scholarly journals Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS/dsRNA-loaded nanoliposomes

Vaccine ◽  
2014 ◽  
Vol 32 (31) ◽  
pp. 3955-3962 ◽  
Author(s):  
Angels Ruyra ◽  
Mary Cano-Sarabia ◽  
Pablo García-Valtanen ◽  
Daniel Yero ◽  
Isidre Gibert ◽  
...  
Keyword(s):  
Immune System ◽  
Danio Rerio ◽  
Innate Immune System ◽  
Innate Immune ◽  
Stimulation Of

In VivoRadioprotection by 5-Androstenediol: Stimulation of the Innate Immune System

2001 ◽  
Vol 156 (3) ◽  
pp. 283-293 ◽  
Author(s):  
M. H. Whitnall ◽  
C. E. Inal ◽  
W. E. Jackson III ◽  
V. L. Miner ◽  
V. Villa ◽  
...  
Keyword(s):  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Stimulation Of

scholarly journals Neutrophil Migration in the Activation of the Innate Immune Response to DifferentFlavobacterium psychrophilumVaccines in Zebrafish (Danio rerio)

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Camila J. Solís ◽  
Matías Poblete-Morales ◽  
Sergio Cabral ◽  
Juan A. Valdés ◽  
Ariel E. Reyes ◽  
...  
Keyword(s):  
Immune System ◽  
Danio Rerio ◽  
Innate Immune System ◽  
Cold Water ◽  
Neutrophil Migration ◽  
Innate Immune ◽  
Commercial Vaccine ◽  
Bacterial Cold Water Disease ◽  
Effectiveness And Efficiency ◽  
Rainbow Trout Fry Syndrome

Flavobacterium psychrophilumis a Gram-negative bacterium, responsible for the bacterial cold-water disease and the rainbow trout fry syndrome in freshwater salmonid fish. At present, there is only one commercial vaccine in Chile, made with two ChileanF. psychrophilumisolates and another licensed in Europe. The present study analyzed neutrophil migration, as a marker of innate immune activation, in zebrafish (Danio rerio) in response to differentF. psychrophilumbath vaccines, which is the first step in evaluating vaccine effectiveness and efficiency in fish. Results indicated that bacterins of the LM-02-Fp isolate were more immunogenic than those from the LM-13-Fp isolate. However, no differences were observed between the same bacteria inactivated by either formaldehyde or heat. Importantly, the same vaccine formulation without an adjuvant only triggered a mild neutrophil migration compared to the complete vaccine. Observations also found that, after a year of storage at 4°C, the activation of the innate immune system by the different vaccines was considerably decreased. Finally, new vaccine formulations prepared with heat and formaldehyde inactivated LM-02-Fp were significantly more efficient than the available commercial vaccine in regard to stimulating the innate immune system.

scholarly journals Neuroinflammation and disruption in working memory in aged mice after acute stimulation of the peripheral innate immune system

2008 ◽  
Vol 22 (3) ◽  
pp. 301-311 ◽  
Author(s):  
Jing Chen ◽  
Jessica B. Buchanan ◽  
Nathan L. Sparkman ◽  
Jonathan P. Godbout ◽  
Gregory G. Freund ◽  
...  
Keyword(s):  
Working Memory ◽  
Immune System ◽  
Innate Immune System ◽  
Innate Immune ◽  
Aged Mice ◽  
Stimulation Of

scholarly journals The Innate Immune System Is Activated by Stimulation of Vaginal Epithelial Cells with Staphylococcus aureus and Toxic Shock Syndrome Toxin 1

2005 ◽  
Vol 73 (4) ◽  
pp. 2164-2174 ◽  
Author(s):  
Marnie L. Peterson ◽  
Kevin Ault ◽  
Mary J. Kremer ◽  
Aloysius J. Klingelhutz ◽  
Catherine C. Davis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Immune System ◽  
Epithelial Cells ◽  
Innate Immune System ◽  
Toxic Shock Syndrome ◽  
Innate Immune ◽  
Toxic Shock ◽  
Down Regulation ◽  
Vaginal Epithelial Cells ◽  
Stimulation Of

ABSTRACT Despite knowledge of the effects of toxic shock syndrome (TSS) toxin 1 (TSST-1) on the adaptive immune system, little is known about stimulation of the innate immune system, particularly epithelial cells. This study investigated the interactions of TSS Staphylococcus aureus and TSST-1 with human vaginal epithelial cells (HVECs) and porcine mucosal surfaces. When cocultured with HVECs for 6 h, TSS S. aureus MN8 proliferated, formed aggregates on the HVEC surfaces, and produced exotoxins. Receptor binding studies showed that 35S-TSST-1 bound to 5 × 104 receptors per HVEC, with saturation at 15 min. Affymetrix Human GeneChip U133A microarray analysis determined S. aureus MNSM (100 bacteria/HVEC) caused at least twofold up- or down-regulation of 410 HVEC genes by 6 h; these data were also confirmed with S. aureus MN8. TSST-1 (100 μg/ml) caused up- or down-regulation of 2,386 HVEC genes by 6 h. In response to S. aureus, the HVEC genes most up-regulated compared to those in controls were those coding for chemokines or cytokines—MIP-3α, 478-fold; GRO-α, 26-fold; GRO-β, 14-fold; and GRO-γ, 30-fold—suggesting activation of innate immunity. TSST-1 also caused up-regulation of chemokine/cytokine genes. Chemokine/cytokine gene up-regulation was confirmed by enzyme-linked immunosorbent assays measuring the corresponding proteins induced by S. aureus and TSST-1. S. aureus MN8, when incubated with porcine vaginal tissue, increased the flux of 35S-TSST-1 across the mucosal surface. This was accompanied by influx of lymphocytes into the upper layers of the tissue. These data suggest innate immune system activation through epithelial cells, reflected in chemokine/cytokine production and influx of lymphocytes, may cause changes in vaginal mucosa permeability, facilitating TSST-1 penetration.

scholarly journals Kidney Disease and Gout: The Role of the Innate Immune System

2016 ◽  
Vol 9 (1) ◽  
pp. 12-21 ◽  
Author(s):  
William F. Finn
Keyword(s):  
Immune System ◽  
Kidney Disease ◽  
Innate Immune System ◽  
Close Association ◽  
Clinical Manifestations ◽  
Innate Immune ◽  
Tubular Injury ◽  
Renal Tubular ◽  
Stimulation Of

The clinical manifestations and consequence of acute and chronic gout are closely associated with the activation of the innate immune system, stimulation of the NLP3 inflammasome and secretion of interleukin-1β and interleukin-18 via caspace-1 activity. This leads to cytokine release and an inflammatory response. It is now clear that a similar involvement of the innate immune system occurs in many forms of acute and chronic kidney disease with accentuation of renal tubular injury and stimulation of tubulointerstitial fibrosis. The local and systemic activation of the innate immune system may help explain the close association of these conditions and provide a target for therapeutic interdiction.

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