Molecular docking, quorum quenching effect, antibiofilm activity and safety profile of silver-complexed sulfonamide on Pseudomonas aeruginosa

Biofouling ◽  
2021 ◽  
pp. 1-17
Author(s):  
Fallon dos Santos Siqueira ◽  
Camilla Filippi dos Santos Alves ◽  
Alencar Kolinski Machado ◽  
Josiéli Demétrio Siqueira ◽  
Thiago dos Santos ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Docking ◽  
Safety Profile ◽  
Quorum Quenching ◽  
Antibiofilm Activity ◽  
Quenching Effect

scholarly journals Screening and Quantification of Antiquorum-Sensing Activity of Actinobacteria Isolates Against Gram-Positive and Gram-Negative Biofilm Associated Bacteria

2020 ◽  
Author(s):  
Marco Wijaya ◽  
Dea Delicia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Quorum Quenching ◽  
Antibiofilm Activity ◽  
Gram Positive ◽  
Marine Actinobacteria ◽  
Associated Bacteria ◽  
Gram Negative

Abstract Objectives: The purpose of the study is to screen antiquorum sensing activity compound from actinobacteria using Chromobacterium violaceum as indicator and quantify the antibiofilm activity against several biofilm-forming pathogenic bacteria, such as Staphylococcus aureus ATCC 29213, Bacillus cereus ATCC 14579, Enterococcus faecalis ATCC 33186, Vibrio cholerae , Salmonella typhimurium , Pseudomonas aeruginosa ATCC 27853.Results: Crude extracts from marine actinobacteria isolated from Indonesia were promising for treating biofilm-related diseases. Out of 40 isolates 10 were found to have great quorum quenching activity against C. violaceum. Moreover, these isolates were able to inhibit biofim formation by Gram positive and Gram negative pathogenic bacteria. Quorum quenching agents in extracts of 1AC, 14PM, 16PM, 18PM, and CW17 isolates were predicted as proteins or enzymes.

The In Vitro Antibiofilm Activity of Water Leaf Extract of Papaya (Carica papaya L.) against Pseudomonas aeruginosa

2017 ◽  
Vol 2 (3) ◽  
pp. 150-163
Author(s):  
Ekajayanti Kining ◽  
Syamsul Falah ◽  
Novik Nurhidayat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contact Time ◽  
Cell Attachment ◽  
Water Extract ◽  
Opportunistic Pathogen ◽  
Bacterial Biofilm ◽  
Antibiofilm Activity ◽  
Bacterial Survival ◽  
Optimum Conditions

Pseudomonas aeruginosa is one of opportunistic pathogen forming bacterial biofilm. The biofilm sustains the bacterial survival and infections. This study aimed to assess the activity of water extract of papaya leaves on inhibition of cells attachment, growth and degradation of the biofilm using crystal violet (CV) biofilm assay. Research results showed that water extract of papaya leaves contains alkaloids, tanins, flavonoids, and steroids/terpenoids and showed antibacterial activity and antibiofilm against P. aeruginosa. Addition of extract can inhibit the cell attachment and was able to degrade the biofilm of 40.92% and 48.058% respectively at optimum conditions: extract concentration of 25% (v/v), temperature 37.5 °C and contact time 45 minutes. With a concentration of 25% (v/v), temperature of 50 °C and the contact time of 3 days, extract of papaya leaves can inhibit the growth of biofilms of 39.837% v/v.

Confocal and SEM imaging to demonstrate food pathogen- biofilm biocontrol by pyocyanin from Pseudomonas aeruginosa BTRY1

2016 ◽  
Vol 6 (01) ◽  
pp. 5218
Author(s):  
Laxmi Mohandas ◽  
Anju T. R. ◽  
Sarita G. Bhat*
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Antibiofilm Activity ◽  
Opportunistic Pathogens ◽  
Redox Active ◽  
Sem Imaging ◽  
The Impact

An assortment of redox-active phenazine compounds like pyocyanin with their characteristic blue-green colour are synthesized by Pseudomonas aeruginosa, Gram-negative opportunistic pathogens, which are also considered one of the most commercially valuable microorganisms. In this study, pyocyanin from Pseudomonas aeruginosa BTRY1 from food sample was assessed for its antibiofilm activity by micro titer plate assay against strong biofilm producers belonging to the genera Bacillus, Staphylococcus, Brevibacterium and Micrococcus. Pyocyanin inhibited biofilm activity in very minute concentrations. This was also confirmed by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM). Both SEM and CLSM helped to visualize the biocontrol of biofilm formation by eight pathogens. The imaging and quantification by CLSM also established the impact of pyocyanin on biofilm-biocontrol mainly in the food industry.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

scholarly journals Structure–functionality relationship and pharmacological profiles of Pseudomonas aeruginosa alkylquinolone quorum sensing modulators

2017 ◽  
Vol 15 (21) ◽  
pp. 4620-4630 ◽  
Author(s):  
Ahmed A. M. Kamal ◽  
Lucia Petrera ◽  
Jens Eberhard ◽  
Rolf W. Hartmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Docking ◽  
Quorum Sensing ◽  
Structural Requirements ◽  
Inverse Agonists ◽  
Pharmacological Profiles

Alkylquinolone derived compounds revealed four pharmacological profiles for PqsR modulation. Molecular docking illuminated the structural requirements. Only inverse agonists were effective pathoblockers inhibiting pyocyanin.

scholarly journals Phytochemical Profiling of Lavandula coronopifolia Poir. Aerial Parts Extract and Its Larvicidal, Antibacterial, and Antibiofilm Activity Against Pseudomonas aeruginosa

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1710
Author(s):  
Mahmoud Emam ◽  
Doaa R. Abdel-Haleem ◽  
Maha M. Salem ◽  
Lina Jamil M. Abdel-Hafez ◽  
Rasha R. Abdel Latif ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Wild Plant ◽  
Resistant Bacteria ◽  
Control Group ◽  
Antibiofilm Activity ◽  
Left Behind ◽  
Aerial Parts ◽  
Socioeconomic Burden ◽  
Cytochrome P 450

Infections associated with the emergence of multidrug resistance and mosquito-borne diseases have resulted in serious crises associated with high mortality and left behind a huge socioeconomic burden. The chemical investigation of Lavandulacoronopifolia aerial parts extract using HPLC–MS/MS led to the tentative identification of 46 compounds belonging to phenolic acids, flavonoids and their glycosides, and biflavonoids. The extract displayed larvicidal activity against Culex pipiens larvae (LC50 = 29.08 µg/mL at 72 h). It significantly inhibited cytochrome P-450 monooxygenase (CYP450), acetylcholinesterase (AChE), and carboxylesterase (CarE) enzymes with the comparable pattern to the control group, which could explain the mode of larvae toxification. The extract also inhibited the biofilm formation of Pseudomonas aeruginosa by 17–38% at different Minimum Inhibitory Concentrations (MICs) (0.5–0.125 mg/mL) while the activity was doubled when combined with ciprofloxacin (ratio = 1:1 v:v). In conclusion, the wild plant, L.coronopifolia, can be considered a promising natural source against resistant bacteria and infectious carriers.

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