Pre-existing immunity to pathogenic Listeria monocytogenes does not prevent induction of immune responses to feline immunodeficiency virus by a novel recombinant Listeria monocytogenes vaccine

Vaccine ◽  
2005 ◽  
Vol 23 (12) ◽  
pp. 1479-1490 ◽  
Author(s):  
Rosemary Stevens ◽  
Alora LaVoy ◽  
Shila Nordone ◽  
MaryJo Burkhard ◽  
Gregg A. Dean
Keyword(s):  
Listeria Monocytogenes ◽  
Immune Responses ◽  
Feline Immunodeficiency Virus ◽  
Immunodeficiency Virus

scholarly journals Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected Cats

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 984
Author(s):  
Simões ◽  
LaVoy ◽  
Dean
Keyword(s):  
Immune Response ◽  
Listeria Monocytogenes ◽  
Dendritic Cell ◽  
Innate Immune Response ◽  
Feline Immunodeficiency Virus ◽  
Innate Immune ◽  
Treg Depletion ◽  
Cell Responses ◽  
Immunodeficiency Virus

Regulatory T cells (Treg) are key players in the maintenance of peripheral tolerance, preventing autoimmune diseases and restraining chronic inflammatory diseases. Evidence suggests Treg cells and NK cells have important roles in feline immunodeficiency virus (FIV) pathogenesis; however, in vivo studies investigating the interplay between these two cell populations are lacking. We previously described innate immune defects in FIV-infected cats characterized by cytokine deficits and impaired natural killer cell (NK) and NK T cell (NKT) functions. In this study, we investigated whether in vivo Treg depletion by treatment with an anti-feline CD25 monoclonal antibody would improve the innate immune response against subcutaneous challenge with Listeria monocytogenes (Lm). Treg depletion resulted in an increased overall number of cells in Lm-draining lymph nodes and increased proliferation of NK and NKT cells in FIV-infected cats. Treg depletion did not normalize expression of perforin or granzyme A by NK and NKT cells, nor did Treg depletion result in improved clearance of Lm. Thus, despite the quantitative improvements in the NK and NKT cell responses to Lm, there was no functional improvement in the early control of Lm. CD1a+ dendritic cell percentages in the lymph nodes of FIV-infected cats were lower than in specific-pathogen-free control cats and failed to upregulate CD80 even when Treg were depleted. Taken together, Treg depletion failed to improve the innate immune response of FIV-infected cats against Lm and this may be due to dendritic cell dysfunction.

Infection with feline immunodeficiency virus alters intestinal epithelial transport and mucosal immune responses to probiotics

2013 ◽  
Vol 153 (1-2) ◽  
pp. 146-152 ◽  
Author(s):  
Laura L. Stoeker ◽  
Elizabeth L. Overman ◽  
Shila K. Nordone ◽  
Adam J. Moeser ◽  
Rita D. Simões ◽  
...  
Keyword(s):  
Immune Responses ◽  
Feline Immunodeficiency Virus ◽  
Epithelial Transport ◽  
Intestinal Epithelial ◽  
Immunodeficiency Virus ◽  
Mucosal Immune Responses

Feline Immunodeficiency Virus Gag- and Env-Specific Immune Responses after Vaginal versus Intravenous Infection

2001 ◽  
Vol 17 (18) ◽  
pp. 1767-1778 ◽  
Author(s):  
Mary Jo Burkhard ◽  
Candace K. Mathiason ◽  
Todd Bowdre ◽  
Edward A. Hoover
Keyword(s):  
Immune Responses ◽  
Feline Immunodeficiency Virus ◽  
Immunodeficiency Virus

scholarly journals Oral Immunization with Recombinant Listeria monocytogenes Controls Virus Load after Vaginal Challenge with Feline Immunodeficiency Virus

2004 ◽  
Vol 78 (15) ◽  
pp. 8210-8218 ◽  
Author(s):  
Rosemary Stevens ◽  
Kristina E. Howard ◽  
Sushila Nordone ◽  
MaryJo Burkhard ◽  
Gregg A. Dean
Keyword(s):  
Listeria Monocytogenes ◽  
T Cell ◽  
Feline Immunodeficiency Virus ◽  
High Titer ◽  
Immunoglobulin A ◽  
Oral Immunization ◽  
Intestinal Epithelial ◽  
Wild Type ◽  
Immunodeficiency Virus ◽  
Replication Control

ABSTRACT Recombinant Listeria monocytogenes has many attractive characteristics as a vaccine vector against human immunodeficiency virus (HIV). Wild-type and attenuated Listeria strains expressing HIV Gag have been shown to induce long-lived mucosal and systemic T-cell responses in mice. Using the feline immunodeficiency virus (FIV) model of HIV we evaluated recombinant L. monocytogenes in a challenge system. Five cats were immunized with recombinant L. monocytogenes that expresses the FIV Gag and delivers an FIV Env-expressing DNA vaccine (LMgag/pND14-Lc-env). Control cats were either sham immunized or immunized with wild-type L. monocytogenes (LM-wt). At 1 year after vaginal challenge, provirus could not be detected in any of the nine tissues evaluated from cats immunized with the recombinant bacteria but was detected in at least one tissue in 8 of 10 control animals. Virus was isolated from bone marrow of four of five LMgag/pND14-Lc-env-immunized cats by use of a stringent coculture system but required CD8+ T-cell depletion, indicating CD8+ T-cell suppression of virus replication. Control animals had an inverted CD4:CD8 ratio in mesenteric lymph node and were depleted of both CD4+ and CD8+ intestinal epithelial T cells, while LMgag/pND14-Lc-env-immunized animals showed no such abnormalities. Vaginal FIV-specific immunoglobulin A was present at high titer in three LMgag/pND14-Lc-env-immunized cats before challenge and in all five at 1 year postchallenge. This study demonstrates that recombinant L. monocytogenes conferred some control of viral load after vaginal challenge with FIV.

scholarly journals Prior Puma Lentivirus Infection Modifies Early Immune Responses and Attenuates Feline Immunodeficiency Virus Infection in Cats

Viruses ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 210 ◽  
Author(s):  
Wendy Sprague ◽  
Ryan Troyer ◽  
Xin Zheng ◽  
Britta Wood ◽  
Martha Macmillan ◽  
...  
Keyword(s):  
Virus Infection ◽  
Immune Responses ◽  
Feline Immunodeficiency Virus ◽  
Feline Immunodeficiency Virus Infection ◽  
Immunodeficiency Virus ◽  
Puma Lentivirus
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