Supercritical CO2 extraction of bioactive compounds from Hibiscus sabdariffa

The potential of supercritical CO2 (SC-CO2) for the extraction of bioactive compounds from mango by-products was assessed. Carotenoid extraction was optimized using a design of experiments based on temperature (35, 55 and 70 °C), pressure (10 and 35 MPa) and co-solvent addition (0%, 10% and 20% of ethanol or acetone). Moreover, the co-extraction of phenolic acids, flavonoids and xanthonoids was evaluated in a subset of parameters. Finally, a comparison was made between SC-CO2 and a two-step organic solvent extraction of the bioactive compounds from the pulp and peel fractions of two Ecuadorian varieties. The optimal extraction temperature was found to be dependent on the bioactive type, with phenolics requiring higher temperature than carotenoids. The optimal overall conditions, focused on maximal carotenoids recovery, were found to be 55 °C, 35 MPa and 20% of ethanol. The main carotenoid was β-carotene, while phenolics differed among the varieties. The bioactive content of the peel was up to 4.1-fold higher than in the pulp fraction. Higher antioxidant activity was found in the extracts obtained with organic solvents. SC-CO2 is a promising technology for the isolation of valuable compounds from mango by-products.

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Characteristics and Empirical Modelling of Extract from Hibiscus sabdariffa Using Supercritical CO2 Extraction with Ethanol-Water as Modifier

Lecture Notes in Mechanical Engineering - Proceedings of the 3rd International Conference on Separation Technology ◽

10.1007/978-981-16-0742-4_26 ◽

2021 ◽

pp. 375-388

Author(s):

Zuhaili Idham ◽

Nicky Rahmana Putra ◽

Nor Faadila Mohd Idrus ◽

Norlisa Mili ◽

Muhammad Syafiq Hazwan Ruslan ◽

...

Keyword(s):

Supercritical Co2 ◽

Hibiscus Sabdariffa ◽

Supercritical Co2 Extraction ◽

Empirical Modelling

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Supercritical CO2 extraction of bioactive compounds from radish leaves: Yield, antioxidant capacity and cytotoxicity

The Journal of Supercritical Fluids ◽

10.1016/j.supflu.2018.01.004 ◽

2018 ◽

Vol 135 ◽

pp. 78-83 ◽

Cited By ~ 20

Author(s):

Rosario Goyeneche ◽

Alejandra Fanovich ◽

Christian Rodriguez Rodrigues ◽

María Celeste Nicolao ◽

Karina Di Scala

Keyword(s):

Antioxidant Capacity ◽

Bioactive Compounds ◽

Supercritical Co2 ◽

Supercritical Co2 Extraction

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Separation of selected bioactive compounds from orange peel using the sequence of supercritical CO2 extraction and ultrasound solvent extraction: optimization of limonene and hesperidin content

Separation Science and Technology ◽

10.1080/01496395.2019.1647245 ◽

2019 ◽

Vol 55 (15) ◽

pp. 2799-2811 ◽

Cited By ~ 2

Author(s):

Stela Jokić ◽

Maja Molnar ◽

Ana-Marija Cikoš ◽

Martina Jakovljević ◽

Silvija Šafranko ◽

...

Keyword(s):

Solvent Extraction ◽

Bioactive Compounds ◽

Supercritical Co2 ◽

Orange Peel ◽

Supercritical Co2 Extraction ◽

Extraction Optimization

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Process optimization of supercritical CO2 extraction of Roselle using response surface methodology

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v16n1.1651 ◽

2020 ◽

Vol 16 (1) ◽

pp. 30-33

Author(s):

Wong Lee Peng ◽

Siti Hamidah Mohd Setapar ◽

Hasmida Mohd Nasir

Keyword(s):

Particle Size ◽

Response Surface Methodology ◽

Response Surface ◽

Supercritical Co2 ◽

Extraction Yield ◽

Hibiscus Sabdariffa ◽

Native Plant ◽

Optimum Conditions ◽

Supercritical Co2 Extraction ◽

Predicted Values

Hibiscus sabdariffa, commonly known as Roselle, is a native plant in Malaysia that is rich with bioactive compounds. In the present study, supercritical carbon dioxide (SC-CO2) extraction of Roselle was investigated. The optimum particle size (212µm, 300µm, 425µm, 600µm, and 710µm) to obtain highest yield was pre-determined. The effects of two operating parameters, pressure (20MPa, 25MPa, and 30MPa) and temperature (40 ºC, 60 ºC, and 80 ºC) on extraction yield were studied using response surface methodology (RSM). From the experimental data, the optimum conditions were achieved using particle size 300µm, pressure 27.5MPa, and temperature 50.8 ºC. Using the optimized parameters, the highest extraction yield was predicted to be 163.26 mg-extract/g-dried sample. The validation experimental results were consistent with the predicted values.

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