scholarly journals Correction to: High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells

2021 ◽  
Author(s):  
Xuping Liu
Keyword(s):  
Cell Density ◽  
Influenza A Virus ◽  
Influenza A ◽  
High Cell Density ◽  
Virus Production ◽  
High Yield ◽  
High Cell ◽  
Suspension Cells ◽  
Perfusion Process

High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells

Vaccine ◽  
2014 ◽  
Vol 32 (24) ◽  
pp. 2770-2781 ◽  
Author(s):  
Yvonne Genzel ◽  
Thomas Vogel ◽  
Johannes Buck ◽  
Ilona Behrendt ◽  
Daniel Vazquez Ramirez ◽  
...  
Keyword(s):  
Cell Density ◽  
Influenza A Virus ◽  
Influenza A ◽  
High Cell Density ◽  
Virus Production ◽  
High Cell ◽  
Suspension Cells ◽  
Tangential Flow

scholarly journals Efficient influenza A virus production in high cell density using the novel porcine suspension cell line PBG.PK2.1

Vaccine ◽  
2019 ◽  
Vol 37 (47) ◽  
pp. 7019-7028 ◽  
Author(s):  
Gwendal Gränicher ◽  
Juliana Coronel ◽  
Alexander Pralow ◽  
Pavel Marichal-Gallardo ◽  
Michael Wolff ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Density ◽  
Influenza A Virus ◽  
Influenza A ◽  
High Cell Density ◽  
Virus Production ◽  
Suspension Cell ◽  
The Novel ◽  
High Cell ◽  
Suspension Cell Line

Preparation of Very-High-Yield Recombinant Proteins Using Novel High-Cell-Density Bacterial Expression Methods

2010 ◽  
Vol 2010 (8) ◽  
pp. pdb.prot5475 ◽  
Author(s):  
Victoria Murray ◽  
Jianglei Chen ◽  
Yuefei Huang ◽  
Qianqian Li ◽  
Jianjun Wang
Keyword(s):  
Cell Density ◽  
Recombinant Proteins ◽  
High Cell Density ◽  
Bacterial Expression ◽  
High Yield ◽  
High Cell ◽  
Very High

scholarly journals High cell density growth of High Five suspension cells in DO-controlled wave-mixed bioreactors

2011 ◽  
Vol 5 (Suppl 8) ◽  
pp. P123 ◽  
Author(s):  
Teddy Beltrametti ◽  
Nicole C Bögli ◽  
Gerhard Greller ◽  
Regine Eibl ◽  
Dieter Eibl
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Suspension Cells ◽  
Density Growth

scholarly journals High-cell-density cultivations to increase MVA virus production

Vaccine ◽  
2018 ◽  
Vol 36 (22) ◽  
pp. 3124-3133 ◽  
Author(s):  
Daniel Vázquez-Ramírez ◽  
Yvonne Genzel ◽  
Ingo Jordan ◽  
Volker Sandig ◽  
Udo Reichl
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Virus Production ◽  
High Cell

scholarly journals Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Aida Bakhshi Khalilvand ◽  
Saeed Aminzadeh ◽  
Mohammad Hossein Sanati ◽  
Fereidoun Mahboudi
Keyword(s):  
Escherichia Coli ◽  
Cell Density ◽  
Shake Flask ◽  
High Cell Density ◽  
Fold Increase ◽  
Soluble Expression ◽  
High Yield ◽  
High Cell ◽  
E Coli ◽  
High Cell Density Fermentation

Abstract Background SHuffle is a suitable Escherichia coli (E. coli) strain for high yield cytoplasmic soluble expression of disulfide-bonded proteins such as Insulin due to its oxidative cytoplasmic condition and the ability to correct the arrangement of disulfide bonds. Lispro is an Insulin analog that is conventionally produced in E. coli as inclusion bodies (IBs) with prolonged production time and low recovery. Here in this study, we aimed to optimize cultivation media composition for high cell density fermentation of SHuffle T7 E. coli expressing soluble Lispro proinsulin fused to SUMO tag (SU-INS construct) to obtain high cell density fermentation. Results Factors including carbon and nitrogen sources, salts, metal ions, and pH were screened via Plackett–Burman design for their effectiveness on cell dry weight (CDW) as a measure of cell growth. The most significant variables of the screening experiment were Yeast extract and MgCl2 concentration, as well as pH. Succeedingly, The Central Composite Design was utilized to further evaluate and optimize the level of significant variables. The Optimized media (OM-I) enhanced biomass by 2.3 fold in the shake flask (2.5 g/L CDW) that reached 6.45 g/L (2.6 fold increase) when applied in batch culture fermentation. The efficacy of OM-I media for soluble expression was confirmed in both shake flask and fermentor. Conclusion The proposed media was suitable for high cell density fermentation of E. coli SHuffle T7 and was applicable for high yield soluble expression of Lispro proinsulin.

scholarly journals Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production

Vaccine ◽  
2019 ◽  
Vol 37 (47) ◽  
pp. 7003-7010 ◽  
Author(s):  
Thomas Bissinger ◽  
Johannes Fritsch ◽  
Adrian Mihut ◽  
Yixiao Wu ◽  
Xuping Liu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Mdck Cells ◽  
Virus Production ◽  
High Cell ◽  
Perfusion Cultures ◽  
Cell Concentrations
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