Synthesis of aurachin D and isoprenoid analogues from the myxobacterium Stigmatella aurantiaca

2013 ◽  
Vol 54 (49) ◽  
pp. 6700-6702 ◽  
Author(s):  
Lisa Dejon ◽  
Andreas Speicher
2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity

2014 ◽  
Vol 26 (5) ◽  
pp. 491-501 ◽  
Author(s):  
Sandra Pucciarelli ◽  
Federica Chiappori ◽  
Raghul Rajan Devaraj ◽  
Guang Yang ◽  
Ting Yu ◽  
...  

AbstractWe identified two ice-binding protein (IBP) sequences, named EFsymbAFP and EFsymbIBP, from a putative bacterial symbiont of the Antarctic psychrophilic ciliate Euplotes focardii. EFsymbAFP is 57.43% identical to the antifreeze protein (AFP) from the Stigmatella aurantiaca strain DW4/3-1, which was isolated from the Victoria Valley lower glacier. EFsymbIBP is 53.38% identical to the IBP from the Flavobacteriaceae bacterium strain 3519-10, isolated from the glacial ice of Lake Vostok. EFsymbAFP and EFsymbIBP are 31.73% identical at the amino acid level and are organized in tandem on the bacterial chromosome. The relatively low sequence identity and the tandem organization, which appears unique to this symbiont, suggest an occurrence of horizontal gene transfer (HGT). Structurally, EFsymbAFP and EFsymbIBP are similar to the AFPs from the snow mould fungus Typhula ishikariensis and from the Arctic yeast Leucosporidium sp. AY30. A phylogenetic analysis showed that EFsymbAFP and EFsymbIBP cluster principally with the IBP sequences from other Antarctic bacteria, supporting the view that these sequences belong to an Antarctic symbiontic bacterium of E. focardii. These results confirm that IBPs have a complex evolutionary history, which includes HGT events, most probably due to the demands of the environment and the need for rapid adaptation.


1993 ◽  
Vol 10 (5) ◽  
pp. 1087-1099 ◽  
Author(s):  
Björn Neumann ◽  
Andreas Pospiech ◽  
Hans Ulrich Schairer

ChemInform ◽  
2010 ◽  
Vol 32 (36) ◽  
pp. no-no
Author(s):  
Oleg L. Epstein ◽  
Oleg G. Kulinkovich

2013 ◽  
Vol 9 ◽  
pp. 2579-2585 ◽  
Author(s):  
Tobias A M Gulder ◽  
Snežana Neff ◽  
Traugott Schüz ◽  
Tammo Winkler ◽  
René Gees ◽  
...  

The myxobacterial strainStigmatella aurantiacaMYX-030 was selected as promising source for the discovery of new biologically active natural products by our screening methodology. The isolation, structure elucidation and initial biological evaluation of the myxocoumarins derived from this strain are described in this work. These compounds comprise an unusual structural framework and exhibit remarkable antifungal properties.


1988 ◽  
Vol 214 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Ingrid Glomp ◽  
Patrick Saulnier ◽  
Janine Guespin-Michel ◽  
Hans Ulrich Schairer

2006 ◽  
Vol 188 (5) ◽  
pp. 1701-1709 ◽  
Author(s):  
Susanne Müller ◽  
Hui Shen ◽  
Diana Hofmann ◽  
Hans Ulrich Schairer ◽  
John R. Kirby

ABSTRACT Stigmatella aurantiaca displays a complex developmental life cycle in response to starvation conditions that results in the formation of tree-like fruiting bodies capable of producing spores. The phage Mx8, first isolated from the close relative Myxococcus xanthus, is unable to infect S. aurantiaca cells and integrate into the genome. However, plasmids containing Mx8 fragments encoding the integrase and attP are able to integrate at the attB locus in the S. aurantiaca genome by site-specific recombination. After recombination between attP and attB, the S. aurantiaca cells were incapable of building normal fruiting bodies but formed clumps and fungus-like structures characteristic of intermediate stages of development displayed by the wild type. We identified two tRNA genes, trnD and trnV, encoding tRNAAsp and tRNAVal, respectively, composing an operon at the attB locus of S. aurantiaca. Integration of attP-containing plasmids resulted in the incorporation of the tMx8 terminator sequence, in addition to a short sequence of Mx8 DNA downstream of trnD. The integrant was unable to process the trnD transcript at the normal 3′ processing site and displayed a lower level of expression of the trnVD operon. In addition, several developmentally regulated proteins were no longer produced in mutants following insertion at the attB locus. We hypothesize that the integration of the tMx8 terminator sequence results in reduced levels of mature tRNAAsp and tRNAVal and that altered protein production during development is thereby responsible for the observed phenotype. The trnVD locus thus defines a new developmental checkpoint for Stigmatella aurantiaca.


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