scholarly journals Short-term oestrous synchronisation protocol following single fixed-time artificial insemination and natural mating as alternative to long-term protocol in dairy goats

2020 ◽  
Vol 192 ◽  
pp. 106207
Author(s):  
D.L.M. Gore ◽  
J.N. Mburu ◽  
T.O. Okeno ◽  
T.K. Muasya
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Dairy Goats ◽  
Short Term ◽  
Natural Mating

scholarly journals Fertility rate of short-term progestagen pretreated ewes in relation to breed: A field study

2018 ◽  
Vol 68 (1) ◽  
pp. 35
Author(s):  
S. PAPADOPOULOS ◽  
C. DELIGIANNIS ◽  
E. K. THEODOSIADOU ◽  
D. KANTAS ◽  
TH. LAINAS ◽  
...  
Keyword(s):  
Field Study ◽  
Artificial Insemination ◽  
Transition Period ◽  
Fertility Rate ◽  
Short Term ◽  
Serum Progesterone ◽  
Blood Samples ◽  
The Common ◽  
Natural Mating

The effect of short-term instead of long-term progestagen treatment on fertility of Karagouniko and Chios ewes, after natural mating or artificial insemination, was investigated. Two experiments were performed during the transition period from anοestrous to the breeding season. In the 1st experiment (natural mating, NM), Karagouniko and Chios ewes were randomly allocated into 3 groups, that were KLM (long-term progestagen treatment; n=35), KSM (short-term progestagen treatment; n=34), KSP (short-term progestagen treatment; prostaglandin; n=35) and CLM (n=40), CSM (n=35), CSP (n=38), respectively. In the 2nd experiment (intracervical artificial insemination, AI) Karagouniko and Chios ewes were randomly allocated into 3 groups, that were KLA (long-term progestagen treatment; AI at 54h; n=50), KSA1 (short-term progestagen treatment; AI at 54h; n=20), KSA2 (short-term progestagen treatment; AI at 48h; n=28) and CLA (n=40), CSA1 (n=16), CSA2 (n=20), respectively. At sponges’ removal (d0) all ewes received 400 IU eCG. Ten rams served NM, while for AI fresh diluted semen was used. Pregnancy diagnosis was performed, 45-50 days later. Ιn the 1st experiment, blood samples were collected, daily for 5 days, starting on d0, for serum progesterone assessment. Conception rate in Karagouniko ewes after NM was higher (P<0.05) in KSM (35.29%) compared to KLM (17.14%) group, but did not differ with KSP (28.57%) group, while after AI it was higher (P<0.05) in KLA (42.00%) or KSA1 (40.00%) compared to KSA2 (14.29%) group. In Chios ewes no significant differences were observed between groups either after NM [CLM (45.00%), CSM (36.84%), CSP (34.29%)] or after AI [CSA1 (50.00%), CSA2 (50.00%), CLA (45.00%)]. No significant differences were observed after NM or after AI in the litter size in both breeds. These results indicate that short-term progestagen treatment for oestrus synchronization could be applied in indigenous Greek sheep breeds, resulting in equal (Chios) or improved fertility (Karagouniko) than the common long-term one. Also, the fertility rate in ewes subjected to short-term progestagen treatment depends on the time of AI in relation to breed.

29 SHORT-TERM v. LONG-TERM PROGESTERONE PROTOCOL USING CERVICAL OR INTRAUTERINE FIXED-TIME INSEMINATION IN SHEEP

2010 ◽  
Vol 22 (1) ◽  
pp. 172
Author(s):  
A. Menchaca ◽  
M. Vilariño ◽  
E. Rubianes
Keyword(s):  
New York ◽  
Pregnancy Rate ◽  
Body Condition Score ◽  
Skim Milk ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Short Term ◽  
Condition Score ◽  
N 93

The short-term protocol with progesterone, prostaglandin F2α (PGF2α), and eCG is used to control follicular dynamics and luteal activity synchronizing the ovulation for fixed-time AI in sheep. The objective of this experiment was to compare the pregnancy rate obtained with short-term protocol (6 d) and long-term protocol (14 d) using cervical or intrauterine fixed-time AI in sheep. Three hundred fifty-two Merino ewes with a body condition score of 2.9 ± 0.3 (mean ± SD; scale 0 to 5) were used during the breeding season (April, 33S, Uruguay). All the females received a CIDR-G (0.3 g of progesterone, InterAg, Hamilton, New Zealand) for 6 d (short-term protocol; n = 178) or 14 d (long-term protocol, n = 174). One imdose of eCG (300 IU, Novormon, Syntex, BA, Argentina) was given at the moment of device withdrawal for the both protocols, and one imdose of PGF2α (10 mg of dinoprost, Lutalyse, Pfizer, New York, NY, USA) was given at the end of the short-term protocol to ensure luteolysis. Cervical AI (short-term protocol, n = 85; long-term protocol, n = 104) or intrauterine AI (short-term protocol, n = 93; long-term protocol, n = 70) was performed 48 or 54 h after device withdrawal, using 200 × 106 or 100 × 106 spermatozoa per ewe, respectively. Fresh semen was extended in UHT skim milk (1000 × 106 spermatozoa mL-1) and used within 1 h of collection. Estrus was recorded twice a day for 4 days after device withdrawal using vasectomized males. Pregnancy diagnosis was performed by transrectal ultrasonography 40 d after AI (5.0 MHz, Aloka, Tokyo, Japan). Logistic regression was used to analyze the effect of the treatment (P < 0.05), the AI technique (P < 0.05), and their interaction (P = NS). Pregnancy rate was higher for the short-term than for the long-term protocol, and for intrauterine than for cervical AI (Table 1). The highest pregnancy rate was achieved with short-term protocol using intrauterine AI (54.8%, 51/93), and the lowest response was obtained with long-term protocol using cervical AI (33.7%, 35/104; P < 0.05). These data were not different from data of short-term protocol using cervical AI or long-term protocol using intrauterine AI (42.4%, 36/85; and 44.3% 31/70, respectively). Ewes in estrus/treated ewes was not different among short-term and long-term protocols (83.7%, 149/178; and 82.8%, 144/174, respectively; P = NS). In summary, regardless of insemination technique, short-term protocol of 6 d enhances pregnancy rate in fixed-time AI programs in sheep. Table 1.Main effects of short-term (6 d) v. long-term (14 d) protocol using cervical or intrauterine fixed-time AI on pregnancy rate in sheep Financially supported by Pfizer, SP, Brazil.

