubility of decitabine was plotted against temperature and pressure, and it turned out that the solubility had direct relation with the pressure due to the effect of pressure on solvating power of solvent. The effect of temperature on solubility was shown to be dependent on the cross-over pressure. Below the cross-over pressure, there is a reverse relation between temperature and solubility, while a direct relation was observed above the cross-over pressure (16 MPa). Theoretical study was carried out to correlate the solubility data using several thermodynamic-based models. The fitting and model calibration indicated that the examined models were of linear nature and capable to predict the measured decitabine solubilities with the highest average absolute relative deviation percent (AARD %) of 8.9%.

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