Highly selective electrochemiluminescence aptasensor coupled with mesoporous Fe3O4@Cu@Cu2O as co-reaction accelerator for ATP assay based on target-triggered emitter release

Abstract Background This study aimed to compare the use of photon-initiated photoacoustic streaming (PIPS) and conventional needle irrigation (CNI) in conjunction with different concentrations of sodium hypochlorite (NaOCl) to remove Enterococcus faecalis (E. faecalis) suspended bacteria and biofilms from root canal systems with different diameters or tapers. Methods Artificial root canal samples (n = 480) were randomly divided into three groups (n = 160/group). The canals were prepared to fit file sizes #10/.02, #25/.02, or #25/.06. The size #10/.02 group was incubated for seven days. The size #25/.02 or #25/.06 group was incubated for 2 days. A stable biological model of E. faecalis infection was established. The root canals were washed with distilled water or with 1%, 2%, or 5.25% NaOCl combined with CNI or PIPS. Bacterial suspensions and biofilms were assessed using an ATP assay kit and fluorescence microscopy. Image-Pro Plus was used to analyse the average fluorescence intensity to determine the most suitable root canal irrigation solution. Results In the CNI and PIPS groups, the ATP value of the 5.25% NaOCl subgroup was the lowest, followed by that of the 2% and 1% NaOCl subgroups. The ATP value of the distilled water subgroup was the highest (P < 0.05). When the root canal taper was 0.02, the ATP value of the #10/.02 + PIPS group was significantly lower than that of the #25/.02 + CNI group (P < 0.05). The average fluorescence intensity of the #10/.02 + PIPS group was lower than that of the #25/.02 + CNI group (P < 0.05). When the apical diameter was #25, the ATP value of the 0.02 taper in the PIPS group was lower than that of the 0.06 taper in the CNI group (P < 0.05), and the average fluorescence intensity of the 0.02 taper + PIPS group was lower than that of the 0.06 taper + CNI group (P < 0.05). PIPS combined with 2% and 5.25% NaOCl effectively improved the long-term antibacterial effect after irrigation and re-culture for 6 h. Conclusions Compared with CNI, PIPS has greater ability to remove bacteria in root canals with a small preparation diameter and a small taper. PIPS with 2% and 5.25% NaOCl exhibited superior antibacterial and bacteriostatic effects.

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A technique for the processing of blood samples for subsequent assay of ATP, and an investigation of the method of standardization of the firefly-luciferase ATP assay

Clinica Chimica Acta ◽

10.1016/0009-8981(66)90021-0 ◽

1966 ◽

Vol 14 (6) ◽

pp. 836-839 ◽

Cited By ~ 6

Author(s):

G.J. Brewer ◽

C.A. Knutsen

Keyword(s):

Firefly Luciferase ◽

Blood Samples ◽

Atp Assay

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Mass Treatment of Flooded Archival Materials by Gamma Radiation

Restaurator International Journal for the Preservation of Library and Archival Material ◽

10.1515/res-2021-0007 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Matteo Montanari ◽

Sara Pipponzi ◽

Pietro Livi ◽

Antonio Prodi

Keyword(s):

Gamma Radiation ◽

Gamma Irradiation ◽

Culture Method ◽

Test Species ◽

Atp Assay ◽

Recovery Processes ◽

Mycelia Growth ◽

Thermal Drying ◽

Radiation Treatments ◽

Main Components

Abstract This work describes mass recovery processes of flooded archival materials at industrial scale. The presence of fungi on paper represents a threat to the integrity of the document because they degrade cellulose, one of the main components of paper. Gamma radiation treatments are investigated as mass disinfection agents for their high penetrating power, speed of treatment, and absence of risk due to chemical residuals. We compared two different recovery processes: thermal drying followed by gamma irradiation and gamma irradiation followed by thermal drying. Both these processes were conducted simultaneously on naturally contaminated archival items and on paper specimens artificially contaminated with species test. Efficacy was assessed by culture method and ATP assay, right after the treatments and after four years of storage at room temperature. Coupling gamma irradiation with a drying step with dry heat at 55–60 °C reduces the fungal loads on natural items up to levels close to the detection limits, and the reduction is maintained after four years. On artificial specimens, spore germination is completely inhibited, mycelia growth is also highly affected, but the melanised test species appear to be more resistant. A synergistic effect between gamma irradiation, water content, and thermal drying is highlighted in this paper.

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The MTS vs. the ATP assay for in vitro chemosensitivity testing of primary glioma tumour culture

Neuropathology and Applied Neurobiology ◽

10.1111/j.1365-2990.2010.01096.x ◽

2010 ◽

Vol 36 (6) ◽

pp. 564-567 ◽

Cited By ~ 4

Author(s):

T. P. Dawson ◽

R. V. Iyer ◽

R. W. Lea ◽

P. Roberts ◽

F. Harris ◽

...

Keyword(s):

Chemosensitivity Testing ◽

Atp Assay ◽

In Vitro Chemosensitivity ◽

Primary Glioma

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Improvements of ATP Assay as a Substitute for the CFU Method in Estimating Viable Cell Count for BCG/rBCG Vaccine Preparations

Journal of Vaccines & Vaccination ◽

10.4172/2157-7560.1000309 ◽

2016 ◽

Vol 07 (01) ◽

Cited By ~ 2

Author(s):

Tom H Jin ◽

Tianli Qu

Keyword(s):

Cell Count ◽

Viable Cell ◽

Viable Cell Count ◽

Atp Assay

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Rapid Enumeration of Airborne Microorganisms by Bioluminescent ATP Assay

Physical Methods for Microorganisms Detection ◽

10.1201/9781351075596-6 ◽

2018 ◽

pp. 111-127

Author(s):

Jillian S. Deans ◽

I. W. Stewart ◽

T. T. Salusbury

Keyword(s):

Airborne Microorganisms ◽

Atp Assay ◽

Rapid Enumeration

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ATP assay for rapid onboard testing to detect living microorganisms in Ballast Water

10.24868/bwtc6.2017.012 ◽

2017 ◽

Author(s):

Seungshic Yum ◽

Bong Gil Hyun ◽

Kitae Rhie ◽

Kyoungsoon Shin

Keyword(s):

Water Management ◽

Water Treatment ◽

Cell Volume ◽

Ballast Water ◽

Treatment System ◽

Diatom Species ◽

Water Treatment System ◽

Atp Assay ◽

Ballast Water Treatment ◽

Ballast Water Management

Rapid and simple analytical methods for viable microorganism detection in ballast water are required to evaluate the efficiency of ballast water treatment system. During the course of systematic investigation of the cytotoxicity and apoptosis assays, it was found that the adenosine triphosphate (ATP) and luminescence based cell viability assay, in other word, an ATP assay was the most sensitive and applicable to ballast water management (BWM). The assay was applied to cultured microalgae samples, and it could detect the existence of 5 viable cells in 100 μl. Comparably low luminescent values were detected in two cultured diatom species than in cultured dinoflagellates. This result might be caused by the small cell volume in diatom species. Following a regression model between ATP concentration and cell volume, an ATP guideline (876 – 109246 relative luminescence units: RLU) was developed for the evaluation of treated ballast water. ATP assay was also applied to the evaluation of ballast water treatment system (BWTS). The luminescence value which obtained from the ATP assay also showed a good correlation with the presence of living natural plankton cells with comparably low luminescence values than the cultured species. The low ATP concentration in natural plankton cells may reflect a decline in their biological activity because of extended exposure to dark conditions. ATP assay could be a suitable method for the monitoring of ballast water management compliance even though the results of this study need further validation.

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