PCR assay for rapid detection of Pasteurella multocida serogroup A in morbid tissue materials from chickens with fowl cholera

The aim of the present study was to evaluate the prevalence of serogroup A of Pasteurella multocida in Syrian Awassi sheep. Of 1630 samples collected from nasal swabs of healthy and pneumonic sheep (125 herds) and pneumonic sheep lungs, a total of 228 (13.9%) strains were isolated and identified as P. multocida subsp. multocida by phenotypic and biochemical characterisation. However, of them only 117 (51.3%) were identified as serogroup A of P. multocida when PCR assay with specific primers for serogroup A strains was applied. The highest rate of serogroup A isolation was from apparently healthy sheep (49.6%) with consideration that all lung isolates (23 isolates) belonged to serogroup A. Geographical and seasonal distribution showed that about 60% of positively isolated bacteria originated from Syrian desert (29 isolates) and central parts of semi-arid step zone (41 isolates). A significant increase (P≤0.05) in the rate of positive isolates was observed in winter as compared to spring. Pathogenicity tests of 10 isolates with 50 or 10 LD50 values showed that 5 isolates were able to induce symptoms of fowl cholera in challenge-exposed chickens indicating that migratory Awassi sheep might serve as a carrier for serogroup A of P. multocida and that ovine isolates may be virulent for local breed of chickens.

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DEVELOPMENT AND APPLICATION OF Pasteurella multocida SEROTYPE A:1 WHOLE CELL BASED COAGGLUTINATION TEST (CAT) FOR THE RAPID DETECTION OF FOWL CHOLERA CAUSING ORGANISMS

Journal of Experimental Biology and Agricultural Sciences ◽

10.18006/2017.5(6).767.773 ◽

2017 ◽

Vol 5 (6) ◽

pp. 767-773

Author(s):

Stanzin Zadon ◽

◽

Vinod Kumar Chaturvedi ◽

Praveen Kumar Gupta ◽

Varsha Paladan ◽

...

Keyword(s):

Rapid Detection ◽

Pasteurella Multocida ◽

Whole Cell ◽

Serotype A ◽

Fowl Cholera

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Characterisation and comparison of avian Pasteurella multocida strains by conventional and ERIC-PCR assays

Acta Veterinaria Hungarica ◽

10.1556/avet.56.2008.4.1 ◽

2008 ◽

Vol 56 (4) ◽

pp. 429-440 ◽

Cited By ~ 10

Author(s):

Boglárka Sellyei ◽

Zsuzsanna Varga ◽

Éva Ivanics ◽

Tibor Magyar

Keyword(s):

Pasteurella Multocida ◽

Geographical Origin ◽

Host Adaptation ◽

Muscovy Duck ◽

Capsular Type ◽

Pcr Assay ◽

Biochemical Tests ◽

Fowl Cholera ◽

Pcr Assays ◽

Suitable Technique

Sixty-one avian strains ofPasteurella multocidawere characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified asP. multocidasubsp.multocidaand 19 strains (31%) asP. multocidasubsp.septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other capsular types were not identified. From the 61 isolates 24 different fingerprint patterns were generated by ERIC-PCR assay. Based on cluster analysis the strains could be grouped into four larger and four mini-clusters that showed considerable correlation with the geographical origin and the host species. The results indicate that ERIC-PCR may be a suitable technique for studying the host adaptation ofP. multocidaand the epidemiology of fowl cholera.

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Development of a novel real‐time PCR assay targeting p54 gene for rapid detection of African swine fever virus (ASFV) strains circulating in Vietnam

Veterinary Medicine and Science ◽

10.1002/vms3.605 ◽

2021 ◽

Author(s):

Thi Bich Ngoc Trinh ◽

Thang Truong ◽

Van Tam Nguyen ◽

Xuan Dang Vu ◽

Le Anh Dao ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Rapid Detection ◽

African Swine Fever Virus ◽

African Swine Fever ◽

Fever Virus ◽

Pcr Assay

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Novel multi-strain probiotics reduces Pasteurella multocida induced fowl cholera mortality in broilers

Scientific Reports ◽

10.1038/s41598-021-88299-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Rine Christopher Reuben ◽

Shovon Lal Sarkar ◽

Habiba Ibnat ◽

Md. Ali Ahasan Setu ◽

Pravas Chandra Roy ◽

...

Keyword(s):

Growth Performance ◽

Feed Efficiency ◽

Biochemical Parameters ◽

Pasteurella Multocida ◽

White Blood Cells ◽

Clinical Signs ◽

Basal Diet ◽

Prostaglandin E ◽

Fowl Cholera ◽

Anti Inflammatory

AbstractPasteurella multocida causes fowl cholera, a highly contagious poultry disease of global concern, causing significant ecological and economic challenges to the poultry industry each year. This study evaluated the effects of novel multi-strain probiotics consisting of Lactobacillus plantarum, L. fermentum, Pediococcus acidilactici, Enterococcus faecium and Saccharomyces cerevisiae on growth performance, intestinal microbiota, haemato-biochemical parameters and anti-inflammatory properties on broilers experimentally challenged with P. multocida. A total of 120 birds were fed with a basal diet supplemented with probiotics (108 CFU/kg) and then orally challenged with 108 CFU/mL of P. multocida. Probiotics supplementation significantly (P < 0.05) improved growth performance and feed efficiency as well as reducing (P < 0.05) the population of intestinal P. multocida, enterobacteria, and mortality. Haemato-biochemical parameters including total cholesterol, white blood cells (WBC), proteins, glucose, packed cell volume (PCV) and lymphocytes improved (P < 0.05) among probiotic fed birds when compared with the controls. Transcriptional profiles of anti-inflammatory genes including hypoxia inducible factor 1 alpha (HIF1A), tumor necrosis factor- (TNF) stimulated gene-6 (TSG-6) and prostaglandin E receptor 2 (PTGER2) in the intestinal mucosa were upregulated (P < 0.05) in probiotics fed birds. The dietary inclusion of the novel multi-strain probiotics improves growth performance, feed efficiency and intestinal health while attenuating inflammatory reaction, clinical signs and mortality associated with P. multocida infection in broilers.

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