Fifteen-year no tillage of a Mollisol with residue retention indirectly affects topsoil bacterial community by altering soil properties

Soil physiochemical properties are regulated by cropping practices, but little is known about how tillage influences soil microbial community diversity and functions. Here, we assessed soil bacterial community assembly and functional profiles in relation to tillage. Soils, collected in 2018 from a 17-year field experiment in northwestern China, were analyzed using high-throughput sequencing and the PICRUSt approach. The taxonomic diversity of bacterial communities was dominated primarily by the phyla Proteobacteria (32–56%), Bacteroidetes (12–33%), and Actinobacteria (17–27%). Alpha diversity (Chao1, Shannon, Simpson, and operational taxonomic unit (OTU) richness) was highest under no-tillage with crop residue removed (NT). Crop residue retention on the soil surface (NTS) or incorporated into soil (TS) promoted the abundance of Proteobacteria by 16 to 74% as compared to conventional tillage (T). Tillage practices mainly affected the pathways of soil metabolism, genetic information processing, and environmental information processing. Soil organic C and NH4–N were the principal contributors to the diversity and composition of soil microbiota, whereas soil pH, total nitrogen, total P, and moisture had little effect. Our results suggest that long-term conservation practices with no-tillage and crop residue retention shape soil bacterial community composition through modifying soil physicochemical properties and promoting the metabolic function of soil microbiomes.

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Corrigendum to: Conservation agriculture effects on soil properties and crop productivity in a semiarid region of India

Soil Research ◽

10.1071/sr18145_co ◽

2019 ◽

Vol 57 (2) ◽

pp. 200 ◽

Cited By ~ 1

Author(s):

J. Somasundaram ◽

M. Salikram ◽

N. K. Sinha ◽

M. Mohanty ◽

R. S. Chaudhary ◽

...

Keyword(s):

Soil Properties ◽

Management Practices ◽

Crop Yields ◽

Cropping Systems ◽

Soil Layer ◽

Conservation Agriculture ◽

Semiarid Region ◽

Reduced Tillage ◽

Organic C ◽

Residue Retention

Conservation agriculture (CA) including reduced or no-tillage and crop residue retention, is known to be a self–sustainable system as well as an alternative to residue burning. The present study evaluated the effect of reduced tillage coupled with residue retention under different cropping systems on soil properties and crop yields in a Vertisol of a semiarid region of central India. Two tillage systems – conventional tillage (CT) with residue removed, and reduced tillage (RT) with residue retained – and six major cropping systems of this region were examined after 3 years of experimentation. Results demonstrated that soil moisture content, mean weight diameter, percent water stable aggregates (>0.25mm) for the 0–15cm soil layer were significantly (Pmoderately labile>less labile. At the 0–15cm depth, the contributions of moderately labile, less labile and non-labile C fractions to total organic C were 39.3%, 10.3% and 50.4% respectively in RT and corresponding values for CT were 38.9%, 11.7% and 49.4%. Significant differences in different C fractions were observed between RT and CT. Soil microbial biomass C concentration was significantly higher in RT than CT at 0–15cm depth. The maize–chickpea cropping system had significantly (P–1 followed by soybean+pigeon pea (2:1) intercropping (3.50 t ha–1) and soybean–wheat cropping systems (2.97 t ha–1). Thus, CA practices could be sustainable management practices for improving soil health and crop yields of rainfed Vertisols in these semiarid regions.

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Four decades of continuously applied tillage or no‐tillage on soil properties and soil morphology

age ◽

10.1002/agg2.20195 ◽

2021 ◽

Vol 4 (3) ◽

Author(s):

Silvia Mestelan ◽

Neil Smeck ◽

Christine Sprunger ◽

Ashly Dyck ◽

Warren Dick

Keyword(s):

Soil Properties ◽

No Tillage ◽

Soil Morphology

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Bacillus amyloliquefaciens FH-1 significantly affects cucumber seedlings and the rhizosphere bacterial community but not soil

Scientific Reports ◽

10.1038/s41598-021-91399-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jingjing Wang ◽

Song Xu ◽

Rong Yang ◽

Wei Zhao ◽

Dan Zhu ◽

...

Keyword(s):

Bacterial Community ◽

Soil Properties ◽

Bacillus Amyloliquefaciens ◽

Soil Conditions ◽

Plant Growth Promoting Bacteria ◽

Linear Discriminant ◽

Cucumber Seedlings ◽

Ecological Roles ◽

Plant Growth Promoting ◽

Rhizosphere Bacterial Community

AbstractPlant growth-promoting bacteria (PGPB) inoculants have been applied worldwide. However, the ecological roles of PGPB under different soil conditions are still not well understood. The present study aimed to explore the ecological roles of Bacillus amyloliquefaciens FH-1 (FH) on cucumber seedlings, rhizosphere soil properties, and the bacterial community in pot experiments. The results showed that FH had significant effects on cucumber seedlings and the rhizosphere bacterial community but not on soil properties. The FH promoted cucumber seedlings growth, reduced the rhizosphere bacterial diversity, increased Proteobacteria, and decreased Acidobacteria. Linear discriminant analysis (LDA) effect size (LEfSe) revealed that FH enriched two taxa (GKS2_174 and Nannocystaceae) and inhibited 18 taxa (mainly Acidobacteria, Actinobacteria, BRC1, Chloroflexi, Plantctomycetes, and Verrucomicrobia). Co-occurrence network analysis demonstrated that FH increased bacteria-bacteria interactions and that Bacillus (genus of FH) had few interactions with the enriched and inhibited taxa. This might indicate that FH does not directly affect the enriched and inhibited taxa. Correlation analysis results displayed that cucumber seedlings’ weight and height/length (except root length) were significantly correlated with the 18 inhibited taxa and the enriched taxa Nannocystaceae. It was speculated that FH might promote cucumber seedling growth by indirectly enriching Nannocystaceae and inhibiting some taxa from Acidobacteria, Actinobacteria, BRC1, Chloroflexi, Plantctomycetes, and Verrucomicrobia.

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