Sex-dependent gene expression of kidney transporters after Ochratoxin A exposure in F344 rats

The mycotoxin ochratoxin A (OTA) is a potent nephrocarcinogen, mainly in male rats. The aim of this study was to determine the time course of gene expression (GeneChip® Rat Gene 2.0 ST Array, Affymetrix) in kidney samples from male and female F344 rats, treated daily (p.o) with 0.50 mg/kg b.w. (body weight) of OTA for 7 or 21 days, and evaluate if there were differences between both sexes. After OTA treatment, there was an evolution of gene expression in the kidney over time, with more differentially expressed genes (DEG) at 21 days. The gene expression time course was different between sexes with respect to the number of DEG and the direction of expression (up or down): the female response was progressive and consistent over time, whereas males had a different early response with more DEG, most of them up-regulated. The statistically most significant DEG corresponded to metabolism enzymes (Akr1b7, Akr1c2, Adh6 down-regulated in females; Cyp2c11, Dhrs7, Cyp2d1, Cyp2d5 down-regulated in males) or transporters (Slc17a9 down-regulated in females; Slco1a1 (OATP-1) and Slc51b and Slc22a22 (OAT) down-regulated in males). Some of these genes had also a basal sex difference and were over-expressed in males or females with respect to the other sex.

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Gene expression kinetics of renal transporters induced by ochratoxin A in male and female F344 rats

Food and Chemical Toxicology ◽

10.1016/j.fct.2016.10.019 ◽

2016 ◽

Vol 98 ◽

pp. 169-178 ◽

Cited By ~ 6

Author(s):

Laura Pastor ◽

Ariane Vettorazzi ◽

Javier Campión ◽

Paul Cordero ◽

Adela López de Cerain

Keyword(s):

Gene Expression ◽

Ochratoxin A ◽

Male And Female ◽

Renal Transporters ◽

Kinetics Of ◽

F344 Rats ◽

Expression Kinetics

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In vitro gene expression data supporting a DNA non-reactive genotoxic mechanism for ochratoxin A

Toxicology and Applied Pharmacology ◽

10.1016/j.taap.2007.01.008 ◽

2007 ◽

Vol 220 (2) ◽

pp. 216-224 ◽

Cited By ~ 43

Author(s):

Leire Arbillaga ◽

Amaia Azqueta ◽

Joost H.M. van Delft ◽

Adela López de Cerain

Keyword(s):

Gene Expression ◽

Ochratoxin A ◽

Gene Expression Data ◽

Expression Data

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Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix

Fungal Biology ◽

10.1016/j.funbio.2019.11.001 ◽

2019 ◽

Cited By ~ 2

Author(s):

Carla Cervini ◽

Carol Verheecke-Vaessen ◽

Massimo Ferrara ◽

Esther García-Cela ◽

Donato Magistà ◽

...

Keyword(s):

Climate Change ◽

Gene Expression ◽

Ochratoxin A ◽

Secondary Metabolite ◽

Aspergillus Carbonarius ◽

Temperature Cycles ◽

Change Factors

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Ochratoxin A induces craniofacial malformation in mice acting on Dlx5 gene expression

Frontiers in Bioscience ◽

10.2741/e75 ◽

2010 ◽

Vol E2 (1) ◽

pp. 133-142 ◽

Cited By ~ 1

Author(s):

Franco Morelli

Keyword(s):

Gene Expression ◽

Ochratoxin A ◽

Craniofacial Malformation

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In vitro evaluation of essential oils against Aspergillus carbonarius isolates and their effects on Ochratoxin A related gene expression in synthetic grape medium

Food Control ◽

10.1016/j.foodcont.2016.08.016 ◽

2017 ◽

Vol 73 ◽

pp. 71-80 ◽

Cited By ~ 11

Author(s):

Iliada K. Lappa ◽

Eleni Simini ◽

George-John E. Nychas ◽

Efstathios Z. Panagou

Keyword(s):

Gene Expression ◽

Essential Oils ◽

Ochratoxin A ◽

Aspergillus Carbonarius ◽

Related Gene ◽

In Vitro Evaluation

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Microarray analysis of aging-associated immune system alterations in the rostral ventrolateral medulla of F344 rats

Physiological Genomics ◽

10.1152/physiolgenomics.00131.2016 ◽

2017 ◽

Vol 49 (8) ◽

pp. 400-415 ◽

Cited By ~ 5

Author(s):

Sivasai Balivada ◽

Chanran K. Ganta ◽

Yongqing Zhang ◽

Hitesh N. Pawar ◽

Richard J. Ortiz ◽

...

Keyword(s):

Gene Expression ◽

Immune System ◽

Neural Substrates ◽

Rostral Ventrolateral Medulla ◽

Ventrolateral Medulla ◽

Middle Aged ◽

Transcriptomic Profile ◽

Age Related ◽

F344 Rats ◽

Age Related Changes

The rostral ventrolateral medulla (RVLM) is an area of the brain stem that contains diverse neural substrates that are involved in systems critical for physiological function. There is evidence that aging affects some neural substrates within the RVLM, although age-related changes in RVLM molecular mechanisms are not well established. The goal of the present study was to characterize the transcriptomic profile of the aging RVLM and to test the hypothesis that aging is associated with altered gene expression in the RVLM, with an emphasis on immune system associated gene transcripts. RVLM tissue punches from young, middle-aged, and aged F344 rats were analyzed with Agilent’s whole rat genome microarray. The RVLM gene expression profile varied with age, and an association between chronological age and specific RVLM gene expression patterns was observed [ P < 0.05, false discovery rate (FDR) < 0.3]. Functional analysis of RVLM microarray data via gene ontology profiling and pathway analysis identified upregulation of genes associated with immune- and stress-related responses and downregulation of genes associated with lipid biosynthesis and neurotransmission in aged compared with middle-aged and young rats. Differentially expressed genes associated with the complement system and microglial cells were further validated by quantitative PCR with separate RVLM samples ( P < 0.05, FDR < 0.1). The present results have identified age-related changes in the transcriptomic profile of the RVLM, modifications that may provide the molecular backdrop for understanding age-dependent changes in physiological regulation.

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