Rosmarinic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced apoptosis

The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide ( H 2 O 2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H 2 O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H 2 O 2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H 2 O 2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.

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Rosmarinic acid failed to suppress hydrogen peroxide-mediated apoptosis but induced apoptosis of Jurkat cells which was suppressed by Bcl-2

Molecular and Cellular Biochemistry ◽

10.1007/s11010-005-9064-8 ◽

2006 ◽

Vol 285 (1-2) ◽

pp. 111-120 ◽

Cited By ~ 18

Author(s):

Evangelos Kolettas ◽

Christoforos Thomas ◽

Eleni Leneti ◽

Ioannis Skoufos ◽

Christina Mbatsi ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Rosmarinic Acid ◽

Jurkat Cells ◽

Induced Apoptosis

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Neurotoxicity and Neuroprotective Effects of Polyimides (PI) and Polyphenylenevinylene (PPV) against Hydrogen Peroxide (H2O2)

Scientific Research Journal ◽

10.24191/srj.v8i2.5049 ◽

2011 ◽

Vol 8 (2) ◽

pp. 33

Author(s):

Norfaezah Mazalan ◽

Mazatulikhma Mat Zain ◽

Nor Saliyana Jumali ◽

Norhanim Mohalid ◽

Zurina Shaameri ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Drug Delivery Systems ◽

Neuronal Cells ◽

Water Soluble ◽

Brain Delivery ◽

Specific Therapy ◽

Neuroprotective Effects ◽

Water Soluble Polymer ◽

Site Specific ◽

Novel Drugs

Recently, research and development in the field of drug delivery systems (DDS) facilitating site-specific therapy has reached significant progression. DDS based on polymer micelles, coated micro- and nanoparticles, and various prodrug systems including water-soluble polymer have been prepared and extensively studied as novel drugs designed for cancer chemotherapy and brain delivery. Since polymers are going to be used in human, this study has the interest of testing two types of polymer, polyimides (PI) and polyphenylenevinylene (PPV) on neuronal cells. The objective of this study was to determine the possible neurotoxicity and potential neuroprotective effects of PI and PPV towards SH-SY5Y neuronal cells challenged by hydrogen peroxide (H2O2) as an oxidant. Cells were pretreated with either PI or PPV for 1 hour followed by incubation for 24 hour with 100 µM of H2O2. MTS assay was used to assess cell viability. Results show that PI and PPV are not harmful within the concentration up to 10 µM and 100 µM, respectively. However, PI and PPV do not protect neuronal cells against toxicity induced by H2O2 or further up the cell death.

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