Wood dusts induce the production of reactive oxygen species and caspase-3 activity in human bronchial epithelial cells

Toxicology ◽  
2009 ◽  
Vol 262 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Lea Pylkkänen ◽  
Helene Stockmann-Juvala ◽  
Harri Alenius ◽  
Kirsti Husgafvel-Pursiainen ◽  
Kai Savolainen
Keyword(s):  
Reactive Oxygen Species ◽  
Epithelial Cells ◽  
Caspase 3 ◽  
Reactive Oxygen ◽  
Bronchial Epithelial Cells ◽  
Oxygen Species ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

scholarly journals Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells

Toxics ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 38
Author(s):  
Dong Chang Lee ◽  
Jeong-Min Oh ◽  
Hyunsu Choi ◽  
Sung Won Kim ◽  
Soo Whan Kim ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Particulate Matter ◽  
Epithelial Cells ◽  
Signaling Pathways ◽  
Reactive Oxygen ◽  
Mapk Signaling ◽  
Bronchial Epithelial Cells ◽  
Ros Production ◽  
Oxygen Species ◽  
Bronchial Epithelial

Background: Eupatilin is an active flavon extracted from the Artemisia species and has properties such as antioxidant, anti-inflammatory, and anti-cancer. We examined the effect of eupatilin using fine particulate matter (FPM) and human bronchial epithelial cell line (BEAS-2B) to confirm the potential of eupatilin as a therapeutic agent for respiratory diseases caused by FPM. Methods: Reactive oxygen species (ROS) levels were checked by flow cytometry to identify if FPM and eupatilin affect ROS production. Western blotting was performed to identify the mechanism of action of eupatilin in FPM-exposed BEAS-2B cells. Results: When cells were exposed to FPM above 12.5 μg/mL concentration for 24 h, ROS production increased significantly compared to the control. When eupatilin was added to cells exposed to FPM, the ROS level decreased proportionally with the eupatilin dose. The phosphorylation of Akt, NF-κB p65, and p38 MAPK induced by FPM was significantly reduced by eupatilin, respectively. Conclusion: FPM cause respiratory disease by producing ROS in bronchial epithelial cells. Eupatilin has been shown to inhibit ROS production through altering signaling pathways. The ROS inhibiting property of eupatilin can be exploited in FPM induced respiratory disorders.

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