scholarly journals Trans, Trans-2,4-Decadienal, a Product Found in Cooking Oil Fumes, Induces Cell Proliferation and Cytokine Production Due to Reactive Oxygen Species in Human Bronchial Epithelial Cells

2005 ◽  
Vol 87 (2) ◽  
pp. 337-343 ◽  
Author(s):  
Louis W. Chang ◽  
Wai-Sze Lo ◽  
Pinpin Lin
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Proliferation ◽  
Epithelial Cells ◽  
Cytokine Production ◽  
Bronchial Epithelial Cells ◽  
Oxygen Species ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Cooking Oil ◽  
Cooking Oil Fumes

Wood dusts induce the production of reactive oxygen species and caspase-3 activity in human bronchial epithelial cells

Toxicology ◽  
2009 ◽  
Vol 262 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Lea Pylkkänen ◽  
Helene Stockmann-Juvala ◽  
Harri Alenius ◽  
Kirsti Husgafvel-Pursiainen ◽  
Kai Savolainen
Keyword(s):  
Reactive Oxygen Species ◽  
Epithelial Cells ◽  
Caspase 3 ◽  
Reactive Oxygen ◽  
Bronchial Epithelial Cells ◽  
Oxygen Species ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

scholarly journals The Toxicity of Cooking Oil Fumes on Human Bronchial Epithelial Cells Through ROS-Mediated MAPK, NF-κB Signaling Pathways and NLRP3 Inflammasome

2021 ◽  
Author(s):  
Xiangyu Cao ◽  
Mingyang Fu ◽  
Jingyi Miao ◽  
Yueling Sun ◽  
Rugang Zhu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Western Blot ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Qrt Pcr ◽  
Cooking Oil ◽  
Cooking Oil Fumes

Abstract Cooking oil fumes (COFs) are main pollutants in kitchen and indoor air, which threaten human health. Exposure to COFs can cause respiratory diseases and impair pulmonary function. To investigate the toxicity of COFs on human bronchial epithelial cells (Beas-2B) and explore the underlying mechanisms, MTT assay was conducted to detect the viability of Beas-2B. Intracellular reactive oxygen species (ROS) levels and nitric oxide (NO) levels were determined with DCFH-DA assay and DAF-FM assay. The expression of genes involved in inflammation were measured with quantitative real-time PCR (qRT-PCR). The phosphorylation and the expression of proteins related to Mitogen-activated protein kinase (MAPK), NF-κB signaling pathways were measured with western blot. Our results revealed that COFs decreased Beas-2B cells viability, increased the ROS levels and NO levels in cells and induced apoptosis in Beas-2B cells. The results of qRT-PCR and western blot showed that the expression of NLRP3, p65, iNOS, IL-1β, and the factors related to oxidative stress and inflammation increased, NF-κB signaling pathway and MAPK signaling pathway were activated. This study provided some useful information to alleviate the toxicity of COFs and revealed the possible mechanism for the damage on respiratory system induced by COFs.

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