Interesting static quenching of fluorescent hydrogel caused by immobilization of Cd2+ ions within interstitial morphology

2021 ◽  
Vol 331 ◽  
pp. 129419
Author(s):  
Nira Parshi ◽  
Dipika Pan ◽  
Biswaji Jana ◽  
Jhuma Ganguly
Keyword(s):  
2021 ◽  
pp. 174751982199306
Author(s):  
Ya Gan ◽  
Ning Bai ◽  
Xitong Li ◽  
Shuiting Gao ◽  
Ruiyong Wang

The interactions between radicicol and four proteins (catalase, trypsin, pepsin, and human serum protein) are investigated by spectroscopic techniques and molecular docking. A static quenching process is confirmed. The binding constant value between radicicol and human serum protein is the largest among the four proteins. Results reveal changes in the micro-environment of the protein by the addition of radicicol. It is found that radicicol shows an inhibitory effect on the activity of proteins (catalase, trypsin, and pepsin). Molecular docking results are consistent with the thermodynamic experimental results. This work provides clues to the elucidation of the mechanisms of the interactions between radicicol and proteins.


2017 ◽  
Vol 71 (11) ◽  
pp. 2512-2518 ◽  
Author(s):  
Ryan P. Ferrie ◽  
Gregory E. Hewitt ◽  
Bruce D. Anderson

Fluorescence quenching was used to investigate the interaction of six fluoroquinolones with humic acid. Static quenching was observed for the binding of ciprofloxacin, enoxacin, fleroxacin, levofloxacin, norfloxacin, and ofloxacin to humic acid. The equilibrium binding constants were found from Stern–Volmer plots of the data. The quenching experiments were repeated over a temperature range of 25–45 ℃ and van’t Hoff plots were generated. From these linear plots, thermodynamic values were calculated for Δ H, Δ G, and Δ S for each of the fluoroquinolones. The equilibrium binding constants were found to be <1 for all the antibiotics studied. The calculated ΔH values were all negative and ranged from −9.5 to −27.6 kJ/mol. The high water solubility of the antibiotics and low ΔH of binding suggests that the antibiotics will be transported easily through the environment. Finally, whether the fluoroquinolones are in a protonated, deprotonated, or partially protonated state is found to correlate to the strength of binding to humic acid.


The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1227-1235 ◽  
Author(s):  
Xiao-Man Wu ◽  
Jun-Hua Zhang ◽  
Zi-Shuo Feng ◽  
Wen-Xin Chen ◽  
Fei Zhang ◽  
...  

A “turn-off” fluorescent sensor based on glutathione-stabilized copper nanoclusters (GSH-Cu NCs) was prepared for the sensitive detection of RFP via a static quenching strategy caused by the inner filter effect (IFE).


2019 ◽  
Vol 3 (12) ◽  
pp. 2751-2758 ◽  
Author(s):  
Jie Feng ◽  
Xiaoran Zhao ◽  
Wei Bian ◽  
Xinjing Tang

Highly fluorescent N-dots are rationally designed and synthesized through microwave-assisted reaction of 2-azidoimidazole with glycerol crosslinker and are employed as an effective chemosensor for Ag+ analysis via the static quenching effect.


2015 ◽  
Vol 119 (51) ◽  
pp. 28544-28550 ◽  
Author(s):  
Yue Li ◽  
Kun Liu ◽  
Wen-Juan Li ◽  
An Guo ◽  
Fang-Yao Zhao ◽  
...  

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