scholarly journals TGF-β1 Negatively Regulates the Number and Function of Hematopoietic Stem Cells

2018 ◽  
Vol 11 (1) ◽  
pp. 274-287 ◽  
Author(s):  
Xiaofang Wang ◽  
Fang Dong ◽  
Sen Zhang ◽  
Wanzhu Yang ◽  
Wenying Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
And Function ◽  
Tgf Β1

Optimization of cytapheresis products' storage for CD34+ hematopoietic stem cells viability and function maintenance

Cytotherapy ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. S78
Author(s):  
N. Lombion ◽  
C. Gouat ◽  
D. Tramalloni ◽  
V. Lapierre ◽  
B.S. Marteyn
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
And Function

scholarly journals Serine/Threonine Pim Kinases Play an Important Role in Maintaining the Number and Function of Hematopoietic Stem Cells

2012 ◽  
Vol 18 (2) ◽  
pp. S374
Author(s):  
N. An ◽  
Y.-W. Lin ◽  
A. Liu ◽  
M. Lilly ◽  
S. Mahajan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
Pim Kinases ◽  
And Function

scholarly journals The chemokine GROβ mobilizes early hematopoietic stem cells characterized by enhanced homing and engraftment

Blood ◽  
2007 ◽  
Vol 110 (3) ◽  
pp. 860-869 ◽  
Author(s):  
Seiji Fukuda ◽  
Huimin Bian ◽  
Andrew G. King ◽  
Louis M. Pelus
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
Platelet Recovery ◽  
And Function ◽  
Stimulating Factor ◽  
Cxcr4 Axis

Abstract Mobilized peripheral blood hematopoietic stem cells (PBSCs) demonstrate accelerated engraftment compared with bone marrow; however, mechanisms responsible for enhanced engraftment remain unknown. PBSCs mobilized by GROβ (GROβΔ4/CXCL2Δ4) or the combination of GROβΔ4 plus granulocyte colony-stimulating factor (G-CSF) restore neutrophil and platelet recovery faster than G-CSF–mobilized PBSCs. To determine mechanisms responsible for faster hematopoietic recovery, we characterized immunophenotype and function of the GROβ-mobilized grafts. PBSCs mobilized by GROβΔ4 alone or with G-CSF contained significantly more Sca-1+-c-kit+-lineage− (SKL) cells and more primitive CD34−-SKL cells compared with cells mobilized by G-CSF and demonstrated superior competitive long-term repopulation activity, which continued to increase in secondary and tertiary recipients. GROβΔ4-mobilized SKL cells adhered better to VCAM-1+ endothelial cells compared with G-CSF–mobilized cells. GROβΔ4-mobilized PBSCs did not migrate well to the chemokine stromal derived factor (SDF)-1α in vitro that was associated with higher CD26 expression. However, GROβΔ4-mobilized SKL and c-Kit+ lineage− (KL) cells homed more efficiently to marrow in vivo, which was not affected by selective CXCR4 and CD26 antagonists. These data suggest that GROβΔ4-mobilized PBSCs are superior in reconstituting long-term hematopoiesis, which results from differential mobilization of early stem cells with enhanced homing and long-term repopulating capacity. In addition, homing and engraftment of GROβΔ4-mobilized cells is less dependent on the SDF-1α/CXCR4 axis.

Insights into signaling and function of hematopoietic stem cells at the single-cell level

2009 ◽  
Vol 16 (4) ◽  
pp. 255-258 ◽  
Author(s):  
Satoshi Yamazaki ◽  
Hiromitsu Nakauchi
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Hematopoietic Stem ◽  
And Function

scholarly journals FIP200 is required for the cell-autonomous maintenance of fetal hematopoietic stem cells

Blood ◽  
2010 ◽  
Vol 116 (23) ◽  
pp. 4806-4814 ◽  
Author(s):  
Fei Liu ◽  
Jae Y. Lee ◽  
Huijun Wei ◽  
Osamu Tanabe ◽  
James D. Engel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Cells ◽  
Cellular Functions ◽  
Interacting Protein ◽  
Hematopoietic Stem ◽  
Conditional Deletion ◽  
Perinatal Lethality ◽  
Fetal Hematopoiesis ◽  
And Function

Abstract Little is known about whether autophagic mechanisms are active in hematopoietic stem cells (HSCs) or how they are regulated. FIP200 (200-kDa FAK-family interacting protein) plays important roles in mammalian autophagy and other cellular functions, but its role in hematopoietic cells has not been examined. Here we show that conditional deletion of FIP200 in hematopoietic cells leads to perinatal lethality and severe anemia. FIP200 was cell-autonomously required for the maintenance and function of fetal HSCs. FIP200-deficient HSC were unable to reconstitute lethally irradiated recipients. FIP200 ablation did not result in increased HSC apoptosis, but it did increase the rate of HSC proliferation. Consistent with an essential role for FIP200 in autophagy, FIP200-null fetal HSCs exhibited both increased mitochondrial mass and reactive oxygen species. These data identify FIP200 as a key intrinsic regulator of fetal HSCs and implicate a potential role for autophagy in the maintenance of fetal hematopoiesis and HSCs.

scholarly journals Expression and Function of Murine Receptor Tyrosine Kinases, TIE and TEK, in Hematopoietic Stem Cells

Blood ◽  
1997 ◽  
Vol 89 (12) ◽  
pp. 4317-4326 ◽  
Author(s):  
Michihiro Yano ◽  
Atsushi Iwama ◽  
Hitoshi Nishio ◽  
Junko Suda ◽  
Goro Takada ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Tyrosine Kinases ◽  
Receptor Tyrosine Kinases ◽  
Stem Cell Marker ◽  
Proliferative Potential ◽  
Hematopoietic Stem ◽  
And Function

Abstract Two highly related receptor tyrosine kinases, TIE and TEK, comprise a family of endothelial cell-specific kinase. We established monoclonal antibodies against them and performed detailed analyses on their expression and function in murine hematopoietic stem cells (HSCs). TIE and TEK were expressed on 23.7% and 33.3% of lineage marker-negative, c-Kit+ and Sca-1+ (Lin− c-Kit+ Sca-1+) HSCs that contain the majority of day-12 colony-forming units-spleen (CFU-S) and long-term reconstituting cells, but not committed progenitor cells. Lin− c-Kit+ Sca-1+ cells were further divided by the expression of TIE and TEK. TIE+ and TEK+ HSCs as well as each negative counterpart contained high proliferative potential colony-forming cells and differentiated into lymphoid and myeloid progenies both in vitro and in vivo. However, day-12 CFU-S were enriched in TIE+ and TEK+ HSCs. Our findings define TIE and TEK as novel stem cell marker antigens that segregate day-12 CFU-S, and provide evidence of novel signaling pathways that are involved in the functional regulation of HSCs at a specific stage of differentiation, particularly of day-12 CFU-S.

scholarly journals Critical Role of Jak2 in the Maintenance and Function of Adult Hematopoietic Stem Cells

Stem Cells ◽  
2014 ◽  
Vol 32 (7) ◽  
pp. 1878-1889 ◽  
Author(s):  
Hajime Akada ◽  
Saeko Akada ◽  
Robert E. Hutchison ◽  
Kazuhito Sakamoto ◽  
Kay-Uwe Wagner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Critical Role ◽  
Hematopoietic Stem ◽  
And Function
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