An easy-to-use liquid chromatography method with fluorescence detection for the simultaneous determination of five neuroactive amino acids in different regions of rat brain

2018 ◽  
Vol 91 ◽  
pp. 72-79 ◽  
Author(s):  
Beatriz M. Fonseca ◽  
Ana C. Cristóvão ◽  
Gilberto Alves
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Rat Brain ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Chromatography Method ◽  
Neuroactive Amino Acids ◽  
Liquid Chromatography Method

scholarly journals Simultaneous Determination Of Six Parabens In Cosmetics By A New High Performance Liquid Chromatography Method With Fluorescence Detection

2020 ◽  
Vol 15 (1) ◽  
pp. 27-32
Author(s):  
Pelin Kцseoğlu Yılmaz ◽  
Mehmet Akif Tokat
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
High Performance ◽  
Water Mixture ◽  
Chromatography Method ◽  
Column Temperature ◽  
Liquid Chromatography Method

In this study, a new high performance liquid chromatography method with fluorescence detection was developed and validated for the simultaneous determination of methyl paraben, ethyl paraben, propyl paraben, isopropyl paraben, butyl paraben and benzyl paraben in cosmetics. Separations were achieved using a C18 guard column (2.1 Ч 10 mm, 3 µm) and a C18 analytical column (2.1 Ч 150 mm, 3 µm). Isocratic elution was applied with a mobile phase consisting of 45 % aqueous o-phosphoric acid solution (0.08 %) and 55 % methanol/water mixture (90 : 10 v/v). The excitation and the emission wavelengths were 254 and 310 nm, respectively. Column temperature was fixed at 40 єC. The linear range was 0.50-10.00 μg/mL for all of the parabens. Limits of detection and quantification were in the range of 0.29-0.32 μg/mL and 0.88-0.97 μg/ mL, respectively. Precision and accuracy values were calculated by analysis results of standard solutions at 0.50, 2.50 and 10.00 μg/mL. The developed and validated method was applied for simultaneous quantitative determination of six paraben species in cosmetic tonic and micellar water samples successfully.

scholarly journals A Green HPLC Method for Determination of Nine Sulfonamides in Milk and Beef, and Its Greenness Assessment with Analytical Eco-Scale and Greenness Profile

2020 ◽  
Vol 103 (4) ◽  
pp. 1181-1189
Author(s):  
Xiaoyun Duan ◽  
Xiaofeng Liu ◽  
Yue Dong ◽  
Jing Yang ◽  
Jing Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Animal Origin ◽  
Chromatography Method ◽  
Limit Of Quantitation ◽  
Liquid Chromatography Method ◽  
Animal Origin Foods

Abstract Background Sulfonamides have been widely used in the prevention and clinical treatment of bacterial diseases in livestock and poultry. The use of sulfonamides increases the risk of veterinary drug residues in animal derived foods. The traditional reversed phase liquid chromatography methods for sulfonamides residues detection in animal derived foods have the problem of high consumption of organic solvents. Objective The aim of this study was to establish a green high-performance liquid chromatography method for the detection of sulfonamides residues in different animal-origin foods. Method The sample extraction solutions were purified by the Agela Cleanert PEP-2 cartridge and analyzed by the high-performance liquid chromatography method using ethanol as the green alternative solvent. Results The proposed method was validated in terms of linear range (20–1000 μg/kg), limit of detection (3.0–12.3 μg/kg), limit of quantitation (10–43 μg/kg), accuracy (80.7–101.3%), and repeatability and reproducibility (RSD <5.9% and RSD <8.5% respectively). Conclusions The proposed method is an environmentally friendly, sensitive and reliable high-performance liquid chromatography method for simultaneous determination of sulfonamide residues in animal-origin foods. Highlights In this work, we firstly developed a green high-performance liquid chromatography method for simultaneous determination of the residues of nine sulfonamides in milk and beef with ethanol as the green alternative solvent.

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