Oxidative stress, DNA damage and DNA repair capacity in children with Type 1 diabetes mellitus

Background/Objectives. Endothelial dysfunction due to hyperglycemia-induced oxidative damage is an important predictor of future cardiovascular risk in patients with type 1 diabetes mellitus (T1DM) and is present in adolescent T1DM. We hypothesized that combined treatment with the antioxidant vitamins C and E might improve endothelial function (EF) and other biochemical risk factors in adolescents with T1DM.Subjects/Methods. Open-label antioxidant supplementation was given for six weeks with endpoint measurements collected at baseline and study completion. Endpoints measured included EF and plasma measurements of biochemical endothelial risk.Results. Two males and 7 females were studied. Mean age was 12.9 ± 0.9 yrs; mean T1DM duration was 5.5 ± 2.5 yrs; mean BMI was 22.1 ± 3.8 kg/m2; and mean hemoglobin A1c was 9.3 ± 1.1%. No differences were found for EF, high sensitivity CRP, total antioxidant capacity, adiponectin, or endothelial progenitor cells (EPCs) between before and after combined vitamin C and E therapy.Conclusions. Our negative study results do not support previous findings of decreased oxidative damage, improved endothelial function, and increased vascular repair capacity with antioxidant therapy. Longer term studies may be needed to determine the effects, if any, of combined antioxidant therapy on EPCs, EF, and markers of micro- and macrovascular complications in T1DM.

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Parameters of oxidative stress in children with Type 1 diabetes mellitus and their relatives

Journal of Diabetes and its Complications ◽

10.1016/s1056-8727(01)00228-8 ◽

2003 ◽

Vol 17 (1) ◽

pp. 7-10 ◽

Cited By ~ 36

Author(s):

Jana Varvařovská ◽

Jaroslav Racek ◽

František Stožický ◽

Jiřı́ Souček ◽

Ladislav Trefil ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus

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Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage

Nature Communications ◽

10.1038/s41467-019-13167-5 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 8

Author(s):

Emma Bolderson ◽

Joshua T. Burgess ◽

Jun Li ◽

Neha S. Gandhi ◽

Didier Boucher ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Dna Repair ◽

Oxidative Dna Damage ◽

Repair Capacity ◽

Dna Repair Capacity ◽

Dna Repair Protein ◽

Direct Binding ◽

Protein Barrier ◽

Progeria Syndrome

AbstractThe DNA repair capacity of human cells declines with age, in a process that is not clearly understood. Mutation of the nuclear envelope protein barrier-to-autointegration factor 1 (Banf1) has previously been shown to cause a human progeroid disorder, Néstor–Guillermo progeria syndrome (NGPS). The underlying links between Banf1, DNA repair and the ageing process are unknown. Here, we report that Banf1 controls the DNA damage response to oxidative stress via regulation of poly [ADP-ribose] polymerase 1 (PARP1). Specifically, oxidative lesions promote direct binding of Banf1 to PARP1, a critical NAD+-dependent DNA repair protein, leading to inhibition of PARP1 auto-ADP-ribosylation and defective repair of oxidative lesions, in cells with increased Banf1. Consistent with this, cells from patients with NGPS have defective PARP1 activity and impaired repair of oxidative lesions. These data support a model whereby Banf1 is crucial to reset oxidative-stress-induced PARP1 activity. Together, these data offer insight into Banf1-regulated, PARP1-directed repair of oxidative lesions.

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Assessment of biomarkers of inflammation and premature atherosclerosis in adolescents with type-1 diabetes mellitus

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2018-0192 ◽

2019 ◽

Vol 32 (2) ◽

pp. 109-113 ◽

Cited By ~ 6

Author(s):

Ghufran Babar ◽

Mark Clements ◽

Hongying Dai ◽

Geetha Raghuveer

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Glycemic Control ◽

Type 1 Diabetes Mellitus ◽

Pearson Correlation ◽

Premature Coronary Artery Disease ◽

The Impact ◽

Early Atherosclerosis

Abstract Background Type-1 diabetes mellitus (T1DM) causes endothelial dysfunction and early atherosclerosis, which can result in premature coronary artery disease. The aim of this study was to determine the impact of glycemic control, vascular oxidative stress and inflammation on vascular health in adolescents with T1DM. Methods This was a cross-sectional study in adolescents with age- and sex-matched T1DM who were ≥12 years and were at least 2 years post-diagnosis. Recruitment was balanced to include individuals with hemoglobin A1c (HbA1c) ≤8.5% (n=27) or with HbA1c ≥9.5% (n=25). Biomarkers of inflammation were measured in the blood including C-reactive protein (CRP), interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), E-selectin, fibrinogen and tumor necrosis factor-α (TNF-α). Carotid intima media thickness (cIMT) and peripheral arterial tonometry (PAT) were assessed. Results Plasma E-selectin level was significantly different between the two groups with higher levels in the group with HbA1c ≥9.5% (65.0±27.7 ng/mL vs. 48.8±21.5 ng/mL, p=0.02). Though cIMT and PAT were not significantly different between the groups, Pearson correlation showed a significant direct relationship between rising HbA1c and mean right cIMT (p=0.02; r=0.37), PAT (p=0.03, r=0.31) and fibrinogen (p=0.03, r=0.03). Conclusions Elevated E-selectin level is an early marker of oxidative stress in T1DM patients with an elevated HbA1c level. Suboptimal glycemic control as evidenced by a rising HbA1c causes early atherosclerosis.

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Biomarkers of oxidative stress and antioxidant defense in patients with type 1 diabetes mellitus

Ibnosina Journal of Medicine and Biomedical Sciences ◽

10.4103/ijmbs.ijmbs_59_18 ◽

2018 ◽

Vol 10 (6) ◽

pp. 198

Author(s):

Nadia Alghazir ◽

Rabia Alghazeer ◽

Nuri Awayn ◽

Obiyda Ahtiwesh ◽

Sana Elgahmasi

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Type 1 Diabetes Mellitus ◽

Antioxidant Defense ◽

Biomarkers Of Oxidative Stress

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Nanoparticles of beetroot plant extract (Beta vulgaris L.) as an alternative therapy for type 1 diabetes mellitus : A review

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4564 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 2832-2838

Author(s):

Faizal Achmad Hidayat ◽

Herwienda Fatmayoni ◽

Stefani Ayuningtyas ◽

Anggi Muhtar Pratama

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Free Radicals ◽

Type 1 Diabetes Mellitus ◽

Beta Vulgaris ◽

Alternative Therapy ◽

Antidiabetic Activity ◽

The World

Type 1 diabetes mellitus is one of the most common degenerative diseases in the world. Out of 463 million diabetes mellitus patients in the world, 10% of them are type 1 diabetes mellitus patients. One of the complication causes and severity of type 1 diabetes mellitus is oxidative stress. Oxidative stress occurs due to the build-up of excess free radicals obtained from the metabolism of glucose and lipids. Free radicals that cause oxidative stress can be minimized with antioxidants. Beetroot (Beta vulgaris L.) is a plant that is rich in antioxidants and can be used as an alternative therapy for type 1 diabetes mellitus. Various flavonoid contents in beetroot extracts include quercetin, rutin, apigenin, kaempferol, and ferulic acid compounds that have antioxidant and antidiabetic activity. With the evolve of nanoparticle technology, it can be used to increase the solubility of drugs in water, to increase the duration of the drug in the systemic circulation, and to make drug release occurs gradually. The properties of this nanoparticle extract are indispensable for the efficacy and efficiency of treatment. The results of this study provide a lot of information and innovation for the development of the use of beetroot and the treatment of type 1 diabetes mellitus.

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