High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells

Vaccine ◽  
2014 ◽  
Vol 32 (24) ◽  
pp. 2770-2781 ◽  
Author(s):  
Yvonne Genzel ◽  
Thomas Vogel ◽  
Johannes Buck ◽  
Ilona Behrendt ◽  
Daniel Vazquez Ramirez ◽  
...  
Keyword(s):  
Cell Density ◽  
Influenza A Virus ◽  
Influenza A ◽  
High Cell Density ◽  
Virus Production ◽  
High Cell ◽  
Suspension Cells ◽  
Tangential Flow

scholarly journals Efficient influenza A virus production in high cell density using the novel porcine suspension cell line PBG.PK2.1

Vaccine ◽  
2019 ◽  
Vol 37 (47) ◽  
pp. 7019-7028 ◽  
Author(s):  
Gwendal Gränicher ◽  
Juliana Coronel ◽  
Alexander Pralow ◽  
Pavel Marichal-Gallardo ◽  
Michael Wolff ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Influenza A Virus ◽  
Influenza A ◽  
High Cell Density ◽  
Virus Production ◽  
Suspension Cell ◽  
The Novel ◽  
High Cell ◽  
Suspension Cell Line

scholarly journals High cell density growth of High Five suspension cells in DO-controlled wave-mixed bioreactors

2011 ◽  
Vol 5 (Suppl 8) ◽  
pp. P123 ◽  
Author(s):  
Teddy Beltrametti ◽  
Nicole C Bögli ◽  
Gerhard Greller ◽  
Regine Eibl ◽  
Dieter Eibl
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Suspension Cells ◽  
Density Growth

scholarly journals High-cell-density cultivations to increase MVA virus production

Vaccine ◽  
2018 ◽  
Vol 36 (22) ◽  
pp. 3124-3133 ◽  
Author(s):  
Daniel Vázquez-Ramírez ◽  
Yvonne Genzel ◽  
Ingo Jordan ◽  
Volker Sandig ◽  
Udo Reichl
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Virus Production ◽  
High Cell

scholarly journals Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production

Vaccine ◽  
2019 ◽  
Vol 37 (47) ◽  
pp. 7003-7010 ◽  
Author(s):  
Thomas Bissinger ◽  
Johannes Fritsch ◽  
Adrian Mihut ◽  
Yixiao Wu ◽  
Xuping Liu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Mdck Cells ◽  
Virus Production ◽  
High Cell ◽  
Perfusion Cultures ◽  
Cell Concentrations

scholarly journals Dicer is involved in protection against influenza A virus infection

2007 ◽  
Vol 88 (10) ◽  
pp. 2627-2635 ◽  
Author(s):  
Alexey A. Matskevich ◽  
Karin Moelling
Keyword(s):  
Virus Infection ◽  
Influenza A Virus ◽  
Mammalian Cells ◽  
Influenza A ◽  
Virus Production ◽  
Vero Cells ◽  
Antiviral Response ◽  
Protein Levels ◽  
Infected Cells ◽  
Influenza A Virus Infection

In mammals the interferon (IFN) system is a central innate antiviral defence mechanism, while the involvement of RNA interference (RNAi) in antiviral response against RNA viruses is uncertain. Here, we tested whether RNAi is involved in the antiviral response in mammalian cells. To investigate the role of RNAi in influenza A virus-infected cells in the absence of IFN, we used Vero cells that lack IFN-α and IFN-β genes. Our results demonstrate that knockdown of a key RNAi component, Dicer, led to a modest increase of virus production and accelerated apoptosis of influenza A virus-infected cells. These effects were much weaker in the presence of IFN. The results also show that in both Vero cells and the IFN-producing alveolar epithelial A549 cell line influenza A virus targets Dicer at mRNA and protein levels. Thus, RNAi is involved in antiviral response, and Dicer is important for protection against influenza A virus infection.

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