Assessment of the Heat Input Effect on the Distribution of Temperature Cycles in the HAZ of S460MC Welds in MAG Welding

Temperature cycles generated during welding have a significant effect on the changes in the HAZ of welds, regardless of whether these are changes in structure or mechanical properties; however, it is problematic to obtain temperature cycles with sufficient accuracy across the entire HAZ so that they can be generally taken and used in welding simulations and for real experiments of processes occurring in HAZ. In particular, for a study in a specific location, it is important to know the maximum temperature of the cycle and the cooling rate defined mainly by the parameter t8/5. No studies in which anybody tries to find a mathematical description defining the basic parameters of temperature cycles in the HAZ could be found in the performed research. Therefore, the study presented in this paper results in a mathematical description defining the dependence of achieved maximum temperature on the distance from the fusion line in the HAZ of S460MC welds and with heat input values in the interval from 8 to 14 kJ·cm−1. Moreover, this paper presents the influence of heat input value on the weld pool geometry, including the effect of heat input value on grain coarsening in the highly heated HAZ.

Possibilities to Use Physical Simulations When Studying the Distribution of Residual Stresses in the HAZ of Duplex Steels Welds

Dual phase steels combine very good corrosion resistance with relatively high values of mechanical properties. In addition, they can maintain good plastic properties and toughness at both room temperature and lower temperatures as well. Despite all the advantages mentioned above, their utility properties can be reduced by technological processing, especially by the application of the temperature cycles. As a result, in the material remain residual stresses with local stress peaks, which are quite problematic especially during cyclic loading. Moreover, determining the level and especially the distribution of such residual stresses is very difficult for duplex steels both due to the structure duality and in light of the very small width of the heat-affected zone (HAZ). This is why the paper presents the possibilities of using physical simulations to study the effect of temperature cycles in residual stresses’ magnitude and distribution, where it is possible to study the HAZ in more detail as well as on a much larger sample width due to the utilization of special samples. In the thermal–mechanical simulator Gleeble 3500, temperature-stress cycles were applied to testing samples, generating stress fields with local peaks in the testing samples. In addition, the supplied steel X2CrMnNiN21-5-1 had different phase rations in the individual directions. Therefore, as the residual stresses were measured in several directions and at the same time, it was possible to safely confirm the suitability of the used measurement method. Moreover, the effect of the stress and strain on the change of partial phases’ ratios was observed. It has been experimentally confirmed that annealing temperatures of at least 700 °C are required to eliminate local stress peaks after welding. However, an annealing temperature of 550 °C seems to be optimal to maintain sufficient mechanical properties.

EFFECT OF NATURAL AND ACCELERATED AGING ON THE PROPERTIES OF WOOD-PLASTIC COMPOSITES

The correspondence of natural and laboratory-accelerated aging of WPC has long beenahighly important problem discussed by many scholars. In this work, the changes in moisture content (MC), modulus of rupture (MOR), modulus of elasticity (MOE), screw holding force and creep recovery rates of two groups of wood-plastic composites (WPC) after natural and accelerated aging (high-low temperature cycles and freeze-thaw cycles) were studied to provide guidance for the use of WPC in outdoor applications. The results showed that, after the natural aging and freeze-thaw cycles treatments, MC increased significantly with both 167% of the untreated value of wood-HDPE composites with 30% wood fiber content and a thickness of 25 mm (W25), while 67% and 133% of the wood-HDPE composites with 30% wood fiber content and a thickness of 20 mm (W20), but is almost unchanged after the treatment with high-low temperature cycles. The mechanical strength, including MOR, MOE, screw holding force and creep recovery rate, decreased after natural and accelerated aging. The greatest decreases of MOR, MOE, screw holding force and creep recovery rate were 14%, 13%, 21%, and 7% for W25, while 5%, 8%, 8%, and 14% for W20 respectively. Environmental aging can reduce the strength of WPC, but the bending strength retention rate is more than 85%, showing that performance of WPC is relatively stable compared to wood materials, which is oneof thereasons for the widely use of WPC in outdoor applications.

