Antibiotics Application Strategies to Control Biofilm Formation in Pathogenic Bacteria

Background: The establishment of a biofilm by most pathogenic bacteria has been known as one of the resistance mechanisms against antibiotics. A biofilm is a structural component where the bacterial community adheres to the biotic or abiotic surfaces by the help of Extracellular Polymeric Substances (EPS) produced by bacterial cells. The biofilm matrix possesses the ability to resist several adverse environmental factors, including the effect of antibiotics. Therefore, the resistance of bacterial biofilm-forming cells could be increased up to 1000 times than the planktonic cells, hence requiring a significantly high concentration of antibiotics for treatment. Methods: Up to the present, several methodologies employing antibiotics as an anti-biofilm, antivirulence or quorum quenching agent have been developed for biofilm inhibition and eradication of a pre-formed mature biofilm. Results: Among the anti-biofilm strategies being tested, the sub-minimal inhibitory concentration of several antibiotics either alone or in combination has been shown to inhibit biofilm formation and down-regulate the production of virulence factors. The combinatorial strategies include (1) combination of multiple antibiotics, (2) combination of antibiotics with non-antibiotic agents and (3) loading of antibiotics onto a carrier. Conclusion: The present review paper describes the role of several antibiotics as biofilm inhibitors and also the alternative strategies adopted for applications in eradicating and inhibiting the formation of biofilm by pathogenic bacteria.

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Application of Bacteriophages on Shiga Toxin-Producing Escherichia coli (STEC) Biofilm

Antibiotics ◽

10.3390/antibiotics10111423 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1423

Author(s):

Nicola Mangieri ◽

Roberto Foschino ◽

Claudia Picozzi

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Biofilm Formation ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Bacterial Cells ◽

Abiotic Surfaces ◽

Continuous Presence ◽

Different Strains ◽

Better Than

Shiga toxin-producing Escherichia coli are pathogenic bacteria able to form biofilms both on abiotic surfaces and on food, thus increasing risks for food consumers. Moreover, biofilms are difficult to remove and more resistant to antimicrobial agents compared to planktonic cells. Bacteriophages, natural predators of bacteria, can be used as an alternative to prevent biofilm formation or to remove pre-formed biofilm. In this work, four STEC able to produce biofilm were selected among 31 different strains and tested against single bacteriophages and two-phage cocktails. Results showed that our phages were able to reduce biofilm formation by 43.46% both when used as single phage preparation and as a cocktail formulation. Since one of the two cocktails had a slightly better performance, it was used to remove pre-existing biofilms. In this case, the phages were unable to destroy the biofilms and reduce the number of bacterial cells. Our data confirm that preventing biofilm formation in a food plant is better than trying to remove a preformed biofilm and the continuous presence of bacteriophages in the process environment could reduce the number of bacteria able to form biofilms and therefore improve the food safety.

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The phycobiota-derived proteins Pµ84 and Pµ19 suppress bacterial biofilm formation in gram-negative pathogens

10.5194/biofilms9-63 ◽

2020 ◽

Author(s):

Yuchen Han ◽

Wolfgang R. Streit ◽

Ines Krohn

Keyword(s):

Biofilm Formation ◽

Pathogenic Bacteria ◽

Bacterial Biofilm ◽

Klebsiella Pneumonia ◽

Biofilm Inhibition ◽

Marine Systems ◽

Microtiter Plates ◽

Small Proteins ◽

Ongoing Work ◽

Growth Promoting

Microalgae are typically found in freshwater and marine systems and they harbor a mostly a beneficial growth promoting microbiota. We have recently isolated several small proteins from the microbiomes of microalga (Scenedesmus quadricauda, Microasterias crux-melintensis, Chlorella saccherophilia) and have tested them for their role in either inhibition of biofilm formation and/or biofilm degradation. Thereby we have identified two candidate proteins that showed promising activities on biofilm inhibition and degradation. These proteins were designated P&#181;84 and P&#181;19 and strongly affected biofilm formation in several human- and plant-pathogenic bacteria. Recombinant and purified P&#181;84 and P&#181;19 were applied in biofilm assays in microtiter plates and reduced biofilms formed by Stenotrophomonas maltophilia, Pseudomonas aeruginosa and Klebsiella pneumonia. If expressed in the different hosts, biofilms were reduced by a factor of 40% and if applied as exogenous proteins, biofilms were reduced up to 20%. P&#181;84 application also resulted in a delayed biofilm formation and biofilm formation was affected by a factor of 17%. The microprotein P&#181;19 consist of 57 aa and P&#181;84 consists of 49 aa. Ongoing work elucidates the mechanism of P&#181;84 and P&#181;19 on the reduction of biofilm in order to achieve the optimal activity.

