Detection of hepatitis E virus in wild boar (Sus scrofa) livers

2010 ◽  
Vol 186 (2) ◽  
pp. 259-261 ◽  
Author(s):  
Mamadou Kaba ◽  
Bernard Davoust ◽  
Jean-Lou Marié ◽  
Philippe Colson
2019 ◽  
Vol 82 ◽  
pp. 194-201 ◽  
Author(s):  
Simona Di Pasquale ◽  
Paola De Santis ◽  
Giuseppina La Rosa ◽  
Kevin Di Domenico ◽  
Marcello Iaconelli ◽  
...  

2014 ◽  
Vol 50 (2) ◽  
pp. 378-383 ◽  
Author(s):  
Yuka Hara ◽  
Yutaka Terada ◽  
Kenzo Yonemitsu ◽  
Hiroshi Shimoda ◽  
Keita Noguchi ◽  
...  

2008 ◽  
Vol 126 (1-3) ◽  
pp. 74-81 ◽  
Author(s):  
Francesca Martelli ◽  
Andrea Caprioli ◽  
Martina Zengarini ◽  
Andrea Marata ◽  
Caterina Fiegna ◽  
...  

2015 ◽  
Vol 7 ◽  
pp. 41-47 ◽  
Author(s):  
M. Mazzei ◽  
R. Nardini ◽  
R. Verin ◽  
M. Forzan ◽  
A. Poli ◽  
...  

2014 ◽  
Vol 60 (5) ◽  
pp. 835-838 ◽  
Author(s):  
Roberto Nardini ◽  
Ranieri Verin ◽  
Maurizio Mazzei ◽  
Mario Forzan ◽  
Alessandro Poli

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1624
Author(s):  
Mario Forzan ◽  
Maria Irene Pacini ◽  
Marcello Periccioli ◽  
Maurizio Mazzei

Hepatitis E virus (HEV) is a waterborne and foodborne pathogen largely spread around the world. HEV is responsible for acute hepatitis in humans and it is also diffused in domestic and wild animals. In particular, domestic pigs represent the main reservoir of the infection and particular attention should be paid to the consumption of raw and undercooked meat as a possible zoonotic vehicle of the pathogen. Several studies have reported the presence of HEV in wild boar circulating in European countries with similar prevalence rates. In this study, we evaluated the occurrence of HEV in wild boar hunted in specific areas of Tuscany. Sampling was performed by collecting liver samples and also by swabbing the carcasses at the slaughterhouses following hunting activities. Our data indicated that 8/67 (12%) of liver samples and 4/67 (6%) of swabs were positive for HEV RNA. The presence of HEV genome on swabs indicates the possible cross-contamination of carcass surfaces during slaughtering procedures. Altogether, our data indicated that it is essential to promote health education programmes for hunters and consumers to limit the diffusion of the pathogen to humans.


2012 ◽  
Vol 43 (3) ◽  
pp. 640-644 ◽  
Author(s):  
Anuwat Wiratsudakul ◽  
Ladawan Sariya ◽  
Phirom Prompiram ◽  
Siriporn Tantawet ◽  
Duangkhamol Suraruangchai ◽  
...  

2021 ◽  
Vol 9 (11) ◽  
pp. 2302
Author(s):  
Katja Schilling-Loeffler ◽  
Oliver Viera-Segura ◽  
Victor Max Corman ◽  
Julia Schneider ◽  
Ashish K. Gadicherla ◽  
...  

Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described for human isolates so far. Here, five sera and five liver samples from HEV-RNA-positive wild boar samples were inoculated onto PLC/PRF/5 cells, incubated for 3 months and thereafter passaged for additional 6 weeks. As demonstrated by RT-qPCR, immunofluorescence and immune electron microscopy, virus was successfully isolated from two liver samples, which originally contained high HEV genome copy numbers. Both isolates showed slower growth than the culture-adapted HEV strain 47832c. In contrast to this strain, the isolated strains had no insertions in their hypervariable genome region. Next generation sequencing using an HEV sequence-enriched library enabled full genome sequencing. Strain Wb108/17 belongs to subtype 3f and strain Wb257/17 to a tentative novel subtype recently described in Italian wild boars. The results indicate that HEV can be successfully isolated in cell culture from wild boar samples containing high HEV genome copy numbers. The isolates may be used further to study the zoonotic potential of wild boar-derived HEV subtypes.


2018 ◽  
Vol 28 (suppl_4) ◽  
Author(s):  
P De Santis ◽  
S Di Pasquale ◽  
G La Rosa ◽  
D De Medici ◽  
G Micarelli ◽  
...  

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0186858 ◽  
Author(s):  
María A. Risalde ◽  
Antonio Rivero-Juárez ◽  
Fernando Romero-Palomo ◽  
Mario Frías ◽  
Pedro López-López ◽  
...  

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