Production and Purification of Two Bioactive Antimicrobial Peptides Using a Two-Step Approach Involving an Elastin-Like Fusion Tag

Antimicrobial resistance is an increasing global threat, demanding new therapeutic biomolecules against multidrug-resistant bacteria. Antimicrobial peptides (AMPs) are promising candidates for a new generation of antibiotics, but their potential application is still in its infancy, mostly due to limitations associated with large-scale production. The use of recombinant DNA technology for the production of AMPs fused with polymer tags presents the advantage of high-yield production and cost-efficient purification processes at high recovery rates. Owing to their unique properties, we explored the use of an elastin-like recombinamer (ELR) as a fusion partner for the production and isolation of two different AMPs (ABP-CM4 and Synoeca-MP), with an interspacing formic acid cleavage site. Recombinant AMP-ELR proteins were overproduced in Escherichia coli and efficiently purified by temperature cycles. The introduction of a formic acid cleavage site allowed the isolation of AMPs, resorting to a two-step methodology involving temperature cycles and a simple size-exclusion purification step. This simple and easy-to-implement purification method was demonstrated to result in high recovery rates of bioactive AMPs. The minimum inhibitory concentration (MIC) of the free AMPs was determined against seven different bacteria of clinical relevance (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and two Burkholderia cenocepacia strains), in accordance with the EUCAST/CLSI antimicrobial susceptibility testing standards. All the bacterial strains (except for Pseudomonas aeruginosa) were demonstrated to be susceptible to ABP-CM4, including a resistant Burkholderia cenocepacia clinical strain. As for Synoeca-MP, although it did not inhibit the growth of Pseudomonas aeruginosa or Klebsiella pneumoniae, it was demonstrated to be highly active against the remaining bacteria. The present work provides the basis for the development of an efficient and up-scalable biotechnological platform for the production and purification of active AMPs against clinically relevant bacteria.

On The Low-Frequency Elastic Response of Pierre Shale During Temperature Cycles

Summary The impact of temperature on elastic rock properties is less-studied and thus less-understood than that of pressure and stress. Thermal effects on dispersion are experimentally observed herein from seismic to ultrasonic frequencies: Young’s moduli and Poisson’s ratios plus P- and S-wave velocities are determined by forced-oscillation (FO) from 1 to 144 Hz and by pulse-transmission (PT) at 500 kHz. Despite being the dominant sedimentary rock type, shales receive less experimental attention than sandstones and carbonates. To our knowledge, no other FO studies on shale at above ambient temperatures exist. Temperature fluctuations are enforced by two temperature cycles from 20 via 40 to 60○C and vice versa. Measured rock properties are initially irreversible but become reversible with increasing number of heating and cooling segments. Rock property-sensitivity to temperature is likewise reduced. It is revealed that dispersion shifts towards higher frequencies with increasing temperature (reversible if decreased), Young’s moduli and P-wave velocity moduli and P-wave velocity maxima occur at 40○C for frequencies below 56 Hz, and S-wave velocities remain unchanged with temperature (if the first heating segment is neglected) at seismic frequencies. In comparison, ultrasonic P- and S-wave velocities are found to decrease with increasing temperatures. Behavioural differences between seismic and ultrasonic properties are attributed to decreasing fluid viscosity with temperature. We hypothesize that our ultrasonic recordings coincide with the transition-phase separating the low- and high-frequency regimes while our seismic recordings are within the low-frequency regime.

Observation of the rock slope thermal regime, coupled with crackmeter stability monitoring: initial results from three different sites in Czechia (central Europe)

This paper describes a newly designed, experimental, and affordable rock slope monitoring system. This system is being used to monitor three rock slopes in Czechia for a period of up to 2 years. The instrumented rock slopes have different lithology (sandstone, limestone, and granite), aspect, and structural and mechanical properties. Induction crackmeters monitor the dynamic of joints, which separate unstable rock blocks from the rock face. This setup works with a repeatability of measurements of 0.05 mm. External destabilising factors (air temperature, precipitation, incoming and outgoing radiation, etc.) are measured by a weather station placed directly within the rock slope. Thermal behaviour in the rock slope surface zone is monitored using a compound temperature probe, placed inside a 3 m deep subhorizontal borehole, which is insulated from external air temperature. Additionally, one thermocouple is placed directly on the rock slope surface. From the time series measured to date (the longest since autumn 2018), we are able to distinguish differences between the annual and diurnal temperature cycles of the monitored sites. From the first data, a greater annual joint dynamic is measured in the case of larger blocks; however, smaller blocks are more responsive to short-term diurnal temperature cycles. Differences in the thermal regime between the sites are also recognisable and are caused mainly by different slope aspect, rock mass thermal conductivity, and colour. These differences will be explained by the statistical analysis of longer time series in the future.