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Anti-Biofilm Properties Exhibited by Different Types of Monofloral Honey

Proceedings ◽

10.3390/proceedings2020066016 ◽

2021 ◽

Vol 66 (1) ◽

pp. 16

Author(s):

Filomena Nazzaro ◽

Florinda Fratianni ◽

Antonio d’Acierno ◽

Maria Neve Ombra ◽

Lucia Caputo ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Listeria Monocytogenes ◽

Biofilm Formation ◽

Inhibitory Action ◽

Bacterial Biofilm ◽

Bacterial Cells ◽

Biofilm Inhibition ◽

Tree Of Heaven ◽

Different Types

Our aim was to evaluate the susceptibility of bacterial biofilm formation and the metabolic changes occurring in the bacterial cells by the action of ivy, strawberry tree, lavender, sulla and tree of heaven monofloral honeys. Listeria monocytogenes was the most sensitive bacteria with percentages of biofilm inhibition up to 72.20%. Pseudomonas aeruginosa was less sensitive, but tree of heaven and sulla honey caused an inhibition of biofilm up to 40.41% and 35.85%, respectively. The tree of heaven honey acted on the P. aeruginosa metabolism (75.24%). Staphylococcus aureus, majorly resistant to the biofilm-inhibitory action of the honey, was more sensitive at the metabolic level (61.63% inhibition in the presence of the tree of heaven honey).

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Role of a Putative Polysaccharide Locus in Bordetella Biofilm Development

Journal of Bacteriology ◽

10.1128/jb.00953-06 ◽

2006 ◽

Vol 189 (3) ◽

pp. 750-760 ◽

Cited By ~ 82

Author(s):

Gina Parise ◽

Meenu Mishra ◽

Yoshikane Itoh ◽

Tony Romeo ◽

Rajendar Deora

Keyword(s):

Biofilm Formation ◽

Extracellular Polymeric Substances ◽

Glycosyl Hydrolase ◽

Bacterial Biofilm ◽

Abiotic Surfaces ◽

The Stability ◽

Biofilm Development ◽

Complex Architecture ◽

Microscopic Techniques

ABSTRACT Bordetellae are gram-negative bacteria that colonize the respiratory tracts of animals and humans. We and others have recently shown that these bacteria are capable of living as sessile communities known as biofilms on a number of abiotic surfaces. During the biofilm mode of existence, bacteria produce one or more extracellular polymeric substances that function, in part, to hold the cells together and to a surface. There is little information on either the constituents of the biofilm matrix or the genetic basis of biofilm development by Bordetella spp. By utilizing immunoblot assays and by enzymatic hydrolysis using dispersin B (DspB), a glycosyl hydrolase that specifically cleaves the polysaccharide poly-β-1,6-N-acetyl-d-glucosamine (poly-β-1,6-GlcNAc), we provide evidence for the production of poly-β-1,6-GlcNAc by various Bordetella species (Bordetella bronchiseptica, B. pertussis, and B. parapertussis) and its role in their biofilm development. We have investigated the role of a Bordetella locus, here designated bpsABCD, in biofilm formation. The bps (Bordetella polysaccharide) locus is homologous to several bacterial loci that are required for the production of poly-β-1,6-GlcNAc and have been implicated in bacterial biofilm formation. By utilizing multiple microscopic techniques to analyze biofilm formation under both static and hydrodynamic conditions, we demonstrate that the bps locus, although not essential at the initial stages of biofilm formation, contributes to the stability and the maintenance of the complex architecture of Bordetella biofilms.

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Antibiofilm activity of aminopropanol derivatives against Pseudomonas aeruginosa

Farmatsevtychnyi zhurnal ◽

10.32352/0367-3057.1.17.12 ◽

2018 ◽

pp. 93-100

Author(s):

