Directing a rational design of aptamer-based fluorescence anisotropy assay for sensitive detection of immunoglobulin E by site-specific binding study

Talanta ◽  
2020 ◽  
Vol 217 ◽  
pp. 121018 ◽  
Author(s):  
Qiang Zhao ◽  
Yunlong Bai ◽  
Hailin Wang
Keyword(s):  
Fluorescence Anisotropy ◽  
Rational Design ◽  
Immunoglobulin E ◽  
Specific Binding ◽  
Binding Study ◽  
Sensitive Detection ◽  
Site Specific

scholarly journals Site-Specific Binding of Non-Site-Specific Ions

2019 ◽  
Vol 116 (12) ◽  
pp. 2237-2239 ◽  
Author(s):  
Shi-Jie Chen
Keyword(s):  
Specific Binding ◽  
Site Specific

scholarly journals Site-specific binding of wild-type p53 to cellular DNA is inhibited by SV40 T antigen and mutant p53.

1992 ◽  
Vol 6 (10) ◽  
pp. 1886-1898 ◽  
Author(s):  
J Bargonetti ◽  
I Reynisdottir ◽  
P N Friedman ◽  
C Prives
Keyword(s):  
Specific Binding ◽  
T Antigen ◽  
Mutant P53 ◽  
Wild Type ◽  
Sv40 T Antigen ◽  
Site Specific ◽  
Wild Type P53 ◽  
Cellular Dna

Cds nanocrystal enhanced TiO2photoelectrochemical aptasensor for sensitive detection of cytochrome c

2021 ◽  
Vol 11 (11) ◽  
pp. 1774-1780
Author(s):  
Shanji Fan ◽  
Hong Huang ◽  
Hong Chen ◽  
Jiachi Xu ◽  
Zecheng Hu ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Limit Of Detection ◽  
Specific Binding ◽  
High Sensitivity ◽  
High Specificity ◽  
Linear Range ◽  
Current Intensity ◽  
Sensitive Detection ◽  
Layer Adsorption ◽  
Ionic Layer

A CdS nanocrystal enhanced TiO2 nanotubes (CdS@TiO2 NATs) photoelectrode was prepared via successive ionic layer adsorption and reaction (SILAR) of CdS on the surface of TiO2 NATs. A HS-aptamer owing a specific binding toward cytochrome c was modified onto the CdS@TiO2 NATs, which resulting a decrease in the photoelectrical current intensity. Cytochrome c is therefore quantified based on the decrease in photoelectrical current. High specificity and high sensitivity were obtained with a linear range from 3 pM to 80 nM, and a limit of detection of 2.53 pM.

An electrochemiluminescence aptasensor based on CdSe/ZnS functionalized MoS2 and enzymatic biocatalytic precipitation for sensitive detection of immunoglobulin E

2016 ◽  
Vol 232 ◽  
pp. 538-544 ◽  
Author(s):  
Yan-Ming Liu ◽  
Jiu-Jun Yang ◽  
Jun-Tao Cao ◽  
Jing-Jing Zhang ◽  
Yong-Hong Chen ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Sensitive Detection

Site-Specific N-Glycosylation Analysis of Human Immunoglobulin E

2013 ◽  
Vol 13 (2) ◽  
pp. 536-546 ◽  
Author(s):  
Rosina Plomp ◽  
Paul J. Hensbergen ◽  
Yoann Rombouts ◽  
Gerhild Zauner ◽  
Irina Dragan ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Human Immunoglobulin ◽  
Site Specific

Metal–organic framework MIL-101 enhanced fluorescence anisotropy for sensitive detection of DNA

RSC Advances ◽  
2014 ◽  
Vol 4 (18) ◽  
pp. 9379-9382 ◽  
Author(s):  
Jing Fang Guo ◽  
Chun Mei Li ◽  
Xiao Li Hu ◽  
Cheng Zhi Huang ◽  
Yuan Fang Li
Keyword(s):  
Fluorescence Anisotropy ◽  
Metal Organic Framework ◽  
Sensitive Detection ◽  
Enhanced Fluorescence ◽  
Highly Sensitive ◽  
Metal Organic ◽  
Highly Sensitive Detection ◽  
Organic Framework

MIL-101 was first utilized as a fluorescence anisotropy (FA) amplifier for highly sensitive detection of DNA.

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