scholarly journals Directed Computational Evolution of Quorum-Quenching Lactonases from the Amidohydrolase Superfamily

Structure ◽  
2020 ◽  
Vol 28 (6) ◽  
pp. 635-642.e3
Author(s):  
Maybelle Kho Go ◽  
Li Na Zhao ◽  
Bo Xue ◽  
Shreyas Supekar ◽  
Robert C. Robinson ◽  
...  
Keyword(s):  
Quorum Quenching ◽  
Amidohydrolase Superfamily

Directed Evolution of a Quorum-Quenching Lactonase fromMycobacterium aviumsubsp.paratuberculosisK-10 in the Amidohydrolase Superfamily

Biochemistry ◽  
2009 ◽  
Vol 48 (20) ◽  
pp. 4344-4353 ◽  
Author(s):  
Jeng Yeong Chow ◽  
Long Wu ◽  
Wen Shan Yew
Keyword(s):  
Directed Evolution ◽  
Quorum Quenching ◽  
Amidohydrolase Superfamily

scholarly journals Directed Evolution of a Thermostable Quorum-quenching Lactonase from the Amidohydrolase Superfamily

2010 ◽  
Vol 285 (52) ◽  
pp. 40911-40920 ◽  
Author(s):  
Jeng Yeong Chow ◽  
Bo Xue ◽  
Kang Hao Lee ◽  
Alvin Tung ◽  
Long Wu ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Quorum Quenching ◽  
Amidohydrolase Superfamily

Antibiotics Application Strategies to Control Biofilm Formation in Pathogenic Bacteria

2020 ◽  
Vol 21 (4) ◽  
pp. 270-286 ◽  
Author(s):  
Fazlurrahman Khan ◽  
Dung T.N. Pham ◽  
Sandra F. Oloketuyi ◽  
Young-Mog Kim
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Extracellular Polymeric Substances ◽  
Quorum Quenching ◽  
Resistance Mechanisms ◽  
Bacterial Biofilm ◽  
Bacterial Cells ◽  
High Concentration ◽  
Biofilm Inhibition ◽  
Abiotic Surfaces

Background: The establishment of a biofilm by most pathogenic bacteria has been known as one of the resistance mechanisms against antibiotics. A biofilm is a structural component where the bacterial community adheres to the biotic or abiotic surfaces by the help of Extracellular Polymeric Substances (EPS) produced by bacterial cells. The biofilm matrix possesses the ability to resist several adverse environmental factors, including the effect of antibiotics. Therefore, the resistance of bacterial biofilm-forming cells could be increased up to 1000 times than the planktonic cells, hence requiring a significantly high concentration of antibiotics for treatment. Methods: Up to the present, several methodologies employing antibiotics as an anti-biofilm, antivirulence or quorum quenching agent have been developed for biofilm inhibition and eradication of a pre-formed mature biofilm. Results: Among the anti-biofilm strategies being tested, the sub-minimal inhibitory concentration of several antibiotics either alone or in combination has been shown to inhibit biofilm formation and down-regulate the production of virulence factors. The combinatorial strategies include (1) combination of multiple antibiotics, (2) combination of antibiotics with non-antibiotic agents and (3) loading of antibiotics onto a carrier. Conclusion: The present review paper describes the role of several antibiotics as biofilm inhibitors and also the alternative strategies adopted for applications in eradicating and inhibiting the formation of biofilm by pathogenic bacteria.

scholarly journals Anti-quorum quenching activity of methyl gallate isolated from galls of Guiera senegalensis J. F. Gmel (Combretaceae)

2019 ◽  
Vol 13 (16) ◽  
pp. 290-297 ◽  
Author(s):  
Ouedraogo Vincent ◽  
Alexandre Eric Djifaby Sombié Pierre ◽  
Compaoré Moussa ◽  
Kiendrébéogo Martin
Keyword(s):  
Quorum Quenching ◽  
Methyl Gallate ◽  
Guiera Senegalensis

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

Molecular docking, quorum quenching effect, antibiofilm activity and safety profile of silver-complexed sulfonamide on Pseudomonas aeruginosa

Biofouling ◽  
2021 ◽  
pp. 1-17
Author(s):  
Fallon dos Santos Siqueira ◽  
Camilla Filippi dos Santos Alves ◽  
Alencar Kolinski Machado ◽  
Josiéli Demétrio Siqueira ◽  
Thiago dos Santos ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Docking ◽  
Safety Profile ◽  
Quorum Quenching ◽  
Antibiofilm Activity ◽  
Quenching Effect

scholarly journals A single point mutation converts a glutaryl-7-aminocephalosporanic acid acylase into an N-acyl-homoserine lactone acylase

2021 ◽  
Author(s):  
Shereen A. Murugayah ◽  
Gary B. Evans ◽  
Joel D. A. Tyndall ◽  
Monica L. Gerth
Keyword(s):  
Single Point ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Site Directed Mutagenesis ◽  
Saturation Mutagenesis ◽  
Single Amino Acid ◽  
Single Point Mutation ◽  
Acyl Homoserine Lactone ◽  
Signalling Molecules ◽  
Single Amino Acid Residue

Abstract Objective To change the specificity of a glutaryl-7-aminocephalosporanic acid acylase (GCA) towards N-acyl homoserine lactones (AHLs; quorum sensing signalling molecules) by site-directed mutagenesis. Results Seven residues were identified by analysis of existing crystal structures as potential determinants of substrate specificity. Site-saturation mutagenesis libraries were created for each of the seven selected positions. High-throughput activity screening of each library identified two variants—Arg255Ala, Arg255Gly—with new activities towards N-acyl homoserine lactone substrates. Structural modelling of the Arg255Gly mutation suggests that the smaller side-chain of glycine (as compared to arginine in the wild-type enzyme) avoids a key clash with the acyl group of the N-acyl homoserine lactone substrate. Conclusions Mutation of a single amino acid residue successfully converted a GCA (with no detectable activity against AHLs) into an AHL acylase. This approach may be useful for further engineering of ‘quorum quenching’ enzymes.

scholarly journals Quorum-quenching activity of some Iranian medicinal plants

2021 ◽  
pp. 100882
Author(s):  
Farhad Moradi ◽  
Nahal Hadi
Keyword(s):  
Medicinal Plants ◽  
Quorum Quenching
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