Factors affecting reproductive performance of white-tailed deer subjected to fixed-time artificial insemination or natural mating

2013 ◽  
Vol 25 (3) ◽  
pp. 581 ◽  
Author(s):  
Miguel Mellado ◽  
Claudia G. Orta ◽  
Eloy A. Lozano ◽  
Jose E. García ◽  
Francisco G. Veliz ◽  
...  
Keyword(s):  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Arid Environment ◽  
Factors Affecting ◽  
Litter Weight ◽  
Subsequent Period ◽  
Natural Mating ◽  
Game Hunting

The objectives of this study were to examine the effects of several factors affecting fawning rate, litter size, litter weight and neonatal fawn mortality in white-tailed deer inseminated either transcervically or by means of laparoscopy. Oestrus synchronisation with a controlled internal drug release (CIDR)-based protocol and fixed-time artificial insemination (FTAI) was conducted in 130 white-tailed deer (Odocoileus virginianus texanus) during three reproductive seasons (2007–2009; 271 services) in a game-hunting ranch in a hot–arid environment (26°4′ N, 101°25′ W). Ninety additional non-treated does were exposed to bucks for natural mating. Fawning rate did not differ between AI methods (40.0 vs 45.0% for transcervical and laparoscopic AI, respectively). Overall fawning rate (proportion of all does fawning after FTAI and a subsequent period of buck exposure) did not differ between transcervical (89.5%), laparoscopic (80.3%) or natural (88.9%) insemination. Litter size per fawning doe was higher (P&lt;0.05) in naturally-served does (1.65±0.48) than in transcervically-inseminated does (1.40±0.51) or in laparoscopically-inseminated does (1.48±0.50). The main conclusion was that no enhancement of fawning rate or litter size occurred as a result of intrauterine deposition of semen by laparoscopy compared with the transcervical insemination technique.

Bromocriptine treatment of periparturient goats: long-term suppression of prolactin and lack of effect on lactation

1993 ◽  
Vol 60 (3) ◽  
pp. 307-317 ◽  
Author(s):  
Isabel A. Forsyth ◽  
Philip D. Lee
Keyword(s):  
Milk Yield ◽  
Post Partum ◽  
Milk Composition ◽  
Prolactin Secretion ◽  
Plasma Prolactin ◽  
Dairy Goats ◽  
Plasma Growth Hormone ◽  
Short Term ◽  
And Control

SummaryBritish Saanen dairy goats (n = 10) were treated with bromocriptine or vehicle from day 147 of pregnancy to day 4 post partum, a treatment duration of 8·8±1·7 d (mean ± SEM). The periparturient prolactin surge was abolished by this treatment, but there were no significant effects on plasma growth hormone or insulin concentrations. Lactogenesis was delayed in the bromocriptine-treated goats, milk yields being significantly depressed (P < 0·01) for the first week of lactation. Yields had recovered to control values by day 10 when prolactin concentrations were still significantly depressed. Mammary gland biopsies were taken on day 4 post partum from five animals in each group. Using this tissue, no significant differences could be shown in mammary morphology or DNA synthesis, but the RNA:DNA ratio was significantly reduced (P < 0·05). After week 1, there were no significant differences between bromocriptine-treated and control goats in milk yield, milk composition, udder volume, time of peak yield or persistence. The goats given short-term bromocriptine treatment at parturition showed prolonged effects on prolactin secretion, their seasonal prolactin rise being severely blunted (P < 0·001). A normal lactation is therefore not prevented in goats by a delay in lactogenesis, suppression of prolactin at parturition or the resulting prolonged depression of circulating prolactin. Goats in established lactation given bromocriptine for 8 d showed, by contrast, a rapid recovery of plasma prolactin concentrations within 5 d post treatment. Milk yield declined significantly (P < 0·03) compared with pretreatment values during and for 1 week after bromocriptine but then began to recover, with no significant change in vehicle-treated goats.

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