D. M. Dudikova ◽

Z. S. Suvorova ◽

V. V. Nedashkivska ◽

A. O. Sharova ◽

M. L. Dronova ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Biofilm Formation ◽

Antimicrobial Agents ◽

Bacterial Biofilm ◽

Antibiofilm Activity ◽

Dependent Manner ◽

Inhibition Activity ◽

Chronic Infections ◽

High Concentration ◽

Biofilm Inhibition

Bacterial biofilm, particularly formed by Pseudomonas aeruginosa, are a cause of severe chronic infectious diseases. Bacteria within a biofilm are phenotypically more resistant to antibiotics and the macroorganism immune system, making it an important virulence factor for many microbes. The aminopropanol derivatives with adamantyl (KVM-97) and N-alkylaryl radicals (KVM-194, KVM-204, KVM-261, and KVM-262) were used as study object. The aim of this study was to investigate the antibiofilm activity of compounds on biofilm formation and on mature biofilm of P. aeruginosa. The effects of the aminopropanol derivatives on the biofilm mass were evaluated by using crystal violet assay. Ciprofloxacin, meropenem, ceftazidime, gentamicin were used as reference substances. Reported results demonstrate that all compounds displayed antibiofilm activity at the tested concentrations. Remarkable reduction in biofilm formation of P. aeruginosa was found after treatment with KVM-97, KVM-261 and KVM-262 in high concentration (5× MIC), biofilm inhibition activity were 84.3%, 90.5% and 83.3% respectively. After a treatment with KVM-204 at 250 μg/ml (5× MIC) 76.6% of the preformed 24-hr biofilms were destroyed. Furthermore, compounds KVM-97, KVM-194, and KVM-261 in both concentrations showed potent antibiofilm activity against the P. aeruginosa, inhibition activity values being between 56.7 and 65.7%. All tested compounds in dose-dependent manner exhibited pronounced inhibition activity against mature 5-days P. аeruginosa biofilm. It was also observed that tested compounds show high antibiofilm activity in comparison to reference antimicrobials. The aminopropanol derivatives may provide templates for a new group of antimicrobial agents and potential future therapeutics for treating chronic infections.

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Engineering a light-responsive, quorum quenching biofilm to mitigate biofouling on water purification membranes

Science Advances ◽

10.1126/sciadv.aau1459 ◽

2018 ◽

Vol 4 (12) ◽

pp. eaau1459 ◽

Cited By ~ 21

Author(s):

Manisha Mukherjee ◽

Yidan Hu ◽

Chuan Hao Tan ◽

Scott A. Rice ◽

Bin Cao

Keyword(s):

Water Purification ◽

Biofilm Formation ◽

Near Infrared ◽

Forward Osmosis ◽

Quorum Quenching ◽

Bacterial Biofilm ◽

Bacterial Cells ◽

Membrane Biofouling ◽

Purification Membranes ◽

Biofilm Development

Quorum quenching (QQ) has been reported to be a promising approach for membrane biofouling control. Entrapment of QQ bacteria in porous matrices is required to retain them in continuously operated membrane processes and to prevent uncontrollable biofilm formation by the QQ bacteria on membrane surfaces. It would be more desirable if the formation and dispersal of biofilms by QQ bacteria could be controlled so that the QQ bacterial cells are self-immobilized, but the QQ biofilm itself still does not compromise membrane performance. In this study, we engineered a QQ bacterial biofilm whose growth and dispersal can be modulated by light through a dichromatic, optogenetic c-di-GMP gene circuit in which the bacterial cells sense near-infrared (NIR) light and blue light to adjust its biofilm formation by regulating the c-di-GMP level. We also demonstrated the potential application of the engineered light-responsive QQ biofilm in mitigating biofouling of water purification forward osmosis membranes. The c-di-GMP–targeted optogenetic approach for controllable biofilm development we have demonstrated here should prove widely applicable for designing other controllable biofilm-enabled applications such as biofilm-based biocatalysis.

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Influence of Polyphenols on Bacterial Biofilm Formation and Quorum-sensing

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-11-1224 ◽

2003 ◽

Vol 58 (11-12) ◽

pp. 879-884 ◽

Cited By ~ 103

Author(s):

Birgit Huber ◽

Leo Eberl ◽

Walter Feucht ◽

Jürgen Polster

Keyword(s):

Gene Regulation ◽

Quorum Sensing ◽

Biofilm Formation ◽

Pathogenic Bacteria ◽

Bacterial Biofilm ◽

Signal Molecules ◽

Bacterial Cells ◽

Regulatory Systems ◽

Bacterial Quorum Sensing ◽

Bacterial Quorum

Abstract Many bacteria utilize sophisticated regulatory systems to ensure that some functions are only expressed when a particular population density has been reached. The term ‘quorumsensing’ has been coined to describe this form of density-dependent gene regulation which relies on the production and perception of small signal molecules by bacterial cells. As in many pathogenic bacteria the production of virulence factors is quorum-sensing regulated, it has been suggested that this form of gene regulation allows the bacteria to remain invisible to the defence systems of the host until the population is sufficiently large to successfully establish the infection. Here we present first evidence that polyphenolic compounds can interfere with bacterial quorum-sensing. Since polyphenols are widely distributed in the plant kingdom, they may be important for promoting plant fitness.

