Advances in reconstructing intestinal functionalities in vitro: From two/three dimensional-cell culture platforms to human intestine-on-a-chip

Talanta ◽  
2021 ◽  
Vol 226 ◽  
pp. 122097
Author(s):  
Li Wang ◽  
Jian Wu ◽  
Jun Chen ◽  
Wenkun Dou ◽  
Qili Zhao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Human Intestine ◽  
Dimensional Cell

scholarly journals Biomaterials for Three-Dimensional Cell Culture: From Applications in Oncology to Nanotechnology

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 481
Author(s):  
Tarek Saydé ◽  
Omar El Hamoui ◽  
Bruno Alies ◽  
Karen Gaudin ◽  
Gaëtane Lespes ◽  
...  
Keyword(s):  
Cell Culture ◽  
Complex Structure ◽  
Tumor Development ◽  
Three Dimensional ◽  
3D Culture ◽  
Supramolecular Assembly ◽  
Hydrogel Scaffold ◽  
Scale Characterization ◽  
Dimensional Cell

Three-dimensional cell culture has revolutionized cellular biology research and opened the door to novel discoveries in terms of cellular behavior and response to microenvironment stimuli. Different types of 3D culture exist today, including hydrogel scaffold-based models, which possess a complex structure mimicking the extracellular matrix. These hydrogels can be made of polymers (natural or synthetic) or low-molecular weight gelators that, via the supramolecular assembly of molecules, allow the production of a reproducible hydrogel with tunable mechanical properties. When cancer cells are grown in this type of hydrogel, they develop into multicellular tumor spheroids (MCTS). Three-dimensional (3D) cancer culture combined with a complex microenvironment that consists of a platform to study tumor development and also to assess the toxicity of physico-chemical entities such as ions, molecules or particles. With the emergence of nanoparticles of different origins and natures, implementing a reproducible in vitro model that consists of a bio-indicator for nano-toxicity assays is inevitable. However, the maneuver process of such a bio-indicator requires the implementation of a repeatable system that undergoes an exhaustive follow-up. Hence, the biggest challenge in this matter is the reproducibility of the MCTS and the associated full-scale characterization of this system’s components.

A novel in vitro permeability assay using three-dimensional cell culture system

2015 ◽  
Vol 205 ◽  
pp. 93-100 ◽  
Author(s):  
Jong Bong Lee ◽  
Sung Hwa Son ◽  
Min Chul Park ◽  
Tae Hwan Kim ◽  
Min Gi Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Permeability Assay ◽  
Dimensional Cell

scholarly journals Mifepristone, but not levonorgestrel, inhibits human blastocyst attachment to an in vitro endometrial three-dimensional cell culture model

2007 ◽  
Vol 22 (11) ◽  
pp. 3031-3037 ◽  
Author(s):  
P.G.L. Lalitkumar ◽  
S. Lalitkumar ◽  
C.X. Meng ◽  
A. Stavreus-Evers ◽  
F. Hambiliki ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Human Blastocyst ◽  
Culture Model ◽  
Dimensional Cell

scholarly journals Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Xiaowen Wu ◽  
Junxiang Su ◽  
Jizhen Wei ◽  
Nan Jiang ◽  
Xuejun Ge
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Culture Method ◽  
Future Research ◽  
Two Dimensional ◽  
Culture Methods ◽  
Three Dimensional Culture ◽  
Dimensional Cell

Cell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitations of two-dimensional culture and the advantages of three-dimensional culture have increasingly attracted more and more attentions. Compared to two-dimensional culture, three-dimensional culture system is better to realistically simulate the local microenvironment of cells, promote the exchange of information among cells and the extracellular matrix (ECM), and retain the original biological characteristics of stem cells. In this review, we first present three-dimensional cell culture methods from two aspects: a scaffold-free culture system and a scaffold-based culture system. The culture method and cell characterizations will be summarized. Then the application of three-dimensional cell culture system is further explored, such as in the fields of drug screening, organoids and assembloids. Finally, the directions for future research of three-dimensional cell culture are stated briefly.

scholarly journals Two-Dimensional and Three-Dimensional Cell Culture and Their Applications

2021 ◽  
Author(s):  
Sangeeta Ballav ◽  
Ankita Jaywant Deshmukh ◽  
Shafina Siddiqui ◽  
Jyotirmoi Aich ◽  
Soumya Basu
Keyword(s):  
Cell Culture ◽  
Cell Biology ◽  
Low Cost ◽  
Three Dimensional ◽  
3D Culture ◽  
Two Dimensional ◽  
Living Body ◽  
Preclinical Trials ◽  
Dimensional Cell

Cell culture is one of the most important and commonly used in vitro tools to comprehend various aspects of cells or tissues of a living body such as cell biology, tissue morphology, mechanism of diseases, cell signaling, drug action, cancer research and also finds its great importance in preclinical trials of various drugs. There are two major types of cell cultures that are most commonly used- two-dimensional (2D) and three-dimensional culture (3D). The former has been used since the 1900s, owing to its simplicity and low-cost maintenance as it forms a monolayer, while the latter being the advanced version and currently most worked upon. This chapter intends to provide the true meaning and significance to both cultures. It starts by making a clear distinction between the two and proceeds further to discuss their different applications in vitro. The significance of 2D culture is projected through different assays and therapeutic treatment to understand cell motility and treatment of diseases, whereas 3D culture includes different models and spheroid structures consisting of multiple layers of cells, and puts a light on its use in drug discovery and development. The chapter is concluded with a detailed account of the production of therapeutic proteins by the use of cells.

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