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Process Description of an Unconventional Biofilm Formation by Bacterial Cells Autoagglutinating Through Sticky, Long, and Peritrichate Nanofibers

10.26434/chemrxiv.10001924.v1 ◽

2019 ◽

Author(s):

Yoshihide Furuichi ◽

Shogo Yoshimoto ◽

Tomohiro Inaba ◽

Nobuhiko Nomura ◽

Katsutoshi Hori

Keyword(s):

Formation Process ◽

Biofilm Formation ◽

Extracellular Polymeric Substances ◽

Waste Treatment ◽

Large Cell ◽

Dlvo Theory ◽

Bacterial Cells ◽

Chemical Production ◽

Discontinuous Surface ◽

Cell Cell

Biofilms are used in environmental biotechnologies including waste treatment and environmentally friendly chemical production. Understanding the mechanisms of biofilm formation is essential to control microbial behavior and improve environmental biotechnologies. Acinetobacter sp. Tol 5 autoagglutinate through the interaction of the long, peritrichate nanofiber protein AtaA, a trimeric autotransporter adhesin. Using AtaA, without cell growth or the production of extracellular polymeric substances, Tol 5 cells quickly form an unconventional biofilm. In this study, we investigated the formation process of this unconventional biofilm, which started with cell–cell interactions, proceeded to cell clumping, and led to the formation of large cell aggregates. The cell–cell interaction was described by DLVO theory based on a new concept, which considers two independent interactions between two cell bodies and between two AtaA fiber tips forming a virtual discontinuous surface. If cell bodies cannot collide owing to an energy barrier at low ionic strengths but approach within the interactive distance of AtaA fibers, cells can agglutinate through their contact. Cell clumping proceeds following the cluster–cluster aggregation model, and an unconventional biofilm containing void spaces and a fractal nature develops. Understanding its formation process would extend the utilization of various types of biofilms, enhancing environmental biotechnologies.

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Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

BMC Research Notes ◽

10.1186/s13104-021-05612-w ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Olivia Nathalia ◽

Diana Elizabeth Waturangi

Keyword(s):

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Biofilm Formation ◽

Pathogenic Bacteria ◽

Vibrio Harveyi ◽

Quorum Quenching ◽

Indicator Bacteria ◽

Antibiofilm Activity ◽

Fish Pathogen ◽

Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

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Bioactive constituents with antibacterial, resistance modulation, anti-biofilm formation and efflux pump inhibition properties from Aidia genipiflora stem bark

Clinical Phytoscience ◽

10.1186/s40816-021-00266-4 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Daniel Anokwah ◽

Evelyn Asante-Kwatia ◽

Abraham Y. Mensah ◽

Cynthia Amaning Danquah ◽

Benjamin K. Harley ◽

...

Keyword(s):

Biofilm Formation ◽

Efflux Pump ◽

Ethyl Acetate Fraction ◽

Stem Bark ◽

Resistance Mechanisms ◽

Bioactive Constituents ◽

Biofilm Inhibition ◽

E Coli ◽

Efflux Pump Inhibition ◽

Oleanonic Acid

Abstract Background Antimicrobial resistance is a global health challenge. The involvement of bacterial biofilms and efflux pumps in the development of multidrug resistance (MDR) is well established. Medicinal plants have been proposed as alternatives for combating MDR focusing on their bioactive constituents with resistance modulatory activities. This study was aimed at investigating the stem bark of Aidia genipiflora for bioactive constituents with anti-biofilm, efflux pump inhibition and resistance modulatory activities. Method The crude methanol extract was purified by column chromatography and isolated compounds characterized by mass and nuclear magnetic resonance spectrometry. Antibacterial activity was determined by the High-throughput spot culture growth inhibition and the broth micro-dilution assay. The ethidium bromide accumulation assay was used to determine efflux pump inhibition property. Biofilm inhibition was determined in a microplate crystal violet retention assay. Results Purification of the ethyl acetate fraction led to the isolation of oleanonic acid (1), 4-hydroxy cinnamic acid docosyl ester (2), β-stigmasterol/β-sitosterol (mixture 3a/b) and D-mannitol (4). The minimum inhibitory concentrations (MICs) ranged from 250 to > 500 μg/mL for extracts and fractions and from 15 to 250 μg/mL for compounds. In the presence of sub-inhibitory concentrations of the compounds, the MIC of amoxicillin against E. coli (20 μg/mL) and P. aeruginosa (320 μg/mL) was reduced by 32 and 10 folds respectively. The whole extract demonstrated anti-biofilm formation and efflux pump inhibition in E. coli, S. aureus and P. aeruginosa. The sterol mixture (3a/b) at concentration of 100 μg/mL caused the highest inhibition (73%) of biofilm formation in S. aureus. Oleanonic acid (1) demonstrated remarkable efflux pump inhibition at MIC of 7.8 μg/mL in E. coli better than the standard drugs verapamil and chlorpromazine. Conclusion This study confirms the prospects of A. genipiflora as a source of new antibacterial agents and adjuvants that could interact with some resistance mechanisms in bacteria to enhance the activity of hitherto ineffective antibiotics. “A small portion of the study has been presented in a conference in the form of poster”.

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